A rational strategy to develop a boron nitride quantum dot-based molecular logic gate and fluorescent assay of alkaline phosphatase activity

2019 ◽  
Vol 7 (6) ◽  
pp. 897-902 ◽  
Author(s):  
Yaqian Han ◽  
Yusheng Niu ◽  
Mengli Liu ◽  
Fushang Niu ◽  
Yuanhong Xu
Keyword(s):  
Alkaline Phosphatase ◽  
Boron Nitride ◽  
Quantum Dot ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Logic Gate ◽  
Fluorescent Assay ◽  
Molecular Logic Gate ◽  
Molecular Logic

By comparing the percentage of FL quenching and recovery of the BNQDs, a Fe3+-mediated FL quenching of BNQDs system was rationally designed for efficient ALP assay. Moreover, the aforementioned ensemble was exploited to newly construct a 2D-QD-based INH logic gate.

A real-time fluorescent assay for the detection of alkaline phosphatase activity based on carbon quantum dots

2015 ◽  
Vol 68 ◽  
pp. 675-680 ◽  
Author(s):  
Zhao Sheng Qian ◽  
Lu Jing Chai ◽  
Yuan Yuan Huang ◽  
Cong Tang ◽  
Jia Jia Shen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Alkaline Phosphatase ◽  
Real Time ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Carbon Quantum Dots ◽  
Fluorescent Assay

Fluorescent assay for alkaline phosphatase activity based on graphene oxide integrating with λ exonuclease

2016 ◽  
Vol 81 ◽  
pp. 460-464 ◽  
Author(s):  
Xue-Guo Liu ◽  
Xiao-Jing Xing ◽  
Bo Li ◽  
Yong-Ming Guo ◽  
Ye-Zhen Zhang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Graphene Oxide ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescent Assay

A regenerable fluorescent quantum dot based nanoprobe for zinc(II), and the design of a molecular logic gate

2015 ◽  
Vol 182 (11-12) ◽  
pp. 2009-2017 ◽  
Author(s):  
Chenxia Hao ◽  
Shaopu Liu ◽  
Wanjun Liang ◽  
Dan Li ◽  
Linlin Wang ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Logic Gate ◽  
Molecular Logic Gate ◽  
Molecular Logic

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

A Molecular Logic Gate Enables Super-Resolved Imaging of Intracellular Lipid Droplets

2019 ◽  
Author(s):  
Adam Eördögh ◽  
Carolina Paganini ◽  
Dorothea Pinotsi ◽  
Paolo Arosio ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Single Molecule ◽  
Lipid Droplets ◽  
Neutral Lipids ◽  
Logic Gate ◽  
Living Cells ◽  
Three Dimensions ◽  
Single Molecule Imaging ◽  
Molecular Logic Gate ◽  
Molecular Logic ◽  
Cellular Compartments

<div>Photoactivatable dyes enable single-molecule imaging in biology. Despite progress in the development of new fluorophores and labeling strategies, many cellular compartments remain difficult to image beyond the limit of diffraction in living cells. For example, lipid droplets, which are organelles that contain mostly neutral lipids, have eluded single-molecule imaging. To visualize these challenging subcellular targets, it is necessary to develop new fluorescent molecular devices beyond simple on/off switches. Here, we report a fluorogenic molecular logic gate that can be used to image single molecules associated with lipid droplets with excellent specificity. This probe requires the subsequent action of light, a lipophilic environment and a competent nucleophile to produce a fluorescent product. The combination of these requirements results in a probe that can be used to image the boundary of lipid droplets in three dimensions with resolutions beyond the limit of diffraction. Moreover, this probe enables single-molecule tracking of lipids within and between droplets in living cells.</div>

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