scholarly journals Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

RSC Advances ◽  
2018 ◽  
Vol 8 (17) ◽  
pp. 9580-9586 ◽  
Author(s):  
Yaning Sun ◽  
Jifei Yang ◽  
Suzhen Yang ◽  
Qingbo Sang ◽  
Man Teng ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Quantitative Detection ◽  
Lateral Flow Strip ◽  
Immunochromatographic Strip ◽  
Gentamicin Sulfate ◽  
Kanamycin Sulfate ◽  
Neomycin Sulfate

A colloidal gold-based immunochromatographic strip has been developed for the rapid, simultaneous, semi-quantitative and quantitative detection of several aminoglycoside residues in milk, including gentamicin sulfate (GM), neomycin sulfate (NEO) and kanamycin sulfate (KN).

scholarly journals A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

2021 ◽  
Vol 18 (9) ◽  
pp. 4574
Author(s):  
Kai Chen ◽  
Biao Ma ◽  
Jiali Li ◽  
Erjing Chen ◽  
Ying Xu ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Quantitative Detection ◽  
Bacterial Strains ◽  
Food Borne Pathogens ◽  
Food Borne

Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Europium nanoparticles (EuNPs) are used as fluorescent probes in lateral flow immunoassays (LFIAs) to improve sensitivity. Here, recombinase polymerase amplification (RPA) combined with fluorescent LFIA was established for the simultaneous and quantitative detection of Listeria monocytogenes, Vibrio parahaemolyticus, and Escherichia coliO157:H7. In this work, the entire experimental process could be completed in 20 min at 37 °C. The limits of detection (LODs) of EuNP-based LFIA–RPA were 9.0 colony-forming units (CFU)/mL for Listeria monocytogenes, 7.0 CFU/mL for Vibrio parahaemolyticus, and 4.0 CFU/mL for Escherichia coliO157:H7. No cross-reaction could be observed in 22 bacterial strains. The fluorescent LFIA–RPA assay exhibits high sensitivity and good specificity. Moreover, the average recovery of the three food-borne pathogens spiked in food samples was 90.9–114.2%. The experiments indicate the accuracy and reliability of the multiple fluorescent test strips. Our developed EuNP-based LFIA–RPA assay is a promising analytical tool for the rapid and simultaneous detection of multiple low concentrations of food-borne pathogens.

scholarly journals Development and Performance verification of colloidal gold labeled SARS-CoV-2 antigen detection method for routine popular screening of COVID-19 with clinical samples in Poland and China

2021 ◽  
Author(s):  
Chuanxiang Guo ◽  
Li Yao ◽  
Fengling Chen ◽  
Chao Zhang ◽  
Wei Chen
Keyword(s):  
Predictive Value ◽  
Colloidal Gold ◽  
Detection Method ◽  
Age Distribution ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Clinical Samples ◽  
Lateral Flow Strip ◽  
And Performance

In this research, we have constructed and optimized the colloidal gold labeled lateral flow strip (LFS) for rapid detection of antigen of SARS-CoV-2 and rapid screening of COVID-19. Based on the constructed and optimized colloidal gold lateral flow strip, the parameters of the LFS have been well evaluated with the clinical samples in the professional labs. The screening performance have also been evaluated from the aspects including the CT values, age distribution and onset of symptoms. Finally, based on the detection results of 420 clinical samples, the LFS can achieve the screening of COVID-19 with the positive percentage agreement (PPA, sensitivity), negative percent agreement (NPA, specificity), the positive predictive value (PPV) and the negative predictive value (NPV) of 96.8%, 100%, 100% and 96.6%, respectively, indicating the powerful potential for practical screening applications in pandemic control. Of great significance, this developed SARS-CoV-2 antigen detection method has also been successfully utilized for screening of delta-variant of SARS-CoV-2.

scholarly journals Development of an antibody-based colloidal gold immunochromatographic lateral flow strip test for natamycin in milk and yoghurt samples

2017 ◽  
Vol 28 (6) ◽  
pp. 1283-1292 ◽  
Author(s):  
Liqiang Liu ◽  
Yanni Chen ◽  
Shanshan Song ◽  
Qiankun Zheng ◽  
Xiaoling Wu ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Strip Test

scholarly journals Simultaneous Detection of Multiple β-Adrenergic Agonists with 2-Directional Lateral Flow Strip Platform

2020 ◽  
Vol 36 (6) ◽  
pp. 653-658
Author(s):  
Qian WU ◽  
Qing SONG ◽  
Xinxin WANG ◽  
Li YAO ◽  
Jianguo XU ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Lateral Flow ◽  
Adrenergic Agonists ◽  
Lateral Flow Strip

scholarly journals Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Klebsiella pneumoniae Serotypes K1 and K2

2016 ◽  
Vol 54 (12) ◽  
pp. 3018-3021 ◽  
Author(s):  
L. Kristopher Siu ◽  
Yu-Kuo Tsai ◽  
Jung-Chung Lin ◽  
Te-Li Chen ◽  
Chang-Phone Fung ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Colloidal Gold ◽  
Capsular Polysaccharide ◽  
Polyclonal Antibodies ◽  
Bacterial Species ◽  
False Negative ◽  
Simultaneous Detection ◽  
Test Methods ◽  
Immunochromatographic Strip ◽  
Content Type

In this study, a novel colloidal gold-based immunochromatographic strip (ICS) containing anti-Klebsiella pneumoniaecapsular polysaccharide polyclonal antibodies was developed to specifically detectK. pneumoniaeserotypes K1 and K2. Capsular polysaccharide K1 and K2 antigens were first used to produce polyclonal anti-K1 and anti-K2 antibodies. Reference strains with different serotypes, nontypeableK. pneumoniaestrains, and other bacterial species were then used to assess the sensitivity and specificity of these test strips. The detection limit was found to be 105CFU, and the ICSs were stable for 6 months when stored at room temperature. No false-positive or false-negative results were observed, and equivalent results were obtained compared to those of more conventional test methods, such as PCR or serum agglutination. In conclusion, the ICS developed here requires no technical expertise and allows for the specific, rapid, and simultaneous detection ofK. pneumoniaeserotypes K1 and K2.

Ultrasensitive detection of seventeen chemicals simultaneously using paper-based sensors

2018 ◽  
Vol 2 (10) ◽  
pp. 1900-1910 ◽  
Author(s):  
Zhongxing Wang ◽  
Li Sun ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Liguang Xu ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Ultrasensitive Detection ◽  
Lateral Flow Strip

We developed an ultrasensitive gold nanoparticle-based multicomponent lateral-flow strip assay for the simultaneous detection of seventeen hormone drugs from three classes: nandrolone (NR) and its analogues, dexamethasone (DEX) and its analogues, and hexestrol (HES) and its analogues.

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