Hybridization chain reactions on silica coated Qbeads for the colorimetric detection of multiplex microRNAs

2017 ◽  
Vol 53 (36) ◽  
pp. 4954-4957 ◽  
Author(s):  
Qingsheng Guo ◽  
Feika Bian ◽  
Yuqian Liu ◽  
Xiaojun Qu ◽  
Xianyun Hu ◽  
...  
Keyword(s):  
Colorimetric Detection ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Chain Reactions ◽  
Target Binding

An array of Qbead@SiO2 integrated with target binding, hybridization chain reaction and staining achieved colorimetric detection of multiplex miRNAs.

scholarly journals Ultrasensitive colorimetric detection of circulating tumor DNA using hybridization chain reaction and the pivot of triplex DNA

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ruimin Li ◽  
Li Zou ◽  
Yanwei Luo ◽  
Manjun Zhang ◽  
Liansheng Ling
Keyword(s):  
Colorimetric Detection ◽  
Circulating Tumor Dna ◽  
Triplex Dna ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Tumor Dna

scholarly journals Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Congcong Yin ◽  
Qiaoling Zhao ◽  
Aiqin Yue ◽  
Weijun Du ◽  
Dingbin Liu ◽  
...  
Keyword(s):  
Visual Detection ◽  
Colorimetric Detection ◽  
High Specificity ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Class A ◽  
Key Enzyme ◽  
Hairpin Probes ◽  
Type Ab ◽  
Triton X

Soybean saponin is one of the important secondary metabolites in seeds, which has various beneficial physiological functions to human health. GmSg-1 gene is the key enzyme gene for synthesizing class A saponins. It is of great significance to realize the visual and rapid detection of class A saponins at the genetic level. The hybridization chain reaction (HCR) was employed to the visual detection of GmSg-1 gene, which was implemented by changing the length of the target fragment to 92 bp and using the hairpin probes we designed to detect the GmSg-1a and GmSg-1b genes. The best condition of HCR reaction is hemin (1.2 μM), Triton X-100 (0.002%), ABTS (3.8 μM), and H2O2 (1.5 mM). It was found that HCR has high specificity for GmSg-1 gene and could be applied to the visual detection of different soybean cultivars containing Aa type, Ab type, and Aa/Ab type saponins, which could provide technical reference and theoretical basis for molecular breeding of soybean and development of functional soybean products.

Magnetic beads-based DNA hybridization chain reaction amplification and DNAzyme recognition for colorimetric detection of uranyl ion in seafood

2017 ◽  
Vol 956 ◽  
pp. 63-69 ◽  
Author(s):  
Hongyan Zhang ◽  
Xian Cheng ◽  
Lian Chen ◽  
Fan Mo ◽  
LiangJun Xu ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Magnetic Beads ◽  
Colorimetric Detection ◽  
Uranyl Ion ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Reaction Amplification

Colorimetric detection of microRNA based hybridization chain reaction for signal amplification and enzyme for visualization

2017 ◽  
Vol 528 ◽  
pp. 7-12 ◽  
Author(s):  
Na Ying ◽  
Taifan Sun ◽  
Zhibao Chen ◽  
Guangping Song ◽  
Bingyao Qi ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Colorimetric Detection ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

Label-free and enzyme-free colorimetric detection of microRNA by catalyzed hairpin assembly coupled with hybridization chain reaction

2016 ◽  
Vol 81 ◽  
pp. 303-308 ◽  
Author(s):  
Hao Wu ◽  
Yaling Liu ◽  
Hongyong Wang ◽  
Jun Wu ◽  
Feifan Zhu ◽  
...  
Keyword(s):  
Colorimetric Detection ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Catalyzed Hairpin Assembly

Colorimetric Detection of Nucleic Acids through Triplex-Hybridization Chain Reaction and DNA-Controlled Growth of Platinum Nanoparticles on Graphene Oxide

2020 ◽  
Vol 92 (3) ◽  
pp. 2714-2721 ◽  
Author(s):  
Weiwei Chen ◽  
Xiaobo Zhang ◽  
Juanjuan Li ◽  
Lizhen Chen ◽  
Ningning Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nucleic Acids ◽  
Platinum Nanoparticles ◽  
Colorimetric Detection ◽  
Hybridization Chain Reaction ◽  
Controlled Growth ◽  
Chain Reaction

scholarly journals Hybridization Chain Reactions Targeting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

2020 ◽  
Vol 21 (9) ◽  
pp. 3216
Author(s):  
Tzu-Heng Wu ◽  
Chia-Chen Chang ◽  
Ching-Hsu Yang ◽  
Wei-Yin Lin ◽  
Tan Joy Ee ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Gel Electrophoresis ◽  
Rnase P ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Chain Reactions ◽  
Human Rnase ◽  
Local Secondary Structure ◽  
Standard Polymerase Chain Reaction ◽  
Hybridization Efficiency

In this work, hybridization chain reactions (HCRs) toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS–CoV-2) nucleocapsid phosphoproteins gene loci and human RNase P are proposed to provide an isothermal amplification screening tool. The proposed chain reactions target the complementary DNA (cDNA) of SARS–CoV-2, with loci corresponding to gold-standard polymerase chain reaction (PCR) loci. Four hybridization chain reaction reactions are demonstrated herein, targeting N1/N2/N3 loci and human RNase P. The design of the hybridization chain reaction, herein, is assisted with an algorithm. The algorithm helps to search target sequences with low local secondary structure and high hybridization efficiency. The loop domain of the fuel hairpin molecule H1 and H2, which are the tunable segments in such reactions, are used as an optimization parameter to improve the hybridization efficiency of the chain reaction. The algorithm-derived HCR reactions were validated with gel electrophoresis. All proposed reactions exhibit a hybridization complex with a molecular mass >1.5k base pairs, which is clear evidence of chain reaction. The hybridization efficiency trend revealed by gel electrophoresis corresponds nicely to the simulated data from the algorithm. The HCR reactions and the corresponding algorithm serve as a basis to further SARS–CoV-2 sensing applications and facilitate better screening strategies for the prevention of on-going pandemics.

Label-Free and Enzyme-Free Colorimetric Detection of Pb2+ Based on RNA Cleavage and Annealing-Accelerated Hybridization Chain Reaction

2019 ◽  
Vol 91 (7) ◽  
pp. 4806-4813 ◽  
Author(s):  
Zhijun Huang ◽  
Junman Chen ◽  
Zewei Luo ◽  
Xiaqing Wang ◽  
Yixiang Duan
Keyword(s):  
Colorimetric Detection ◽  
Rna Cleavage ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction

scholarly journals Two-color imaging of microRNA with enzyme-free signal amplification via hybridization chain reactions in living cells

2016 ◽  
Vol 7 (3) ◽  
pp. 1940-1945 ◽  
Author(s):  
Lu Li ◽  
Jie Feng ◽  
Haiyun Liu ◽  
Qingling Li ◽  
Lili Tong ◽  
...  
Keyword(s):  
Disease Diagnosis ◽  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Early Disease ◽  
Chain Reaction ◽  
Chain Reactions ◽  
Simultaneous Imaging ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Non Destructive

Here, a non-destructive amplification strategy is developed to image miRNAs in living cells, utilizing the enzyme-free hybridization chain reaction (HCR) with graphene oxide (GO) as a carrier. This provides a new tool for highly sensitive and simultaneous imaging of multiple low-level biomarkers, improving the accuracy of early disease diagnosis.

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