A sensitive and regenerative electrochemical immunosensor for quantitative detection of Escherichia coli O157:H7 based on stable polyaniline coated screen-printed carbon electrode and rGO-NR-Au@Pt

2019 ◽  
Vol 11 (11) ◽  
pp. 1475-1482 ◽  
Author(s):  
Xiaoyan Mo ◽  
Zunyi Wu ◽  
Jianfeng Huang ◽  
Guangying Zhao ◽  
Wenchao Dou
Keyword(s):  
Escherichia Coli ◽  
Electrochemical Immunosensor ◽  
Quantitative Detection ◽  
Carbon Electrode ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

An electrochemical immunosensor was constructed for the detection of E. coli O157:H7 using Au@Pt, rGO and regenerative leucoemeraldine PANI/AuNPs.

scholarly journals Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4324 ◽  
Author(s):  
Nurul Talib ◽  
Faridah Salam ◽  
Yusran Sulaiman
Keyword(s):  
Graphene Oxide ◽  
Limit Of Detection ◽  
Food Product ◽  
Electrochemical Immunosensor ◽  
Growth Promoter ◽  
Carbon Electrode ◽  
Electrochemical Assay ◽  
Sensor Platform ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).

Electrochemical Immunosensor Based on Highly Sensitive Amino Functionalized Graphene Nanoplatelets-Modified Screen Printed Carbon Electrode

2020 ◽  
Vol 833 ◽  
pp. 171-175
Author(s):  
Nurul Azurin Badruzaman ◽  
Mohd Azraie Mohd Azmi ◽  
Nur Azura Mohd Said
Keyword(s):  
Incubation Time ◽  
Electrochemical Impedance ◽  
Electrochemical Immunosensor ◽  
Graphene Nanoplatelets ◽  
Carbon Electrode ◽  
Functionalized Graphene ◽  
Working Electrode ◽  
Screen Printed Carbon Electrode ◽  
Rabbit Igg ◽  
Screen Printed

We presented here the development of an immunosensor based on graphene nanoplatelets-modified screen printed carbon electrode (SPCE) with incorporated rabbit IgG on the amino functionalized surface area. In order to improve sensitivity of working electrode, graphene-nanoplatelets solution was fabricated onto surface carbon working electrode. The effect of different (3-aminopropyl) triethoxysilane (APTES) concentrations (0.125, 0.5, 2 and 8% (v/v)) and incubation time of silanization (30, 60 and 90 min) were studied and compared. An electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize our immunosensor based. It is showed that the optimum APTES concentration which provides higher surface coverage and electron transfer rate was 2% concentration (v/v) at 60 min of incubation time. The modified surface was then evaluated by measuring immobilized rabbit IgG via indirect assay using horseradish peroxidase labelled secondary antibody. The optimum detection immobilized IgG was 0.05 mg/mL. These results indicate the potential for amino functionalized graphene nanoplatelets-modified SPCE in detecting protein biomarkers.

Screen-printed carbon electrode-based electrochemical immunosensor for rapid detection of microalbuminuria

2016 ◽  
Vol 77 ◽  
pp. 1175-1182 ◽  
Author(s):  
Jang-Zern Tsai ◽  
Ching-Jung Chen ◽  
Kalpana Settu ◽  
Yu-Feng Lin ◽  
Chien-Lung Chen ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Electrochemical Immunosensor ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

scholarly journals Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode

Sensors ◽  
2013 ◽  
Vol 13 (7) ◽  
pp. 8551-8563 ◽  
Author(s):  
Haiyun Wu ◽  
Yueming Zuo ◽  
Chuanjin Cui ◽  
Wei Yang ◽  
Haili Ma ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Brucella Melitensis ◽  
Quantitative Detection ◽  
Carbon Electrode ◽  
Label Free ◽  
Screen Printed Carbon Electrode ◽  
Impedance Immunosensor ◽  
Screen Printed

A Label-Free Electrochemical Immunosensor Modified with AuNPs for Quantitative Detection of Escherichia coli O157:H7

2019 ◽  
Vol 48 (12) ◽  
pp. 7960-7969 ◽  
Author(s):  
Yanyan Huang ◽  
Zunyi Wu ◽  
Guangying Zhao ◽  
Wenchao Dou
Keyword(s):  
Escherichia Coli ◽  
Electrochemical Immunosensor ◽  
Quantitative Detection ◽  
Escherichia Coli O157 ◽  
Label Free
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