scholarly journals Stabilization of Lecitase Ultra® by Immobilization and Fixation of Bimolecular Aggregates. Release of Omega-3 Fatty Acids by Enzymatic Hydrolysis of Krill Oil

Catalysts ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1067
Author(s):  
Daniel Andrés-Sanz ◽  
Cristina Fresan ◽  
Gloria Fernández-Lorente ◽  
Javier Rocha-Martín ◽  
Jose M. Guisán
Keyword(s):  
Covalent Immobilization ◽  
Omega 3 Fatty Acids ◽  
Krill Oil ◽  
Omega 3 ◽  
Open Structures ◽  
Lecitase Ultra ◽  
Support Surfaces ◽  
Hydrophobic Supports ◽  
Hydrolysis Of ◽  
Derivatives Of

Lecitase Ultra® solutions are mainly composed of bimolecular aggregates of two open structures of the enzyme. The immobilization and fixation of these bimolecular aggregates onto support surfaces is here proposed as a novel protocol for the immobilization and stabilization of Lecitase. The resulting derivatives of Lecitase aggregates were much more stable than the diluted solutions of the enzyme. The most stable of them was obtained by covalent immobilization of the bimolecular aggregate: 300-fold more stable than the diluted enzyme and 75-fold more stable than open Lecitase adsorbed onto hydrophobic supports. The bimolecular aggregate that adsorbed onto polyethyleneimine-agarose exhibited the best combination of activity and stability for the hydrolysis of krill oil. Omega-3 acids are in the sn-2 position of the krill oil, but they are also released by a phospholipase A1 because of migration issues.

Release of Omega-3 Fatty Acids by the Hydrolysis of Fish Oil Catalyzed by Lipases Immobilized on Hydrophobic Supports

2011 ◽  
Vol 88 (8) ◽  
pp. 1173-1178 ◽  
Author(s):  
Gloria Fernández-Lorente ◽  
Lorena Betancor ◽  
Alfonso V. Carrascosa ◽  
Jose M. Guisán
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Hydrophobic Supports ◽  
Hydrolysis Of

Comparative study of tissue deposition of omega-3 fatty acids from polar-lipid rich oil of the microalgae Nannochloropsis oculata with krill oil in rats

2015 ◽  
Vol 6 (1) ◽  
pp. 185-191 ◽  
Author(s):  
Michael L. Kagan ◽  
Aharon Levy ◽  
Alicia Leikin-Frenkel
Keyword(s):  
Fatty Acids ◽  
Comparative Study ◽  
Polar Lipid ◽  
Rat Plasma ◽  
Nannochloropsis Oculata ◽  
Polar Lipids ◽  
Omega 3 Fatty Acids ◽  
Krill Oil ◽  
Omega 3 ◽  
Epa And Dha

An oil from micro-algae rich in EPA with no DHA and consisting of 15% polar lipids (phospholipids and glycolipids) showed equivalent uptake of EPA into rat plasma and organs as omega-3 krill oil consisting of EPA and DHA and 40% phospholipids.

scholarly journals Immobilization of alginate-PAC on Sepabeads EC-HA support

2011 ◽  
Vol 65 (4) ◽  
pp. 431-437 ◽  
Author(s):  
Milena Zuza ◽  
Nenad Milosavic ◽  
Zorica Knezevic-Jugovic
Keyword(s):  
Phenylacetic Acid ◽  
Immobilized Enzyme ◽  
Penicillin Acylase ◽  
Covalent Immobilization ◽  
Enzyme Inactivation ◽  
Penicillin G ◽  
Ph Profile ◽  
Covalently Linked ◽  
Hydrolysis Of ◽  
Derivatives Of

Penicillin acylase (PAC) is an important industrial enzyme for the production of many ?-lactam antibiotics. It is capable of catalyzing the hydrolysis of penicillin G (Pen G) to generate phenylacetic acid (PAA) and 6-aminopenicillanic acid (6-APA). In this paper, in order to prevent enzyme inactivation, an attempt of coupling enzyme modification and immobilization was presented. Chemical modification was promoted to introduce carbohydrate moiety into the PAC molecule, capable of being covalently linked to an amino support. This seems to provide a possibility to couple the enzyme without risking a reaction at the active site which might cause a loss of activity. PAC molecules were modified by cross-linking with polyaldehyde derivatives of alginate in order to add them new and useful functions. Immobilization of alginate-PAC on Sepabeads EC-HA was used as a model system in order to demonstrate the potential of this strategy. Optimal conditions for covalent immobilization of alginate-PAC from Escherichia coli on support Sepabeads EC-HA, were investigated. The immobilized enzyme was then characterized by evaluating the potential effects of immobilization on its thermal stability, temperature and pH profile in comparison with native non-modified PAC and modified non-immobilized PAC. The maximum amount of the alginate-PAC coupled on the dry support of 99 mg/g was satisfactory. Deactivation rate constants at 50 ?C for free PAC, alginate-PAC and alginate-PAC immobilized on Sepabeads EC-HA were 2,32; 50,65 and 1,68 h-1, respectively. Alginate-PAC and alginate-PAC immobilized on Sepabeads EC-HA had the same pH and temperature optimum as the native non-modified PAC.

Fabrication of heterogeneous biocatalyst tethering artificial prosthetic groups to obtain omega-3-fatty acids by selective hydrolysis of fish oils

RSC Advances ◽  
2016 ◽  
Vol 6 (100) ◽  
pp. 97659-97663 ◽  
Author(s):  
S. Moreno-Pérez ◽  
G. Fernández-Lorente ◽  
O. Romero ◽  
J. M. Guisán ◽  
F. López-Gallego
Keyword(s):  
Immobilized Enzymes ◽  
Hydrolysis Rate ◽  
Fish Oils ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Selective Hydrolysis ◽  
Acyl Chains ◽  
Prosthetic Groups ◽  
Hydrolysis Of ◽  
Heterogeneous Biocatalyst

Immobilized enzymes tethering artificial prosthetic groups improve both the hydrolysis rate and the selectivity towards the eicosapentaenoic acid acyl chains.

Physical and oxidation stability of self-emulsifying krill oil-in-water emulsions

2016 ◽  
Vol 7 (8) ◽  
pp. 3590-3598 ◽  
Author(s):  
Qian Wu ◽  
Sibel Uluata ◽  
Leqi Cui ◽  
Chao Wang ◽  
Dongsheng Li ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Oxidation Stability ◽  
Omega 3 Fatty Acids ◽  
Krill Oil ◽  
Omega 3 ◽  
Oil In Water ◽  
Unique Source

Krill oil is a unique source of omega-3 fatty acids since it is a mixture of phospholipids and triacylglycerols.

Electrophilic derivatives of omega-3 fatty acids counteract lung cancer cell growth

2018 ◽  
Vol 81 (4) ◽  
pp. 705-716 ◽  
Author(s):  
Liboria Siena ◽  
Chiara Cipollina ◽  
Serena Di Vincenzo ◽  
Maria Ferraro ◽  
Andreina Bruno ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Fatty Acids ◽  
Cell Growth ◽  
Cancer Cell ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Lung Cancer Cell ◽  
Cancer Cell Growth ◽  
Derivatives Of
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