Fabrication of heterogeneous biocatalyst tethering artificial prosthetic groups to obtain omega-3-fatty acids by selective hydrolysis of fish oils

RSC Advances ◽  
2016 ◽  
Vol 6 (100) ◽  
pp. 97659-97663 ◽  
Author(s):  
S. Moreno-Pérez ◽  
G. Fernández-Lorente ◽  
O. Romero ◽  
J. M. Guisán ◽  
F. López-Gallego

Immobilized enzymes tethering artificial prosthetic groups improve both the hydrolysis rate and the selectivity towards the eicosapentaenoic acid acyl chains.

2014 ◽  
Vol 5 (9) ◽  
pp. 2004-2019 ◽  
Author(s):  
Trevor A. Mori

Clinical and epidemiological studies provide support that the polyunsaturated omega-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid from fish and fish oils are cardioprotective, particularly in the setting of secondary prevention.


Author(s):  
NNR Beckles-Willson ◽  
T Elliott ◽  
MML Everard

2010 ◽  
Vol 74 (8) ◽  
pp. 1482-1490 ◽  
Author(s):  
C.-A. Castellano ◽  
I. Audet ◽  
J.L. Bailey ◽  
J.-P. Laforest ◽  
J.J. Matte

Nephrology ◽  
2016 ◽  
Vol 21 (3) ◽  
pp. 217-228 ◽  
Author(s):  
Andrea K Viecelli ◽  
Elaine M Pascoe ◽  
Kevan R Polkinghorne ◽  
Carmel M Hawley ◽  
Peta-Anne Paul-Brent ◽  
...  

Catalysts ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1067
Author(s):  
Daniel Andrés-Sanz ◽  
Cristina Fresan ◽  
Gloria Fernández-Lorente ◽  
Javier Rocha-Martín ◽  
Jose M. Guisán

Lecitase Ultra® solutions are mainly composed of bimolecular aggregates of two open structures of the enzyme. The immobilization and fixation of these bimolecular aggregates onto support surfaces is here proposed as a novel protocol for the immobilization and stabilization of Lecitase. The resulting derivatives of Lecitase aggregates were much more stable than the diluted solutions of the enzyme. The most stable of them was obtained by covalent immobilization of the bimolecular aggregate: 300-fold more stable than the diluted enzyme and 75-fold more stable than open Lecitase adsorbed onto hydrophobic supports. The bimolecular aggregate that adsorbed onto polyethyleneimine-agarose exhibited the best combination of activity and stability for the hydrolysis of krill oil. Omega-3 acids are in the sn-2 position of the krill oil, but they are also released by a phospholipase A1 because of migration issues.


Author(s):  
Charlotte Jacobsen ◽  
Anna Frisenfeldt Horn ◽  
Ann-Dorit Moltke Sørensen ◽  
K. H. Sabeena Farvin ◽  
Nina Skall Nielsen

PLoS ONE ◽  
2019 ◽  
Vol 14 (3) ◽  
pp. e0213274
Author(s):  
Andrea K. Viecelli ◽  
Kevan R. Polkinghorne ◽  
Elaine M. Pascoe ◽  
Peta-Anne Paul-Brent ◽  
Carmel M. Hawley ◽  
...  

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