A label-free electrochemical sensor for ultrasensitive microRNA-21 analysis based on poly(l-cysteine)/MoS2 sensing interface

The Analyst ◽  
2021 ◽  
Author(s):  
Yao Yao ◽  
Hemeng Pan ◽  
Yi Luo ◽  
Dan Zhu ◽  
Jie Chao ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Electrochemical Sensor ◽  
Molybdenum Disulfide ◽  
Label Free ◽  
Label Free Detection

Label-free detection of nucleic acids attracts scientists’ interests due to its simple, fast and efficient. Herein, l-cysteine was electropolymerized on molybdenum disulfide (MoS2) surface to form a stable and electroactive...

Biomimetic nanochannels based biosensor for ultrasensitive and label-free detection of nucleic acids

2016 ◽  
Vol 86 ◽  
pp. 194-201 ◽  
Author(s):  
Zhongyue Sun ◽  
Tangbin Liao ◽  
Yulin Zhang ◽  
Jing Shu ◽  
Hong Zhang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Label Free ◽  
Label Free Detection

Disposable paper-based electrochemical sensor using thiol-terminated poly(2-methacryloyloxyethyl phosphorylcholine) for the label-free detection of C-reactive protein

2019 ◽  
Vol 186 (7) ◽  
Author(s):  
Chanika Pinyorospathum ◽  
Sudkate Chaiyo ◽  
Pornpen Sae-ung ◽  
Voravee P. Hoven ◽  
Panittha Damsongsang ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Label Free Detection

Label free detection of nucleic acids by modulating nanochannel surfaces

2015 ◽  
Vol 51 (12) ◽  
pp. 2335-2338 ◽  
Author(s):  
Pete Crisalli ◽  
Christopher McCallum ◽  
Sumita Pennathur
Keyword(s):  
Nucleic Acids ◽  
Label Free ◽  
Surface Charges ◽  
Nanofluidic Channels ◽  
Label Free Detection ◽  
Electronic Detection

Modulating surface charges of nanofluidic channels allows for label-free all-electronic detection of both specific and non-specific nucleic acids.

scholarly journals Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3137
Author(s):  
Yen-Ta Tseng ◽  
Wan-Yun Li ◽  
Ya-Wen Yu ◽  
Chang-Yue Chiang ◽  
Su-Qin Liu ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
Nucleic Acids ◽  
Real Time ◽  
Plasmon Resonance ◽  
Fiber Optic ◽  
Hla B27 ◽  
Label Free ◽  
Mrna Detection ◽  
Label Free Detection ◽  
Particle Plasmon

We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-μL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance.

Label-free detection of nucleic acids by turn-on and turn-off G-quadruplex-mediated fluorescence

2011 ◽  
Vol 399 (8) ◽  
pp. 2763-2770 ◽  
Author(s):  
Jiangtao Ren ◽  
Haixia Qin ◽  
Jiahai Wang ◽  
Nathan W. Luedtke ◽  
Erkang Wang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Label Free ◽  
Turn On ◽  
Label Free Detection ◽  
G Quadruplex

Highly sensitive electrochemical detection of circulating tumor DNA based on thin-layer MoS2/graphene composites

RSC Advances ◽  
2016 ◽  
Vol 6 (27) ◽  
pp. 22673-22678 ◽  
Author(s):  
Yilan Chu ◽  
Bin Cai ◽  
Ye Ma ◽  
Minggang Zhao ◽  
Zhizhen Ye ◽  
...  
Keyword(s):  
Thin Layer ◽  
Electrochemical Sensor ◽  
Molybdenum Disulfide ◽  
Electrochemical Detection ◽  
Ultrasonic Method ◽  
Hydrothermal Process ◽  
Circulating Tumor Dna ◽  
Label Free ◽  
Highly Sensitive ◽  
Tumor Dna

By integrating thin-layer molybdenum disulfide (MoS2) and graphene through a hydrothermal process and an ultrasonic method, a label-free, amplification-free and ultrasensitive circulating tumor DNA electrochemical sensor was made.

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