Ultrasensitive detection of transcription factors with a highly-efficient diaminoterephthalate fluorophore via an electrogenerated chemiluminescence strategy

2019 ◽  
Vol 55 (79) ◽  
pp. 11892-11895 ◽  
Author(s):  
Zhenqiang Fan ◽  
Jianfeng Wang ◽  
Nan Hao ◽  
Yihao Li ◽  
Yuhang Yin ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Electrogenerated Chemiluminescence ◽  
Ultrasensitive Detection ◽  
Sensitive Assay ◽  
Hairpin Dna ◽  
Highly Efficient ◽  
Amplification Strategy

Herein, we apply electrogenerated chemiluminescence (ECL) based method employing diaminoterephthalate analogue as ECL emitter and hairpin DNA as amplification strategy, for sensitive assay of transcription factors.

Hairpin DNA-fueled dynamic self-assembly of three-arm DNA branched junctions consisting of active DNAzyme structures for enzyme-free ultrasensitive detection of nucleic acids

2016 ◽  
Vol 8 (47) ◽  
pp. 8262-8265 ◽  
Author(s):  
Ke Yang ◽  
Ming Zeng ◽  
Xing He ◽  
Jianming Li ◽  
Dinggeng He
Keyword(s):  
Nucleic Acids ◽  
Self Assembly ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Hairpin Dna ◽  
Amplification Strategy ◽  
Dual Signal Amplification

An enzyme-free dual signal amplification strategy based on programmable molecular hairpins has been developed for amplified detection of DNA via the hairpin DNA-fueled dynamic self-assembly of three-arm DNAzyme.

Electrogenerated chemiluminescence aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe

Talanta ◽  
2014 ◽  
Vol 130 ◽  
pp. 370-376 ◽  
Author(s):  
Zhejian Li ◽  
Lijuan Sun ◽  
Ying Zhao ◽  
Libin Yang ◽  
Honglan Qi ◽  
...  
Keyword(s):  
Electrogenerated Chemiluminescence ◽  
Ultrasensitive Detection

Upconversional Nanoprobes with Highly Efficient Energy Transfer for Ultrasensitive Detection of Alkaline Phosphatase

ACS Sensors ◽  
2019 ◽  
Vol 4 (11) ◽  
pp. 2864-2868 ◽  
Author(s):  
Mengping Gao ◽  
Ruiying Wu ◽  
Qingsong Mei ◽  
Cuilan Zhang ◽  
Xiao Ling ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Energy Transfer ◽  
Ultrasensitive Detection ◽  
Efficient Energy Transfer ◽  
Efficient Energy ◽  
Highly Efficient

scholarly journals ProSampler: an ultrafast and accurate motif finder in large ChIP-seq datasets for combinatory motif discovery

2019 ◽  
Vol 35 (22) ◽  
pp. 4632-4639 ◽  
Author(s):  
Yang Li ◽  
Pengyu Ni ◽  
Shaoqiang Zhang ◽  
Guojun Li ◽  
Zhengchang Su
Keyword(s):  
Transcription Factors ◽  
Gibbs Sampler ◽  
Binding Sites ◽  
Motif Discovery ◽  
Source Code ◽  
Motif Finding ◽  
Supplementary Information ◽  
Highly Efficient ◽  
Motif Finder ◽  
Motif Finding Algorithm

Abstract Motivation The availability of numerous ChIP-seq datasets for transcription factors (TF) has provided an unprecedented opportunity to identify all TF binding sites in genomes. However, the progress has been hindered by the lack of a highly efficient and accurate tool to find not only the target motifs, but also cooperative motifs in very big datasets. Results We herein present an ultrafast and accurate motif-finding algorithm, ProSampler, based on a novel numeration method and Gibbs sampler. ProSampler runs orders of magnitude faster than the fastest existing tools while often more accurately identifying motifs of both the target TFs and cooperators. Thus, ProSampler can greatly facilitate the efforts to identify the entire cis-regulatory code in genomes. Availability and implementation Source code and binaries are freely available for download at https://github.com/zhengchangsulab/prosampler. It was implemented in C++ and supported on Linux, macOS and MS Windows platforms. Supplementary information Supplementary materials are available at Bioinformatics online.

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