A novel core–shell hybrid nanostructure was constructed by employing gold nanorod ( AuNR ) combined with rhodamine B (RB) as a core and silica as a shell. The poly(sodium 4-styrenesulfonate) (PSS), a negatively charged polyelectrolyte, played the role of linker to electrostatically trap RB on AuNRs . Due to the fluorescence spectral overlap between RB and AuNRs at 560 nm, the red fluorescence and enhanced green fluorescence of the hybrid nanostructures were observed obviously, which is capable for dual-color labeling. To reduce toxic side effects of AuNRs , silica was coated on AuNRs as a shell to fabricate the novel core–shell hybrid nanostructure function as a dual-color labeling for cancer-cell imaging. The fabricated composite structures were characterized by transmission electron microscopy (TEM), absorption spectrum, fluorescence spectrum, zeta potential measurements and laser scanning confocal microscope (LSCM). The experiment results confirmed that the obtained hybrid nanostructures provided excellent photostability, biocompatibility and active surface for further biological functionalization. The novel composite structures may have great potential application in cell multicolor labeling and imaging instead of traditional fluorescent dyes.
Deterministic assembly of single emitters in sub-5 nanometer optical cavity formed by gold nanorod dimers on three-dimensional DNA origami