Cyclometalated iridium(iii) complex nanoparticles for mitochondria-targeted photodynamic therapy

Nanoscale ◽  
2020 ◽  
Vol 12 (26) ◽  
pp. 14061-14067 ◽  
Author(s):  
Huan Lu ◽  
Xinpeng Jiang ◽  
Yanyan Chen ◽  
Ke Peng ◽  
Yiming Huang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
White Light ◽  
Breast Cancer Cells ◽  
Water Solubility ◽  
Targeting Ability ◽  
Targeted Photodynamic Therapy ◽  
Mitochondria Targeting ◽  
Mcf 7

Ir(tiq)2ppy nanoparticles are prepared to achieve water solubility and mitochondria-targeting ability with high PDT efficiency to MCF-7 breast cancer cells under white light irradiation.

Photodynamic therapy activities of phthalocyanine-based macromolecular photosensitizers on MCF-7 breast cancer cells

2021 ◽  
pp. 1-10
Author(s):  
Erem Ahmetali ◽  
Pinar Sen ◽  
N. Ceren Süer ◽  
Tebello Nyokong ◽  
Tarik Eren ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Mcf 7

scholarly journals In vitro combined effect of Doxorubicin and sulfonated zinc Phthalocyanine–mediated photodynamic therapy on MCF-7 breast cancer cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (10) ◽  
pp. 101042831772727 ◽  
Author(s):  
Eric Chekwube Aniogo ◽  
Blassan Plackal Adimuriyil George ◽  
Heidi Abrahamse
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Combined Effect ◽  
Zinc Phthalocyanine ◽  
Mcf 7

scholarly journals Photodynamic activity of Sn(IV) meso-tetraacenaphthylporphyrin and its methyl-β-cyclodextrin inclusion complexes on MCF-7 breast cancer cells

2019 ◽  
Vol 23 (11n12) ◽  
pp. 1486-1494
Author(s):  
Nthabeleng Molupe ◽  
Balaji Babu ◽  
Earl Prinsloo ◽  
Abdessamad Y. A. Kaassis ◽  
Katharina Edkins ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Water Solubility ◽  
Therapeutic Window ◽  
Cyclodextrin Inclusion Complexes ◽  
Cyclodextrin Inclusion ◽  
Yield Value ◽  
Photodynamic Activity ◽  
Mcf 7

A novel Sn(IV) meso-tetraacenaphthylporphyrin (SnTAcP) has been synthesized and characterized. SnTAcP was complexed with methyl-[Formula: see text]-cyclodextrin (m[Formula: see text]-CD), a nanocarrier that enhances water solubility, and the complexes were evaluated as PDT agents using MCF-7 breast cancer cells. A relatively low singlet oxygen quantum yield value of 0.36 was obtained in DMF, and the lowest energy Q band lies at 608 nm on the edge of the therapeutic window. SnTAcP was found to be non-toxic in the dark and phototoxic towards MCF-7 breast cancer cells with a half-maximal inhibitory concentration (IC[Formula: see text] value of 11 ± 1.1 [Formula: see text]g · mL[Formula: see text] after 30 min of irradiation with a 625 nm Thorlabs LED that provides a dose of 432 J · cm[Formula: see text]. A higher IC[Formula: see text] value of 21 ± 1.1 [Formula: see text]g · mL-1 was obtained for the m[Formula: see text]-CD inclusion complex of SnTAcP.

Photodynamic activity of 2,6-dibrominated dimethylaminophenylbuta-1,3-dienylBODIPY dyes

2020 ◽  
Vol 25 (01) ◽  
pp. 47-55
Author(s):  
Gugu Kubheka ◽  
Balaji Babu ◽  
Earl Prinsloo ◽  
Nagao Kobayashi ◽  
John Mack ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Photodynamic Activity ◽  
Depth Study ◽  
Mcf 7

Mono- and disubstituted 2,6-dibromo-dimethylaminophenylbuta-1,3-dienylBODIPY dyes were successfully prepared, and their in vitro photodynamic activities against MCF-7 breast cancer cells were evaluated with a Thorlabs M660L4 660 nm LED (336 J · cm[Formula: see text]. The IC[Formula: see text] value of the monophenylbuta-1,3-dienylBODIPY was ca. 2.1 [Formula: see text]M, while that of the diphenylbuta-1,3-dienylBODIPY was > 50 [Formula: see text]M. Both dyes exhibited minimal dark toxicity. The results demonstrate that monosubstituted 2,6-dibromo-dimethylaminophenylbuta-1,3-dienylBODIPY dyes merit further in-depth study for use as photosensitizer dyes in photodynamic therapy.

Photodynamic activity of Sn(iv) tetrathien-2-ylchlorin against MCF-7 breast cancer cells

2021 ◽  
Vol 50 (6) ◽  
pp. 2177-2182
Author(s):  
Balaji Babu ◽  
John Mack ◽  
Tebello Nyokong
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Photodynamic Activity ◽  
Mcf 7

The utility of Sn(iv) tetraarylchlorins for use as photosensitizer dyes in photodynamic therapy is assessed.

In vitro survival of MCF-7 breast cancer cells following combined treatment with ionizing radiation and mitoxantrone-mediated photodynamic therapy

2013 ◽  
Vol 10 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Ameneh Sazgarnia ◽  
Ali Reza Montazerabadi ◽  
Mohammad Hossein Bahreyni-Toosi ◽  
Amirhossein Ahmadi ◽  
Amir Aledavood
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Combined Treatment ◽  
In Vitro Survival ◽  
Mcf 7

scholarly journals Multi-Modulation of Doxorubicin Resistance in Breast Cancer Cells by Poly(l-histidine)-Based Multifunctional Micelles

Pharmaceutics ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 385 ◽  
Author(s):  
Li Jia ◽  
Nan Jia ◽  
Yan Gao ◽  
Haiyang Hu ◽  
Xiuli Zhao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Multi Drug Resistance ◽  
Glycoprotein P ◽  
Passive Targeting ◽  
Poly Ethylene ◽  
Targeting Ability ◽  
Mcf 7

Even though the reversal of multi-drug resistance (MDR) by numerous nanoparticles has been extensively studied, limited success has been achieved. To overcome this barrier, we report a rationally-designed pH-sensitive micelle, in which doxorubicin (Dox) and resveratrol (Res) were co-loaded. The micelle was based on methoxy poly (ethylene glycol)-poly(d,l-lactide)-poly(l-histidine) (mPEG-PLA-PHis), which integrated passive targeting, endo-lysosomal escape and pH-responsive payloads release. At a physiological pH of 7.4 (slightly alkali), Dox and Res were incorporated into the micelles core using the thin-film hydration method (pH-endoSM/Dox/Res). After cellular uptake, the micelles exhibited an enhanced dissociation in response to the acidic endosomes, triggering the release of Res and Dox. Furthermore, Res was observed to synergistically improve the cytotoxicity of Dox by down-regulating the P-glycoprotein (P-gp) expression, decreasing the membrane potential of the mitochondrial and ATP level, as well as inducing cell apoptosis mediated by mitochondria. The pH-endoSM/Dox/Res showed a prominent ability to decrease the IC50 of Dox by a factor of 17.38 in cell cytotoxicity against the MCF-7/ADR cell line. In vivo distribution demonstrated the excellent tumor-targeting ability of the pH-endoSM/Dox/Res. All results indicated that pH-endoSM/Dox/Res held great potential for the treatment of Dox-resistance breast cancer cells.

Selective Photodynamic Effects on Breast Cancer Cells Provided by p123 Pluronic®- Based Nanoparticles Modulating Hypericin Delivery

2020 ◽  
Vol 20 (11) ◽  
pp. 1352-1367 ◽  
Author(s):  
Gabrielle Marconi Zago Ferreira Damke ◽  
Raquel Pantarotto Souza ◽  
Maiara Camotti Montanha ◽  
Edilson Damke ◽  
Renato Sonchini Gonçalves ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Fluorescence Microscopy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Unmet Need ◽  
Breast Cancers ◽  
Trypan Blue Exclusion ◽  
Mcf 7

Background: Breast cancer is the most relevant type of cancer and the second cause of cancer- related deaths among women in general. Currently, there is no effective treatment for breast cancer although advances in its initial diagnosis and treatment are available. Therefore, the value of novel anti-tumor therapeutic modalities remains an immediate unmet need in clinical practice. Following our previous work regarding the properties of the Pluronics with different photosensitizers (PS) for photodynamic therapy (PDT), in this study we aimed to evaluate the efficacy of supersaturated hypericin (HYP) encapsulated on Pluronic® P123 (HYP/P123) against breast cancer cells (MCF-7) and non-tumorigenic breast cells (MCF-10A). Methods: Cell internalization and subcellular distribution of HYP/P123 was confirmed by fluorescence microscopy. The phototoxicity and citototoxicity of HYP/P123 was assessed by trypan blue exclusion assay in the presence and absence of light. Long-term cytotoxicity was performed by clonogenic assay. Cell migration was determined by the wound-healing assay. Apoptosis and necrosis assays were performed by annexin VFITC/ propidium Iodide (PI) by fluorescence microscopy. Results: Our results showed that HYP/P123 micelles had high stability and high rates of binding to cells, which resulted in the selective internalization in MCF-7, indicating their potential to permeate the membrane of these cells. Moreover, HYP/P123 micelles accumulated in mitochondria and endoplasmic reticulum organelles, resulting in the photodynamic cell death by necrosis. Additionally, HYP/P123 micelles showed effective and selective time- and dose dependent phototoxic effects on MCF-7 cells but little damage to MCF-10A cells. HYP/P123 micelles inhibited the generation of cellular colonies, indicating a possible capability to prevent the recurrence of breast cancer. We also demonstrated that HYP/P123 micelles inhibit the migration of tumor cells, possibly by decreasing their ability to form metastases. Conclusion: Taken together, the results presented here indicate a potentially useful role of HYP/P123 micelles as a platform for HYP delivery to more specifically and effectively treat human breast cancers through photodynamic therapy, suggesting they are worthy for in vivo preclinical evaluations.

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