scholarly journals Polymerase-amplified release of ATP (POLARA) for detecting single nucleotide variants in RNA and DNA

2019 ◽  
Vol 10 (11) ◽  
pp. 3264-3270 ◽  
Author(s):  
Michael G. Mohsen ◽  
Debin Ji ◽  
Eric T. Kool
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide Variants ◽  
Single Nucleotide ◽  
Novel Method ◽  
Rna And Dna

ATP-releasing nucleotides are employed to detect single nucleotide polymorphisms in a novel method that is sensitive, rapid, and isothermal.

scholarly journals Differentiating Genomic SNPs Using Allele Depth and Predicted Genotype

2018 ◽  
Author(s):  
Saam Hasan
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Rna Editing ◽  
Editing Site ◽  
Biological Processes ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide Variants ◽  
Accuracy Rate ◽  
Single Nucleotide

AbstractDifferentiating between genomic SNPs and other types of single nucleotide variants becomes a key issue in research aimed at studying the importance of these variants of a particular type in biological processes. Here we present an R based method for differentiating between genomic single nucleotide polymorphisms (SNPs) and RNA editing sites. We use data from an earlier study of ours and target only the known dbsnp SNPs that we found in our study. Our method involves calculating the ratio of allele depth for ref and alt alleles and comparing that to the predicted genotype. We use the concept that editing levels should be different for each allele and thus should not reflect the ratio predicted by the genotype. The study yielded an accuracy rate ranging from 86 to over 90 percent at successfully predicted dbsnp entries as SNPs. Albeit this is in the absence of known RNA editing site vcf data to compare as a reference.

Development of a simple method for the determination of single nucleotide polymorphisms

2010 ◽  
Vol 34 (8) ◽  
pp. S75-S75
Author(s):  
Weifeng Zhu ◽  
Zhuoqi Liu ◽  
Daya Luo ◽  
Xinyao Wu ◽  
Fusheng Wan
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Simple Method ◽  
Single Nucleotide
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