scholarly journals Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Congcong Yin ◽  
Qiaoling Zhao ◽  
Aiqin Yue ◽  
Weijun Du ◽  
Dingbin Liu ◽  
...  
Keyword(s):  
Visual Detection ◽  
Colorimetric Detection ◽  
High Specificity ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Class A ◽  
Key Enzyme ◽  
Hairpin Probes ◽  
Type Ab ◽  
Triton X

Soybean saponin is one of the important secondary metabolites in seeds, which has various beneficial physiological functions to human health. GmSg-1 gene is the key enzyme gene for synthesizing class A saponins. It is of great significance to realize the visual and rapid detection of class A saponins at the genetic level. The hybridization chain reaction (HCR) was employed to the visual detection of GmSg-1 gene, which was implemented by changing the length of the target fragment to 92 bp and using the hairpin probes we designed to detect the GmSg-1a and GmSg-1b genes. The best condition of HCR reaction is hemin (1.2 μM), Triton X-100 (0.002%), ABTS (3.8 μM), and H2O2 (1.5 mM). It was found that HCR has high specificity for GmSg-1 gene and could be applied to the visual detection of different soybean cultivars containing Aa type, Ab type, and Aa/Ab type saponins, which could provide technical reference and theoretical basis for molecular breeding of soybean and development of functional soybean products.

Colorimetric detection of class A soybean saponins by coupling DNAzyme with the gap ligase chain reaction

2020 ◽  
Vol 12 (26) ◽  
pp. 3361-3367
Author(s):  
Wenshuai Li ◽  
Guorui Wu ◽  
Min Wang ◽  
Aiqin Yue ◽  
Weijun Du ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Naked Eye ◽  
Class A

We propose a colorimetric assay based on the coupling of gap ligase chain reaction (Gap-LCR) with DNAzyme to detect the target GmSg-1 genes of class A soybean saponins with the naked eye, without the involvement of expensive instruments.

DNAzyme based visual detection of DNA methylation

2018 ◽  
Vol 54 (14) ◽  
pp. 1710-1713 ◽  
Author(s):  
Mei Li ◽  
Lei Zhang ◽  
Gangyi Chen ◽  
Jing Zhou ◽  
Yi Yuan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Visual Detection ◽  
Colorimetric Detection ◽  
High Specificity ◽  
Specificity And Sensitivity

The colorimetric detection of DNA methylation has been achieved with high specificity and sensitivity by using DNAzyme as a signal reporter.

Hybridization chain reactions on silica coated Qbeads for the colorimetric detection of multiplex microRNAs

2017 ◽  
Vol 53 (36) ◽  
pp. 4954-4957 ◽  
Author(s):  
Qingsheng Guo ◽  
Feika Bian ◽  
Yuqian Liu ◽  
Xiaojun Qu ◽  
Xianyun Hu ◽  
...  
Keyword(s):  
Colorimetric Detection ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Chain Reactions ◽  
Target Binding

An array of Qbead@SiO2 integrated with target binding, hybridization chain reaction and staining achieved colorimetric detection of multiplex miRNAs.

scholarly journals Electrochemical micro-aptasensors for exosome detection based on hybridization chain reaction amplification

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Wenfen Zhang ◽  
Zhenhua Tian ◽  
Shujie Yang ◽  
Joseph Rich ◽  
Shuaiguo Zhao ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Early Stage ◽  
Enzyme Reaction ◽  
High Specificity ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Serum Samples ◽  
Liquid Biopsies ◽  
Early Cancer Diagnosis ◽  
Wide Range

AbstractExosomes are cell-derived nanovesicles that have recently gained popularity as potential biomarkers in liquid biopsies due to the large amounts of molecular cargo they carry, such as nucleic acids and proteins. However, most existing exosome-based analytical sensing methods struggle to achieve high sensitivity and high selectivity simultaneously. In this work, we present an electrochemical micro-aptasensor for the highly sensitive detection of exosomes by integrating a micropatterned electrochemical aptasensor and a hybridization chain reaction (HCR) signal amplification method. Specifically, exosomes are enriched on CD63 aptamer-functionalized electrodes and then recognized by HCR products with avidin-horseradish peroxidase (HRP) attached using EpCAM aptamers as bridges. Subsequently, the current signal that is generated through the enzyme reaction between the HRP enzyme and 3,3’,5,5’-tetramethylbenzidine (TMB)/H2O2 directly correlates to the amount of bound HRP on the HCR products and thus to the number of target exosomes. By introducing anti-EpCAM aptamers, micro-aptasensors can detect cancerous exosomes with high specificity. Due to the micropatterned electrodes and HCR dual-amplification strategy, the micro-aptasensors achieve a linear detection response for a wide range of exosome concentrations from 2.5×103 to 1×107 exosomes/mL, with a detection limit of 5×102 exosomes/mL. Moreover, our method successfully detects lung cancer exosomes in serum samples of early-stage and late-stage lung cancer patients, showcasing the great potential for early cancer diagnosis.

Colorimetric detection of microRNA based hybridization chain reaction for signal amplification and enzyme for visualization

2017 ◽  
Vol 528 ◽  
pp. 7-12 ◽  
Author(s):  
Na Ying ◽  
Taifan Sun ◽  
Zhibao Chen ◽  
Guangping Song ◽  
Bingyao Qi ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Colorimetric Detection ◽  
Hybridization Chain Reaction ◽  
Chain Reaction
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