scholarly journals Graphene-enhanced plasmonic nanohole arrays for environmental sensing in aqueous samples

2016 ◽  
Vol 7 ◽  
pp. 1564-1573 ◽  
Author(s):  
Christa Genslein ◽  
Peter Hausler ◽  
Eva-Maria Kirchner ◽  
Rudolf Bierl ◽  
Antje J Baeumner ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Binding Capacity ◽  
Gold Films ◽  
Label Free ◽  
Sensor Surface ◽  
Nanohole Array ◽  
Reduced Graphene ◽  
Nanohole Arrays

The label-free nature of surface plasmon resonance techniques (SPR) enables a fast, specific, and sensitive analysis of molecular interactions. However, detection of highly diluted concentrations and small molecules is still challenging. It is shown here that in contrast to continuous gold films, gold nanohole arrays can significantly improve the performance of SPR devices in angle-dependent measurement mode, as a signal amplification arises from localized surface plasmons at the nanostructures. This leads consequently to an increased sensing capability of molecules bound to the nanohole array surface. Furthermore, a reduced graphene oxide (rGO) sensor surface was layered over the nanohole array. Reduced graphene oxide is a 2D nanomaterial consisting of sp2-hybridized carbon atoms and is an attractive receptor surface for SPR as it omits any bulk phase and therefore allows fast response times. In fact, it was found that nanohole arrays demonstrated a higher shift in the resonance angle of 250–380% compared to a continuous gold film. At the same time the nanohole array structure as characterized by its diameter-to-periodicity ratio had minimal influence on the binding capacity of the sensor surface. As a simple and environmentally highly relevant model, binding of the plasticizer diethyl phthalate (DEP) via π-stacking was monitored on the rGO gold nanohole array realizing a limit of detection of as low as 20 nM. The concentration-dependent signal change was studied with the best performing rGO-modified nanohole arrays. Compared to continuous gold films a diameter-to-periodicity ratio (D/P) of 0.43 lead to a 12-fold signal enhancement. Finally, the effect of environmental waters on the sensor was evaluated using samples from sea, lake and river waters spiked with analytically relevant amounts of DEP during which significant changes in the SPR signal are observed. It is expected that this concept can be successfully transferred to enhance the sensitivity in SPR sensors.

scholarly journals A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (5) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

AbstractA label-free biosensor is developed for the determination of plasma-based Aβ1–42 biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s−1. A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ1–40 and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ1–42. Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis.

scholarly journals Label-Free DNA Biosensor Using Modified Reduced Graphene Oxide Platform as a DNA Methylation Assay

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4936 ◽  
Author(s):  
Eliska Sedlackova ◽  
Zuzana Bytesnikova ◽  
Eliska Birgusova ◽  
Pavel Svec ◽  
Amir M. Ashrafi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Clinical Diagnostics ◽  
Label Free ◽  
Free Dna ◽  
Methylation Assay ◽  
Reduced Graphene

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)—gold (AuNPs), silver (AgNPs), and copper (CuNPs)—was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA−1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5′-CCGG-3′) islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U·mL−1. Lastly, this study was enriched with the real serum sample, where a 0.19 U·mL−1 LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

scholarly journals A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing

2021 ◽  
Vol 9 ◽  
Author(s):  
Supakeit Chanarsa ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
One Pot ◽  
Reduced Graphene ◽  
Sensitivity And Selectivity ◽  
Analytical Response ◽  
Electrochemical Immunosensing

A bi-functional material based on silver nanoparticles (AgNPs)-reduced graphene oxide (rGO) composite for both electrode modification and signal generation is successfully synthesized for use in the construction of a label-free electrochemical immunosensor. An AgNPs/rGO nanocomposite is prepared by a one-pot wet chemical process. The AgNPs/rGO composite dispersion is simply cast on a screen-printed carbon electrode (SPCE) to fabricate the electrochemical immunosensor. It possesses a sufficient conductivity/electroreactivity and improves the electrode reactivity of SPCE. Moreover, the material can generate an analytical response due to the formation of immunocomplexes for detection of human immunoglobulin G (IgG), a model biomarker. Based on electrochemical stripping of AgNPs, the material reveals signal amplification without external redox molecules/probes. Under optimized conditions, the square wave voltammetric peak current is responded to the logarithm of IgG concentration in two wide linear ranges from 1 to 50 pg.ml−1 and 0.05 to 50 ng.ml−1, and the limit of detection (LOD) is estimated to be 0.86 pg.ml−1. The proposed immunosensor displays satisfactory sensitivity and selectivity. Importantly, detection of IgG in human serum using the immunosensor shows satisfactory accuracy, suggesting that the immunosensor possesses a huge potential for further development in clinical diagnosis.

scholarly journals Reduced Graphene Oxide Fibre Electrodes for Drug Sensing

Proceedings ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 18
Author(s):  
Sutthima Sriprasertsuk ◽  
John R. Varcoe ◽  
Carol Crean
Keyword(s):  
Cyclic Voltammetry ◽  
Graphene Oxide ◽  
Carbon Fibre ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Linear Range ◽  
Oxidation Peak ◽  
Reduced Graphene ◽  
Fibre Sensor ◽  
Model Drug

Reduced graphene oxide (rGO) fibre electrodes and their ability to sense paracetamol (as model drug) were studied. rGO was electrodeposited onto carbon fibre by two different approaches: potentiostatic deposition and cyclic voltammetry (CV) in the presence of graphene oxide solution. Carbon fibre electrodes coated with rGO (after five CV cycles) could sense paracetamol with an oxidation peak at 0.62 V (vs. Ag/AgCl). The limit of detection of this fibre sensor was found to be 36.3 µM with a linear range of 50–500 µM of paracetamol (R2 = 0.9901).

Label-free electrochemical immunosensor based on gold nanoparticle/polyethyleneimine/reduced graphene oxide nanocomposite for the ultrasensitive detection of cancer biomarker matrix metalloproteinase-1

The Analyst ◽  
2021 ◽  
Author(s):  
Xinke Liu ◽  
Lu-Yin Lin ◽  
Fu-Yen Tseng ◽  
Yu-Cheng Tan ◽  
Jian Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Matrix Metalloproteinase ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Matrix Metalloproteinase 1 ◽  
Reduced Graphene ◽  
P Matrix

Matrix metalloproteinase-1 (MMP-1) is associated with many types of cancers, including oral, colorectal, and brain cancers. This paper describes the fabrication of an MMP-1 immunosensor based on a gold nanoparticle/polyethyleneimine/reduced...

Efficient capture and photothermal ablation of planktonic bacteria and biofilms using reduced graphene oxide–polyethyleneimine flexible nanoheaters

2019 ◽  
Vol 7 (17) ◽  
pp. 2771-2781 ◽  
Author(s):  
Milica Budimir ◽  
Roxana Jijie ◽  
Ran Ye ◽  
Alexandre Barras ◽  
Sorin Melinte ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Gram Positive ◽  
Nanohole Array ◽  
Gram Negative ◽  
Planktonic Bacteria ◽  
Photothermal Ablation ◽  
Reduced Graphene

A flexible nanoheater device, consisting of a Au nanohole array coated with reduced graphene oxide–polyethyleneimine, was applied to capture and eradicate both Gram-positive and Gram negative planktonic bacteria and their biofilms.

Sign in / Sign up

Export Citation Format

Share Document