scholarly journals Single-step and ultrasensitive detection of carcinoembryonic antigen based on an aptamer transduction-mediated exonuclease III-assisted dual-amplification strategy

RSC Advances ◽  
2018 ◽  
Vol 8 (26) ◽  
pp. 14663-14668
Author(s):  
Ruojun Lu ◽  
Shengqiang Li ◽  
Meihong Fan ◽  
Jingjing Wei ◽  
Xu Liu
Keyword(s):  
Carcinoembryonic Antigen ◽  
Single Step ◽  
Ultrasensitive Detection ◽  
Exonuclease Iii ◽  
Dual Amplification ◽  
Amplification Strategy

Single-step, rapid and ultrasensitive detection of carcinoembryonic antigen based on an aptamer transduction-mediated exonuclease III-assisted dual-amplification strategy was developed.

A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

2014 ◽  
Vol 50 (13) ◽  
pp. 1576-1578 ◽  
Author(s):  
Liang Cui ◽  
Zhi Zhu ◽  
Ninghang Lin ◽  
Huimin Zhang ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle ◽  
Enzymatic Amplification ◽  
Amplification Method ◽  
Microrna Detection ◽  
Dual Amplification ◽  
Amplification Strategy ◽  
Excellent Selectivity ◽  
T7 Exonuclease

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA–CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

Exonuclease III-assisted substrate fragment recycling amplification strategy for ultrasensitive detection of uranyl by a multipurpose DNAzyme

RSC Advances ◽  
2016 ◽  
Vol 6 (110) ◽  
pp. 108662-108667 ◽  
Author(s):  
Jin-Xiu Cao ◽  
Yong-Sheng Wang ◽  
Jin-Hua Xue ◽  
Yan-Qin Huang ◽  
Ming-Hui Li ◽  
...  
Keyword(s):  
Ultrasensitive Detection ◽  
Exonuclease Iii ◽  
Exo Iii ◽  
Recycling Amplification ◽  
Amplification Strategy

Substrate fragment cleaved by UO22+ hybridizes with SSP6 to form dsDNA, triggering substrate fragment recycling amplification by Exo III.

A dual-amplification fluorescent sensing platform for ultrasensitive assay of nuclease and ATP based on rolling circle replication and exonuclease III-aided recycling

RSC Advances ◽  
2015 ◽  
Vol 5 (92) ◽  
pp. 75055-75061 ◽  
Author(s):  
Chen-Liwei Liu ◽  
Xiang-Juan Kong ◽  
Jing Yuan ◽  
Ru-Qin Yu ◽  
Xia Chu
Keyword(s):  
Rolling Circle Replication ◽  
S1 Nuclease ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Sensing Platform ◽  
Ultrasensitive Assay ◽  
Exo Iii ◽  
Dual Amplification ◽  
Amplification Strategy

A ultrasensitive, easy operated and robust assay of S1 nuclease in real samples and ATP has been successfully achieved with the dual-amplification strategy based on rolling circle replication and Exo III-aided recycling.

Construction of electrochemical immunosensor based on redox hydrogel for ultrasensitive detection of carcinoembryonic antigen

2021 ◽  
Author(s):  
Xiangrong Huang ◽  
Na Wu ◽  
Wenxiu Liu ◽  
Yazhuo Shang ◽  
Honglai Liu ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Carcinoembryonic Antigen ◽  
Electrochemical Detection ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free

In this work, a novel redox hydrogel was proposed for ultrasensitive label-free electrochemical detection of carcinoembryonic antigen (CEA). The redox hydrogel composed by cellulose nanocrystalline (CNC), methylene blue (MB), multi-walled...

A dual amplification strategy for ultrasensitive electrochemiluminescence immunoassay based on a Pt nanoparticles dotted graphene–carbon nanotubes composite and carbon dots functionalized mesoporous Pt/Fe

The Analyst ◽  
2014 ◽  
Vol 139 (7) ◽  
pp. 1713-1720 ◽  
Author(s):  
Wenping Deng ◽  
Fang Liu ◽  
Shenguang Ge ◽  
Jinghua Yu ◽  
Mei Yan ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Carbon Dots ◽  
Pt Nanoparticles ◽  
Electrochemiluminescence Immunoassay ◽  
Dual Amplification ◽  
Amplification Strategy

A facile and sensitive ECL immunosensor has been designed using Pt/Gr–CNTs as a platform and Pt/Fe@CDs as bionanolabels.

A portable signal-on self-powered aptasensor for ultrasensitive detection of sulfadimethoxine based on dual amplification of capacitor and biphotoelectrodes

2021 ◽  
Author(s):  
Yingxu Chen ◽  
Jie Gao ◽  
Xiaoling Yao ◽  
Kai Yan ◽  
Jingdong Zhang
Keyword(s):  
Ultrasensitive Detection ◽  
Self Powered ◽  
Dual Amplification

A one-compartment photofuel cell with two photoelectrodes was combined with a capacitor to develop a portable self-powered sensor for sulfadimethoxine (SDM) detection. The developed sensor was applied to the assay...

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