Dissolvable Mg/Al layered double hydroxides and surfactant as an extractant for trace analysis of benzoylurea insecticides by high performance liquid chromatography

A simple and rapid preconcentration method using dissolvable Mg/Al LDHs and SDS has been demonstrated for high performance liquid chromatographic analysis of benzoylurea insecticides in water and honey samples.

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Application of "high-performance" liquid chromatography to the study of sphingolipidoses.

Clinical Chemistry ◽

10.1093/clinchem/26.10.1499 ◽

1980 ◽

Vol 26 (10) ◽

pp. 1499-1503 ◽

Cited By ~ 15

Author(s):

M D Ullman ◽

R E Pyeritz ◽

H W Moser ◽

D A Wenger ◽

E H Kolodny

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Plasma Exchange ◽

Chromatographic Analysis ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Urine Sediment ◽

Liquid Chromatographic Analysis ◽

Liquid Chromatographic

Abstract Quantitative high-performance liquid chromatographic analysis of perbenzoylated sphingolipids has been used to study the correlations of body chemistry to clinical phenomena. Plasma sphingolipids were isolated from 32 Gaucher (β-glucosidase deficiency) and six Fabry (α-galactosidase deficiency) patients by solvent partition and chromatographic separation on silicic acid columns. Plasma sphingolipids from a patient undergoing plasma-exchange were separated from interfering lipids with reversed-phase columns. Liquid-chromatographic analysis of sphingolipids provides useful supportive information for diagnoses because affected individuals are shown to possess increased circulating concentrations of the pathognomonic sphingolipid. We also used this technique to monitor sphingolipid concentrations in plasma and urine sediment during plasma exchange of a p atient with Fabry’s disease. Regular plasma exchanges produced and maintained decreased concentrations of sphingolipids in plasma, but near pre-exchange concentrations were observed within days after the therapy was terminated.

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Sensitive analysis of ethanolamine- and serine-containing phosphoglycerides by high-performance liquid chromatography

Biochemical Journal ◽

10.1042/bj1450517 ◽

1975 ◽

Vol 145 (3) ◽

pp. 517-526 ◽

Cited By ~ 67

Author(s):

F B Jungalwala ◽

R J Turel ◽

J E Evans ◽

R H McCluer

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Limit Of Detection ◽

High Performance Liquid Chromatographic ◽

Amino Groups ◽

Tissue Samples ◽

Primary Amino ◽

Liquid Chromatographic Analysis ◽

Liquid Chromatographic

A highly sensitive method for the separation and quantitative measurement of phospholipids containing primary amino groups, such as phosphatidylethanolamine, phosphatidylserine and lysophosphatidylethanolamine, is described. The method involves a simple and quantitative derivative formation of the phospholipids containing amino groups to their u.v.-absorbing biphenylcarbonyl derivatives. These have molar extinction coefficients of about 23,000 at 268nm. The phospholipid derivatives are then separated and non-destructively determined by high-performance liquid chromatography. The amino phospholipids containing vinyl ether bonds (plasmalogens) can be determined separately from the diacyl- and alkylacyl-amino phospholipids. The lower limit of detection by high-performance liquid-chromatographic analysis of the phospholipid derivatives is about 10-13pmol or 0.3-0.4ng of phospholipid P. The quantitative range of derivative formation and analysis by high-performance liquid chromatography of the phospholipids containing amino groups was shown to be 10-500nmol. The method was shown to be applicable to the analysis of phospholipids containing amino groups in tissue samples.

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High-performance liquid chromatography of phosphatidylcholine and sphingomyelin with detection in the region of 200 nm

Biochemical Journal ◽

10.1042/bj1550055 ◽

1976 ◽

Vol 155 (1) ◽

pp. 55-60 ◽

Cited By ~ 163

Author(s):

F B Jungalwala ◽

J E Evans ◽

R H McCluer

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Animal Tissues ◽

Degree Of Unsaturation ◽

Liquid Chromatographic Method ◽

Absorbing Material ◽

Liquid Chromatographic Analysis ◽

Liquid Chromatographic

A sensitive method for the separation of phosphatidylcholine and sphingomyelin by high-performance liquid chromatographic analysis is described. The elution of the phospholipids from a microparticulate (10 mum) silica-gel chromatographic column was monitored with an ultraviolet spectromonitor at 203 nm. Acetonitrile/methanol/water (65:21:14, by vol.) was used as the solvent. It was shown by using synthetic phosphatidylcholines of knowm fatty acid composition and of varying degree of unsaturation that the absorption at 203 nm was primarily due to the isolated double bonds and the response measured varied with the degree of unsaturation. Approx. 1 nmol of phosphatidylcholine, containing at least one double bond per molecule, can be detected. The amounts of phosphatidylcholine and sphingomyelin could be determined by high-performance liquid chromatography and ultraviolet absorption if the apparent extinction coefficient of the material analyzed was established. Alternatively, peaks were collected and the phospholipids were determined by the analysis of phosphorus. The analysis of phosphatidylcholine and sphingomyelin present in the lipid extracts from animal tissues, blood and amniotic fluids were made without interference from other phospholipids or ultraviolet-absorbing material. The method described here is complementary to the high-performance liquid chromatographic method described previously for the analysis of ethanolamine-containing phosphoglycerides and serine-containing phosphoglycerides [Jungalwala, Turel, Evans and McCluer (1975) Biochem. J. 145, 517-526].

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