scholarly journals Redox switching of an artificial transmembrane signal transduction system

2021 ◽  
Vol 57 (17) ◽  
pp. 2196-2198
Author(s):  
Lucia Trevisan ◽  
Istvan Kocsis ◽  
Christopher A. Hunter
Keyword(s):  
Signal Transduction ◽  
Ascorbic Acid ◽  
Membrane Translocation ◽  
Signal Transduction System ◽  
System P ◽  
Redox Switching

An external redox signal delivered by ascorbic acid was used to trigger membrane translocation in a synthetic transduction system.

A novel label-free fluorescence strategy for methyltransferase activity assay based on dsDNA-templated copper nanoparticles coupled with an endonuclease-assisted signal transduction system

The Analyst ◽  
2016 ◽  
Vol 141 (4) ◽  
pp. 1383-1389 ◽  
Author(s):  
Q. Q. Lai ◽  
M. D. Liu ◽  
C. C. Gu ◽  
H. G. Nie ◽  
X. J. Xu ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Copper Nanoparticles ◽  
Fluorescence Method ◽  
Label Free ◽  
Activity Assay ◽  
Signal Transduction System ◽  
Methyltransferase Activity ◽  
System P

A novel label-free fluorescence method by coupling dsDNA-templated CuNPs with endonuclease-assisted signal transduction has been developed for methyltransferase activity assay.

scholarly journals Reconfiguration of signal transduction system with synthetic ligand FK1012.

1997 ◽  
Vol 35 (9) ◽  
pp. 641-642
Author(s):  
SACHIKO MACHIDA
Keyword(s):  
Signal Transduction ◽  
Signal Transduction System ◽  
Synthetic Ligand

scholarly journals Dissection of a Hypoxia-induced, Nitric Oxide–mediated Signaling Cascade

2009 ◽  
Vol 20 (18) ◽  
pp. 4083-4090 ◽  
Author(s):  
Pascale F. Dijkers ◽  
Patrick H. O'Farrell
Keyword(s):  
Nitric Oxide ◽  
Signal Transduction ◽  
Signaling Cascade ◽  
S2 Cells ◽  
Signal Transduction System ◽  
Signaling Systems ◽  
Calcium Dependent ◽  
Drosophila S2 Cells ◽  
Oxide Synthase ◽  
Multiple Signaling

Befitting oxygen's key role in life's processes, hypoxia engages multiple signaling systems that evoke pervasive adaptations. Using surrogate genetics in a powerful biological model, we dissect a poorly understood hypoxia-sensing and signal transduction system. Hypoxia triggers NO-dependent accumulation of cyclic GMP and translocation of cytoplasmic GFP-Relish (an NFκB/Rel transcription factor) to the nucleus in Drosophila S2 cells. An enzyme capable of eliminating NO interrupted signaling specifically when it was targeted to the mitochondria, arguing for a mitochondrial NO signal. Long pretreatment with an inhibitor of nitric oxide synthase (NOS), L-NAME, blocked signaling. However, addition shortly before hypoxia was without effect, suggesting that signaling is supported by the prior action of NOS and is independent of NOS action during hypoxia. We implicated the glutathione adduct, GSNO, as a signaling mediator by showing that overexpression of the cytoplasmic enzyme catalyzing its destruction, GSNOR, blocks signaling, whereas knockdown of this activity caused reporter translocation in the absence of hypoxia. In downstream steps, cGMP accumulated, and calcium-dependent signaling was subsequently activated via cGMP-dependent channels. These findings reveal the use of unconventional steps in an NO pathway involved in sensing hypoxia and initiating signaling.

Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system

2018 ◽  
Vol 11 (525) ◽  
pp. eaaq0825 ◽  
Author(s):  
Gareth S. A. Wright ◽  
Akane Saeki ◽  
Takaaki Hikima ◽  
Yoko Nishizono ◽  
Tamao Hisano ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Oxygen Sensing ◽  
Signal Transduction System ◽  
Two Component ◽  
Two Component Signal Transduction

scholarly journals Characterization of changes of pain behavior and signal transduction system in food-deprived mice

2018 ◽  
Vol 22 (4) ◽  
pp. 227-233 ◽  
Author(s):  
Sang-Pil Jang ◽  
Seong-Hwan Park ◽  
Jun-Sub Jung ◽  
Hee-Jung Lee ◽  
Jung-Woo Hong ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Pain Behavior ◽  
Signal Transduction System
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