scholarly journals Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3137
Author(s):  
Yen-Ta Tseng ◽  
Wan-Yun Li ◽  
Ya-Wen Yu ◽  
Chang-Yue Chiang ◽  
Su-Qin Liu ◽  
...  

We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-μL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance.

2013 ◽  
Vol 41 ◽  
pp. 268-274 ◽  
Author(s):  
Hsing-Ying Lin ◽  
Chen-Han Huang ◽  
Shin-Huei Chen ◽  
Yu-Chia Liu ◽  
Wei-Zhe Chang ◽  
...  

Talanta ◽  
2016 ◽  
Vol 146 ◽  
pp. 291-298 ◽  
Author(s):  
Chin-Wei Wu ◽  
Chang-Yue Chiang ◽  
Chien-Hsing Chen ◽  
Chung-Sheng Chiang ◽  
Chih-To Wang ◽  
...  

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3411-3420 ◽  
Author(s):  
Yijia Wang ◽  
Shiwu Zhang ◽  
Chunze Zhang ◽  
Zhenying Zhao ◽  
Xiaoli Zheng ◽  
...  

The real-time and label free detection abilities of surface plasmon resonance (SPR) biosensors provide a way of evaluating the influence of some genes’ expression on anti-tumor drug cytotoxicity.


2012 ◽  
Author(s):  
Hsing-Ying Lin ◽  
Chen-Han Huang ◽  
Yu-Chia Liu ◽  
Shin-Huei Chen ◽  
Lai-Kwan Chau

RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 23990-23998 ◽  
Author(s):  
Gaoling Liang ◽  
Zhongjun Zhao ◽  
Yin Wei ◽  
Kunping Liu ◽  
Wenqian Hou ◽  
...  

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).


ACS Sensors ◽  
2021 ◽  
Author(s):  
Pallavi Halkare ◽  
Nirmal Punjabi ◽  
Jigme Wangchuk ◽  
Santhosh Madugula ◽  
Kiran Kondabagil ◽  
...  

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