A label-free electrochemical platform for the detection of antibiotics based on cascade enzymatic amplification coupled with a split G-quadruplex DNAzyme

The Analyst ◽  
2019 ◽  
Vol 144 (16) ◽  
pp. 4995-5002 ◽  
Author(s):  
Rufeng Zhang ◽  
Yu Wang ◽  
Xiaonan Qu ◽  
Shasha Li ◽  
Yihan Zhao ◽  
...  
Keyword(s):  
Electrochemical Sensing ◽  
Label Free ◽  
Enzymatic Amplification ◽  
Specificity And Sensitivity ◽  
Sensing Platform ◽  
G Quadruplex

Herein, a split G-quadruplex DNAzyme as a signal reporter was integrated into an electrochemical sensing platform for the detection of antibiotics with specificity and sensitivity.

An electrochemical sensing platform based on ladder-shaped DNA structure and label-free aptamer for ultrasensitive detection of ampicillin

2019 ◽  
Vol 133 ◽  
pp. 230-235 ◽  
Author(s):  
Seyed Mohammad Taghdisi ◽  
Noor Mohammad Danesh ◽  
Morteza Alinezhad Nameghi ◽  
Mohammad Ramezani ◽  
Mona Alibolandi ◽  
...  
Keyword(s):  
Dna Structure ◽  
Electrochemical Sensing ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Sensing Platform

A signal on aptamer-based electrochemical sensing platform using a triple-helix molecular switch

2014 ◽  
Vol 6 (16) ◽  
pp. 6294-6300 ◽  
Author(s):  
Yudan Du ◽  
Yinfei Mao ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Genping Yan ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Triple Helix ◽  
Molecular Switch ◽  
Electrochemical Sensing ◽  
Label Free ◽  
Sensing Platform ◽  
Aptamer Sequence

A signal on aptamer-based electrochemical sensing platform was demonstrated by taking advantage of a triple-helix molecular switch, which contained a label-free target specific aptamer sequence and a hairpin-shaped structured signal transduction probe.

A label-free fluorescent aptasensor based on HCR and G-quadruplex DNAzymes for the detection of prostate-specific antigen

The Analyst ◽  
2021 ◽  
Author(s):  
Ruirui Zhao ◽  
Lu Zhao ◽  
Haidi Feng ◽  
Xiaoliang Chen ◽  
Huilin Zhang ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Label Free ◽  
Fluorescence Sensing ◽  
Sensing Platforms ◽  
G Quadruplex ◽  
Fluorescent Aptasensor

Fluorescence sensing platforms based on HCR and G-quadruplex DNAzyme amplification strategies for the detection of prostate-specific antigen.

scholarly journals Paper-Based Competitive Immunochromatography Coupled with an Enzyme-Modified Electrode to Enable the Wireless Monitoring and Electrochemical Sensing of Cotinine in Urine

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1659
Author(s):  
Nutcha Larpant ◽  
Pramod K. Kalambate ◽  
Tautgirdas Ruzgas ◽  
Wanida Laiwattanapaisal
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Near Field ◽  
Electrochemical Measurement ◽  
Electrochemical Sensing ◽  
Urine Samples ◽  
Lateral Flow Strip ◽  
Wireless Monitoring ◽  
Sensing Platform ◽  
Combined Strategy

This paper proposes a combined strategy of using paper-based competitive immunochromatography and a near field communication (NFC) tag for wireless cotinine determination. The glucose oxidase labeled cotinine antibody specifically binds free cotinine in a sample, whereas the unoccupied antibody attached to BSA-cotinine at the test line on a lateral flow strip. The glucose oxidase on the strip and an assistant pad in the presence of glucose generated H2O2 and imposed the Ag oxidation on the modified electrode. This enabled monitoring of immunoreaction by either electrochemical measurement or wireless detection. Wireless sensing was realized for cotinine in the range of 100–1000 ng/mL (R2 = 0.96) in PBS medium. Undiluted urine samples from non-smokers exhibited an Ag-oxidation rate three times higher than the smoker’s urine samples. For 1:8 diluted urine samples (smokers), the proposed paper-based competitive immunochromatography coupled with an enzyme-modified electrode differentiated positive and negative samples and exhibited cotinine discrimination at levels higher than 12 ng/mL. This novel sensing platform can potentially be combined with a smartphone as a reader unit.

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