Efficient capture and photothermal ablation of planktonic bacteria and biofilms using reduced graphene oxide–polyethyleneimine flexible nanoheaters

2019 ◽  
Vol 7 (17) ◽  
pp. 2771-2781 ◽  
Author(s):  
Milica Budimir ◽  
Roxana Jijie ◽  
Ran Ye ◽  
Alexandre Barras ◽  
Sorin Melinte ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Gram Positive ◽  
Nanohole Array ◽  
Gram Negative ◽  
Planktonic Bacteria ◽  
Photothermal Ablation ◽  
Reduced Graphene

A flexible nanoheater device, consisting of a Au nanohole array coated with reduced graphene oxide–polyethyleneimine, was applied to capture and eradicate both Gram-positive and Gram negative planktonic bacteria and their biofilms.

scholarly journals Reduced graphene oxide-chitosan flexible nanocomposites for efficient bacteria capture and photothermal ablation

2020 ◽  
Vol 5 (1) ◽  
pp. 5-12
Author(s):  
Milica Budimir ◽  
Duška Kleut ◽  
Biljana Todorovic Markovic ◽  
Rabah Boukherroub
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Electrostatic Interactions ◽  
Near Infrared ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Antibiotic Resistant ◽  
Gram Negative ◽  
Photothermal Ablation ◽  
Reduced Graphene

AbstractOne of the major public health concerns today is bacterial infection-associated diseases. Traditional antibacterial therapies are becoming less efficient because frequent and inadequate use of antibiotics has caused mutations in bacteria that led to many antibiotic-resistant bacterial strains. It is, therefore, crucial to develop novel antibacterial materials and strategies that will successfully combat both gram-positive and gram-negative bacteria. In the present study, we will demonstrate a simple and efficient method for bacteria capture and elimination through photothermal ablation. The developed material consists of a flexible Kapton substrate, coated with reduced graphene oxide-chitosan (rGO-CS) thin films. Reduced graphene oxide has strong absorption in the near-infrared (NIR) region, while chitosan has the ability to bind bacteria through electrostatic interactions. The K/rGO-CS device proved to capture and efficiently eradicate both planktonic Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria after 10 min of NIR (980 nm) irradiation.

scholarly journals Synthesis and Characterization of Sulfur and Sulfur-Selenium Nanoparticles Loaded on Reduced Graphene Oxide and Their Antibacterial Activity against Gram-Positive Pathogens

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 191
Author(s):  
Rashmi Niranjan ◽  
Saad Zafar ◽  
Bimlesh Lochab ◽  
Richa Priyadarshini
Keyword(s):  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Spherical Shape ◽  
Dependent Manner ◽  
Gram Positive ◽  
Concentration Dependent Manner ◽  
Reduced Graphene

Resistance to antimicrobial agents in Gram-positive bacteria has become a major concern in the last decade. Recently, nanoparticles (NP) have emerged as a potential solution to antibiotic resistance. We synthesized three reduced graphene oxide (rGO) nanoparticles, namely rGO, rGO-S, and rGO-S/Se, and characterized them using X-ray diffraction (PXRD), Raman analysis, and thermogravimetric analysis. Transmission electron microscopy confirmed spherical shape nanometer size S and S/Se NPs on the rGO surface. Antibacterial properties of all three nanomaterials were probed against Gram-positive pathogens Staphylococcus aureus and Enterococcus faecalis, using turbidometeric and CFU assays. Among the synthesized nanomaterials, rGO-S/Se exhibited relatively strong antibacterial activity against both Gram-positive microorganism tested in a concentration dependent manner (growth inhibition >90% at 200 μg/mL). Atomic force microscopy of rGO-S/Se treated cells displayed morphological aberrations. Our studies also revealed that rGO composite NPs are able to deposit on the bacterial cell surface, resulting in membrane perturbation and oxidative stress. Taken together, our results suggest a possible three-pronged approach of bacterial cytotoxicity by these graphene-based materials.

scholarly journals Graphene-enhanced plasmonic nanohole arrays for environmental sensing in aqueous samples

2016 ◽  
Vol 7 ◽  
pp. 1564-1573 ◽  
Author(s):  
Christa Genslein ◽  
Peter Hausler ◽  
Eva-Maria Kirchner ◽  
Rudolf Bierl ◽  
Antje J Baeumner ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Binding Capacity ◽  
Gold Films ◽  
Label Free ◽  
Sensor Surface ◽  
Nanohole Array ◽  
Reduced Graphene ◽  
Nanohole Arrays

The label-free nature of surface plasmon resonance techniques (SPR) enables a fast, specific, and sensitive analysis of molecular interactions. However, detection of highly diluted concentrations and small molecules is still challenging. It is shown here that in contrast to continuous gold films, gold nanohole arrays can significantly improve the performance of SPR devices in angle-dependent measurement mode, as a signal amplification arises from localized surface plasmons at the nanostructures. This leads consequently to an increased sensing capability of molecules bound to the nanohole array surface. Furthermore, a reduced graphene oxide (rGO) sensor surface was layered over the nanohole array. Reduced graphene oxide is a 2D nanomaterial consisting of sp2-hybridized carbon atoms and is an attractive receptor surface for SPR as it omits any bulk phase and therefore allows fast response times. In fact, it was found that nanohole arrays demonstrated a higher shift in the resonance angle of 250–380% compared to a continuous gold film. At the same time the nanohole array structure as characterized by its diameter-to-periodicity ratio had minimal influence on the binding capacity of the sensor surface. As a simple and environmentally highly relevant model, binding of the plasticizer diethyl phthalate (DEP) via π-stacking was monitored on the rGO gold nanohole array realizing a limit of detection of as low as 20 nM. The concentration-dependent signal change was studied with the best performing rGO-modified nanohole arrays. Compared to continuous gold films a diameter-to-periodicity ratio (D/P) of 0.43 lead to a 12-fold signal enhancement. Finally, the effect of environmental waters on the sensor was evaluated using samples from sea, lake and river waters spiked with analytically relevant amounts of DEP during which significant changes in the SPR signal are observed. It is expected that this concept can be successfully transferred to enhance the sensitivity in SPR sensors.

High Toughness Inorganic Solid Electrolytes via the Use of Reduced Graphene-Oxide

2020 ◽  
Author(s):  
Christos E. Athanasiou ◽  
Mok Yun Jin ◽  
Cristina Ramirez ◽  
Nitin P. Padture ◽  
Brian W. Sheldon
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Solid Electrolytes ◽  
High Toughness ◽  
Reduced Graphene
Sign in / Sign up

Export Citation Format

Share Document