Highly multiplexed imaging of biosensors in live cells
Keyword(s):
AbstractFluorescent biosensors allow for real-time monitoring of biochemical activities in cells, but their multiplexing capacity is severely limited by the availability of spectral space. We overcome this problem by developing a set of barcoding proteins that are spectrally separable from commonly used FRET (fluorescence resonance energy transfer)-based and single-fluorophore biosensors. Mixed populations of barcoded cells expressing different biosensors can be concurrently imaged and computationally unmixed to achieve highly multiplexed tracking of biochemical activities in live cells.
2015 ◽
Vol 20
(8)
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pp. 086011
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2012 ◽
Vol 87
(3)
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pp. 511-517
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2004 ◽
Vol 50
(6)
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pp. 1080-1082
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2007 ◽
Vol 92
(10)
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pp. 3564-3574
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2017 ◽
Vol 139
(28)
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pp. 9459-9462
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