scholarly journals Substrate stiffness induced mechanotransduction regulates temporal evolution of human fetal neural progenitor cell phenotype, differentiation, and biomechanics

2020 ◽  
Vol 8 (19) ◽  
pp. 5452-5464
Author(s):  
Chandrasekhar Kothapalli ◽  
Gautam Mahajan ◽  
Kurt Farrell
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Progenitor Cell ◽  
Temporal Evolution ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Substrate Stiffness ◽  
Cell Phenotype ◽  
Spatiotemporal Evolution

We here report on the substrate stiffness dependent spatiotemporal evolution of mechanical properties of neural stem cells and their progenies.

scholarly journals An efficient platform for astrocyte differentiation from human induced pluripotent stem cells

2017 ◽  
Author(s):  
TCW Julia ◽  
Minghui Wang ◽  
Anna A. Pimenova ◽  
Kathryn R. Bowles ◽  
Brigham J. Hartley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Progenitor Cell ◽  
Induced Pluripotent Stem Cell ◽  
Calcium Transient ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Phagocytic Capacity ◽  
Induced Pluripotent

SUMMARYGrowing evidence implicates the importance of glia, particularly astrocytes, in neurological and psychiatric diseases. Here, we describe a rapid and robust method for the differentiation of highly pure populations of replicative astrocytes from human induced pluripotent stem cells (hiPSCs), via a neural progenitor cell (NPC) intermediate. Using this method, we generated hiPSC-derived astrocyte populations (hiPSC-astrocytes) from 42 NPC lines (derived from 30 individuals) with an average of ∼90% S100β-positive cells. Transcriptomic analysis demonstrated that the hiPSC-astrocytes are highly similar to primary human fetal astrocytes and characteristic of a non-reactive state. hiPSC-astrocytes respond to inflammatory stimulants, display phagocytic capacity and enhance microglial phagocytosis. hiPSC-astrocytes also possess spontaneous calcium transient activity. Our novel protocol is a reproducible, straightforward (single media) and rapid (<30 days) method to generate homogenous populations of hiPSC-astrocytes that can be used for neuron-astrocyte and microglia-astrocyte co-cultures for the study of neuropsychiatric disorders.ABBREVIATIONShiPSChuman induced pluripotent stem cellNPCneural progenitor cell

Exosomes derived from Wharton's jelly stem cells switch neural progenitor cell fate to oligodendrogenesis

2018 ◽  
Author(s):  
M Joerger-Messerli ◽  
M Spinelli ◽  
G Thomi ◽  
V Haesler ◽  
M Müller ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Fate ◽  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Wharton’S Jelly ◽  
Wharton's Jelly

The effects of hyaluronic acid hydrogels with tunable mechanical properties on neural progenitor cell differentiation

Biomaterials ◽  
2010 ◽  
Vol 31 (14) ◽  
pp. 3930-3940 ◽  
Author(s):  
Stephanie K. Seidlits ◽  
Zin Z. Khaing ◽  
Rebecca R. Petersen ◽  
Jonathan D. Nickels ◽  
Jennifer E. Vanscoy ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Hyaluronic Acid ◽  
Cell Differentiation ◽  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Tunable Mechanical Properties

scholarly journals Glucocorticoids promote neural progenitor cell proliferation derived from human induced pluripotent stem cells

SpringerPlus ◽  
2014 ◽  
Vol 3 (1) ◽  
pp. 527 ◽  
Author(s):  
Eiichi Ninomiya ◽  
Taeka Hattori ◽  
Masashi Toyoda ◽  
Akihiro Umezawa ◽  
Takashi Hamazaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Progenitor Cell Proliferation ◽  
Induced Pluripotent

scholarly journals Large-scale generation of human iPSC-derived neural stem cells/early neural progenitor cells and their neuronal differentiation

Organogenesis ◽  
2014 ◽  
Vol 10 (4) ◽  
pp. 365-377 ◽  
Author(s):  
Leonardo D’Aiuto ◽  
Yun Zhi ◽  
Dhanjit Kumar Das ◽  
Madeleine R Wilcox ◽  
Jon W Johnson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Progenitor Cells ◽  
Neuronal Differentiation ◽  
Large Scale ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Human Ipsc

scholarly journals Histone chaperone HIRA regulates neural progenitor cell proliferation and neurogenesis via β-catenin

2017 ◽  
Vol 216 (7) ◽  
pp. 1975-1992 ◽  
Author(s):  
Yanxin Li ◽  
Jianwei Jiao
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Progenitor Cell ◽  
Neural Progenitor Cells ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Histone Chaperone ◽  
Epigenetic Mechanism ◽  
Progenitor Cell Proliferation ◽  
Early Neurogenesis

Histone cell cycle regulator (HIRA) is a histone chaperone and has been identified as an epigenetic regulator. Subsequent studies have provided evidence that HIRA plays key roles in embryonic development, but its function during early neurogenesis remains unknown. Here, we demonstrate that HIRA is enriched in neural progenitor cells, and HIRA knockdown reduces neural progenitor cell proliferation, increases terminal mitosis and cell cycle exit, and ultimately results in premature neuronal differentiation. Additionally, we demonstrate that HIRA enhances β-catenin expression by recruiting H3K4 trimethyltransferase Setd1A, which increases H3K4me3 levels and heightens the promoter activity of β-catenin. Significantly, overexpression of HIRA, HIRA N-terminal domain, or β-catenin can override neurogenesis abnormities caused by HIRA defects. Collectively, these data implicate that HIRA, cooperating with Setd1A, modulates β-catenin expression and then regulates neurogenesis. This finding represents a novel epigenetic mechanism underlying the histone code and has profound and lasting implications for diseases and neurobiology.

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