Ultrahigh-throughput droplet microfluidic device for single-cell miRNA detection with isothermal amplification

Lab on a Chip ◽  
2018 ◽  
Vol 18 (13) ◽  
pp. 1914-1920 ◽  
Author(s):  
Song Guo ◽  
Weikang Nicholas Lin ◽  
Yuwei Hu ◽  
Guoyun Sun ◽  
Dinh-Tuan Phan ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Continuous Flow ◽  
Isothermal Amplification ◽  
Mirna Detection

An ultrahigh-throughput single-cell miRNA assay is developed by a continuous-flow microfluidic process employing isothermal amplification to amplify the target miRNA signal.

scholarly journals Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP)

Lab on a Chip ◽  
2015 ◽  
Vol 15 (3) ◽  
pp. 776-782 ◽  
Author(s):  
Tushar D. Rane ◽  
Liben Chen ◽  
Helena C. Zec ◽  
Tza-Huei Wang
Keyword(s):  
Nucleic Acid ◽  
Microfluidic Device ◽  
Continuous Flow ◽  
Isothermal Amplification ◽  
Nucleic Acid Detection ◽  
Loop Mediated Isothermal Amplification

A continuous flow droplet microfluidic device for digital nucleic acid detection using Loop-Mediated Isothermal Amplification (LAMP).

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

High-content screening of drug-induced cardiotoxicity using quantitative single cell imaging cytometry on microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (1) ◽  
pp. 104-114 ◽  
Author(s):  
Min Jung Kim ◽  
Su Chul Lee ◽  
Sukdeb Pal ◽  
Eunyoung Han ◽  
Joon Myong Song
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Imaging ◽  
High Content Screening ◽  
Drug Induced ◽  
Single Cell Imaging ◽  
Imaging Cytometry

scholarly journals Single cell array impedance analysis in a microfluidic device

2016 ◽  
Vol 757 ◽  
pp. 012010
Author(s):  
Emre Altinagac ◽  
Selen Taskin ◽  
Huseyin Kizil
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Impedance Analysis ◽  
Cell Array

scholarly journals Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
B. Deng ◽  
X. F. Li ◽  
D. Y. Chen ◽  
L. D. You ◽  
J. B. Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Single Cell Analysis ◽  
Fine Tuning ◽  
Trapping Efficiency ◽  
Cell Analysis ◽  
Cell Trapping ◽  
Micro Channels ◽  
Single Cell Trapping ◽  
Trapping Sites

Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis. However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization. To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper. By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices. Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency. Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments. Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency. By fine tuning parameters, more than 95% of trapping sites were taken by individual cells. This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.

A valve-based microfluidic device for on-chip single cell treatments

2018 ◽  
Vol 40 (6) ◽  
pp. 961-968 ◽  
Author(s):  
Yue Sun ◽  
Bo Cai ◽  
Xiaoyun Wei ◽  
Zixiang Wang ◽  
Lang Rao ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
On Chip
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