PLGA–collagen–ECM hybrid scaffolds functionalized with biomimetic extracellular matrices secreted by mesenchymal stem cells during stepwise osteogenesis-co-adipogenesis

2019 ◽  
Vol 7 (45) ◽  
pp. 7195-7206 ◽  
Author(s):  
Yazhou Chen ◽  
Kyubae Lee ◽  
Naoki Kawazoe ◽  
Yingnan Yang ◽  
Guoping Chen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
3D Model ◽  
Extracellular Matrices ◽  
Hybrid Scaffolds

Development of an in vitro 3D model that reflects the dynamic remodeling of ECMs during simultaneous osteogenesis and adipogenesis of hMSCs.

In vitro and in vivo osteogenic potential of bioactive glass–PVA hybrid scaffolds colonized by mesenchymal stem cells

2012 ◽  
Vol 7 (1) ◽  
pp. 015004 ◽  
Author(s):  
Viviane S Gomide ◽  
Alessandra Zonari ◽  
Natalia M Ocarino ◽  
Alfredo M Goes ◽  
Rogéria Serakides ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioactive Glass ◽  
Osteogenic Potential ◽  
Hybrid Scaffolds

scholarly journals Hybrid Sponge-Like Scaffolds Based on Ulvan and Gelatin: Design, Characterization and Evaluation of Their Potential Use in Bone Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1763
Author(s):  
Leto-Aikaterini Tziveleka ◽  
Andreas Sapalidis ◽  
Stefanos Kikionis ◽  
Eleni Aggelidou ◽  
Efterpi Demiri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Performance ◽  
Cell Attachment ◽  
Sulfated Polysaccharide ◽  
Hybrid Scaffolds

Ulvan, a bioactive natural sulfated polysaccharide, and gelatin, a collagen-derived biopolymer, have attracted interest for the preparation of biomaterials for different biomedical applications, due to their demonstrated compatibility for cell attachment and proliferation. Both ulvan and gelatin have exhibited osteoinductive potential, either alone or in combination with other materials. In the current work, a series of novel hybrid scaffolds based on crosslinked ulvan and gelatin was designed, prepared and characterized. Their mechanical performance, thermal stability, porosity, water-uptake and in vitro degradation ability were assessed, while their morphology was analyzed through scanning electron microscopy. The prepared hybrid ulvan/gelatin scaffolds were characterized by a highly porous and interconnected structure. Human adipose-derived mesenchymal stem cells (hADMSCs) were seeded in selected ulvan/gelatin hybrid scaffolds and their adhesion, survival, proliferation, and osteogenic differentiation efficiency was evaluated. Overall, it was found that the prepared hybrid sponge-like scaffolds could efficiently support mesenchymal stem cells’ adhesion and proliferation, suggesting that such scaffolds could have potential uses in bone tissue engineering.

scholarly journals Studying the induction effect of different mouse neck scaffolds on the behavior of Mouse Bone marrow-derived mesenchymal stem cell

2021 ◽  
Vol 15 (6) ◽  
pp. 1790-1799
Author(s):  
Mohamadkazem Ganjavi ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Nasser Mahdavi- Shahri
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Sodium Dodecyl ◽  
Extracellular Matrices ◽  
Mouse Bone Marrow ◽  
Induction Effect ◽  
Histological Studies ◽  
Neck Area

Objective: Studying the behavior of mesenchymal stem cells is important for understanding some physiological and pathological processes as well as long-term preservation of these cells in vitro. The neck area of mouse contains a wide variety of different organs and tissues and extracellular matrices. In the present study, the interaction of mouse bone marrow derived mesenchymal stem cells (BM-MSCs) with mouse neck scaffolds was performed in order to have a better understanding of the behavior of mesenchymal stem cells. Materials and methods: The neck areas of 9 two-week-old Syrian mice were cut into pieces with 4 mm thickness. Decellularization process was carried through snap freeze-thaw cycles followed by sodium dodecyl sulfate (SDS) treatments. After confirmation of acellularization through histological studies and DNA extraction process, scaffolds were co-cultured with mouse (BM-MSCs) up to 21 days, in vitro. Results: As indicated by histological studies and scanning of electron microscopy, stem cells migrate into the cartilage scaffold on third, seventh and fourteenth days of culture. Stem cells were divided on these days. On the fourteenth day of culture, migration to epidermal and blood vascular scaffolds was also observed. On the twenty-first day of culture, cells were observed only in the cartilage scaffold, while showing morphological differentiation. Conclusion: As indicated by the results of the present study, mouse neck scaffold, due to its diverse extracellular matrices, is a suitable structure for studying some cellular behaviors such as migration, cell division and cell differentiation. However, more molecular studies are needed to determine the extent of this differentiation. Keywords: Natural scaffold, Cell behaviors, Cell-matrix interaction, Experimental model scaffolding, mouse neck area

Autologous transplantation of mesenchymal stem cells into the portal vein of the miniature pig; a preliminary experiment for NOTES approach

2019 ◽  
Vol 98 (9) ◽  
pp. 350-355
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Portal Vein ◽  
Liver Parenchyma ◽  
Miniature Pig ◽  
Hepatic Diseases ◽  
Study Results

Introduction: There is evidence that mesenchymal stem cells (MSCs) could trans-differentiate into the liver cells in vitro and in vivo and thus may be used as an unfailing source for stem cell therapy of liver disease. Combination of MSCs (with or without their differentiation in vitro) and minimally invasive procedures as laparoscopy or Natural Orifice Transluminal Endoscopic Surgery (NOTES) represents a chance for many patients waiting for liver transplantation in vain. Methods: Over 30 millions of autologous MSCs at passage 3 were transplanted via the portal vein in an eight months old miniature pig. The deposition of transplanted cells in liver parenchyma was evaluated histologically and the trans-differential potential of CM-DiI labeled cells was assessed by expression of pig albumin using immunofluorescence. Results: Three weeks after transplantation we detected the labeled cells (solitary, small clusters) in all 10 samples (2 samples from each lobe) but no diffuse distribution in the samples. The localization of CM-DiI+ cells was predominantly observed around the portal triads. We also detected the localization of albumin signal in CM-DiI labeled cells. Conclusion: The study results showed that the autologous MSCs (without additional hepatic differentiation in vitro) transplantation through the portal vein led to successful infiltration of intact miniature pig liver parenchyma with detectable in vivo trans-differentiation. NOTES as well as other newly developed surgical approaches in combination with cell therapy seem to be very promising for the treatment of hepatic diseases in near future.

In vitro effect of prolactin on the osteogenic potential of bone marrow mesenchymal stem cells of rats

2013 ◽  
Author(s):  
Melo Ocarino Natalia de ◽  
Silvia Silva Santos ◽  
Lorena Rocha ◽  
Juneo Freitas ◽  
Reis Amanda Maria Sena ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Effect

Characterization of mesenchymal stem cells and their application in experimental embryology

2013 ◽  
Vol 16 (3) ◽  
pp. 593-599 ◽  
Author(s):  
J. Opiela ◽  
M. Samiec
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Somatic Cell ◽  
Fertilization In Vitro ◽  
Cell Cloning ◽  
Mammalian Embryo ◽  
Vitro Fertilization ◽  
Experimental Embryology ◽  
Genomic Engineering

Abstract The efficiency of somatic cell cloning (somatic cell nuclear transfer; SCNT) as well as in vitro fertilization/in vitro embryo production (IVF/IVP) in mammals stay at relatively same level for over a decade. Despite plenty of different approaches none satisfactory break-through took place. In this article, we briefly summarize the implementation of mesenchymal stem cells (MSCs) for experimental embryology. The advantages of using MSCs as nuclear donors in somatic cell cloning and in vitro embryo culture are described. The description of results obtained with these cells in mammalian embryo genomic engineering is presented.

Sign in / Sign up

Export Citation Format

Share Document