Single-site labeling of histidine in proteins, on-demand reversibility, and traceless metal-free protein purification

2019 ◽  
Vol 55 (8) ◽  
pp. 1100-1103 ◽  
Author(s):  
Pralhad Namdev Joshi ◽  
Vishal Rai
Keyword(s):  
Protein Purification ◽  
Single Site ◽  
Free Protein ◽  
Metal Free ◽  
On Demand ◽  
Multiple Copies

A precision methodology distinguishes one His from all the nucleophilic residues and its multiple copies.

scholarly journals A single amino acid Gly-tag enables metal-free protein purification

2020 ◽  
Vol 11 (48) ◽  
pp. 13137-13142
Author(s):  
Landa Purushottam ◽  
Unnikrishnan V. B. ◽  
Dattatraya Gautam Rawale ◽  
Mansi Gujrati ◽  
Surya Dev Mishra ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Purification ◽  
Single Amino Acid ◽  
Metal Contaminants ◽  
Free Protein ◽  
Metal Free ◽  
N Terminus ◽  
Pure Protein ◽  
User Friendly

Gly-tag resin precisely captures and releases a protein with one glycine at the N-terminus. The user-friendly protocol delivers analytically pure protein free of metal contaminants.

A metal-free yne-addition/1,4-aryl migration/decarboxylation cascade reaction of alkynoates with Csp3–H centers

2016 ◽  
Vol 14 (7) ◽  
pp. 2210-2217 ◽  
Author(s):  
De-Long Kong ◽  
Liang Cheng ◽  
Hong-Ru Wu ◽  
Yang-Xiong Li ◽  
Dong Wang ◽  
...  
Keyword(s):  
Cascade Reaction ◽  
Single Site ◽  
Metal Free ◽  
Different Types

Reactions of alkynoates with different types of radical precursors under metal-free conditions affording single-site-addition poly-substituted alkenes were reported.

Aggregating tags for column-free protein purification

2015 ◽  
Vol 10 (12) ◽  
pp. 1877-1886 ◽  
Author(s):  
Zhanglin Lin ◽  
Qing Zhao ◽  
Lei Xing ◽  
Bihong Zhou ◽  
Xu Wang
Keyword(s):  
Protein Purification ◽  
Free Protein

scholarly journals Expanding One-Pot Cell-Free Protein Synthesis and Immobilization for On-Demand Manufacturing of Biomaterials

2018 ◽  
Vol 7 (3) ◽  
pp. 875-884 ◽  
Author(s):  
Ana I. Benítez-Mateos ◽  
Irantzu Llarena ◽  
Ana Sánchez-Iglesias ◽  
Fernando López-Gallego
Keyword(s):  
Protein Synthesis ◽  
One Pot ◽  
Free Protein ◽  
On Demand ◽  
Cell Free Protein Synthesis

Metal-free catalytic hydrogen production from a polymethylhydrosilane–water mixture

RSC Advances ◽  
2016 ◽  
Vol 6 (7) ◽  
pp. 5903-5906 ◽  
Author(s):  
Chew Pheng Yap ◽  
Hwa Tiong Poh ◽  
Wai Yip Fan
Keyword(s):  
Free Energy ◽  
Hydrogen Production ◽  
Hydrogen Gas ◽  
Energy Carrier ◽  
Water Mixture ◽  
Metal Free ◽  
On Demand ◽  
Formidable Challenge ◽  
Catalytic Hydrogen

Hydrogen gas is the most promising carbon-free energy carrier although its on-demand generation remains a formidable challenge.

Endotoxin-Free E. coli- Based Cell-Free Protein Synthesis: Pre-Expression Endotoxin Removal Approaches for on-Demand Cancer Therapeutic Production

2018 ◽  
Vol 14 (3) ◽  
pp. 1800271 ◽  
Author(s):  
Kristen M. Wilding ◽  
John P. Hunt ◽  
Joshua W. Wilkerson ◽  
Parker J. Funk ◽  
Rebecca L. Swensen ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Free Protein ◽  
E Coli ◽  
On Demand ◽  
Cell Free Protein Synthesis

Single-site labeling of lysine in proteins through a metal-free multicomponent approach

2018 ◽  
Vol 54 (53) ◽  
pp. 7302-7305 ◽  
Author(s):  
Maheshwerreddy Chilamari ◽  
Neetu Kalra ◽  
Sanjeev Shukla ◽  
Vishal Rai
Keyword(s):  
Protein Engineering ◽  
Single Site ◽  
Antibody Drug Conjugates ◽  
Metal Free ◽  
Drug Conjugates ◽  
Antibody Drug

Precise protein engineering is achieved using a metal-free multicomponent approach. It enables the installation of probes and the synthesis of antibody–drug conjugates.

Flexible on-demand cell-free protein synthesis platform based on a tube-in-tube reactor

2020 ◽  
Vol 5 (2) ◽  
pp. 270-277 ◽  
Author(s):  
Caijin Zhou ◽  
Xiaomei Lin ◽  
Yuan Lu ◽  
Jisong Zhang
Keyword(s):  
Protein Synthesis ◽  
Protein Drugs ◽  
Free Protein ◽  
On Demand ◽  
Continuous Synthesis ◽  
Tube Reactor ◽  
Cell Free Protein Synthesis

A flexible on-demand cell-free protein synthesis platform using a tube-in-tube reactor is established for continuous synthesis of different protein drugs.

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