scholarly journals AIE-Driven Fluorescent Polysaccharide Polymersome as Enzyme-responsive FRET Nanoprobe to Study the Real-time Delivery Aspects in Live-Cells

2021 ◽  
Author(s):  
Nilesh Umakant Deshpande ◽  
Mishika Virmani ◽  
Manickam Jayakannan
Keyword(s):  
Energy Transfer ◽  
Confocal Microscopy ◽  
Real Time ◽  
Fluorescence Resonance Energy Transfer ◽  
Imaging Techniques ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cells ◽  
Intracellular Enzyme ◽  
Bio Imaging

We report aggregation induced emission (AIE) driven polysaccharide polymersome as fluorescence resonance energy transfer (FRET) nanoprobes to study their intracellular enzyme-responsive delivery by real-time live-cell confocal microscopy bio-imaging techniques. AIE...

scholarly journals Highly multiplexed imaging of biosensors in live cells

2020 ◽  
Author(s):  
Jr-Ming Yang ◽  
Wei-Yu Chi ◽  
Jessica Liang ◽  
Pablo Iglesias ◽  
Chuan-Hsiang Huang
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cells ◽  
Spectral Space ◽  
Fluorescent Biosensors ◽  
Multiplexed Imaging ◽  
Mixed Populations

AbstractFluorescent biosensors allow for real-time monitoring of biochemical activities in cells, but their multiplexing capacity is severely limited by the availability of spectral space. We overcome this problem by developing a set of barcoding proteins that are spectrally separable from commonly used FRET (fluorescence resonance energy transfer)-based and single-fluorophore biosensors. Mixed populations of barcoded cells expressing different biosensors can be concurrently imaged and computationally unmixed to achieve highly multiplexed tracking of biochemical activities in live cells.

scholarly journals Spectral wide-field microscopic fluorescence resonance energy transfer imaging in live cells

2015 ◽  
Vol 20 (8) ◽  
pp. 086011 ◽  
Author(s):  
Lili Zhang ◽  
Guiqi Qin ◽  
Liuying Chai ◽  
Jiang Zhang ◽  
Fangfang Yang ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cells ◽  
Wide Field ◽  
Fluorescence Resonance
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