A general separation method of phenolic acids using pH-zone-refining counter-current chromatography and its application to oat bran

A separation method using pH-ZRCCC combined with HSCCC was proposed to separate the crude sample of Perillae Folium with different constituents and polarity, and three phenolic acids and four flavonoid glycosides were isolated successfully.

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Preparative separation of seven phenolic acids from Xanthii Fructus using pH-zone-refining counter-current chromatography combined with semi-preparative high performance liquid chromatography

RSC Advances ◽

10.1039/c9ra06969k ◽

2019 ◽

Vol 9 (63) ◽

pp. 36524-36529

Author(s):

Tianyu Ma ◽

Lihua Xu ◽

Xinming Wang ◽

Jia Li ◽

Lanping Guo ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Caffeic Acid ◽

Phenolic Acids ◽

High Performance ◽

Preparative Hplc ◽

Zone Refining ◽

Preparative Separation ◽

Caffeoylquinic Acid ◽

Counter Current

Segmentation and purification strategy using pH-zone-refining counter-current chromatography combined with semi-preparative HPLC for separation of six caffeoylquinic acid isomers and a caffeic acid from Xanthii Fructus.

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Isolation and Purification of Phenolic Acids from Sugarcane (Saccharum officinarum L.) Rinds by pH-Zone-Refining Counter-Current Chromatography and Their Antioxidant Activity Evaluation

Food Analytical Methods ◽

10.1007/s12161-017-0824-3 ◽

2017 ◽

Vol 10 (7) ◽

pp. 2576-2584 ◽

Cited By ~ 2

Author(s):

Ying-Tong Fang ◽

Quan Li ◽

Ao-Cheng Cao ◽

Yuan Li ◽

Yun Wei

Keyword(s):

Antioxidant Activity ◽

Phenolic Acids ◽

Saccharum Officinarum ◽

Zone Refining ◽

Isolation And Purification ◽

Activity Evaluation ◽

Counter Current

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One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

The Natural Products Journal ◽

10.2174/2210315508666181004115422 ◽

2019 ◽

Vol 9 (2) ◽

pp. 138-143

Author(s):

Tianyun Li ◽

Xiling Dai ◽

Yichen Li ◽

Guozheng Huang ◽

Jianguo Cao

Keyword(s):

Natural Products ◽

Liquid Chromatography ◽

Phenolic Acids ◽

High Speed ◽

High Efficiency ◽

Total Phenolic ◽

Medium Pressure ◽

Medium Pressure Liquid Chromatography ◽

One Step ◽

Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

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