scholarly journals Extraction and purification of ε-poly-l-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration

RSC Advances ◽  
2020 ◽  
Vol 10 (49) ◽  
pp. 29587-29593
Author(s):  
Xusheng Chen ◽  
Wenjiao Diao ◽  
Yu Ma ◽  
Zhonggui Mao
Keyword(s):  
Antimicrobial Agent ◽  
Ammonium Sulfate ◽  
Fermentation Broth ◽  
Phase System ◽  
Natural Antimicrobial ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Extraction And Purification

Natural antimicrobial agent ε-poly-l-lysine could be alternative prepared by ethanol/ammonium sulfate ATPS combined with ultrafiltration from the microbial feedstock.

Selection and optimization of an aqueous two-phase system for the recovery of 1,3-propandiol from fermentation broth

2010 ◽  
pp. NA-NA ◽  
Author(s):  
Özlem Aydoğan ◽  
Emine Bayraktar ◽  
Ülkü Mehmetoğlu ◽  
Thomas Kaeding ◽  
An-Ping Zeng
Keyword(s):  
Fermentation Broth ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Extraction of alkaline protease using an aqueous two-phase system from cell free Bacillus subtilis TISTR 25 fermentation broth

2005 ◽  
Vol 40 (11) ◽  
pp. 3514-3518 ◽  
Author(s):  
Wilaiwan Chouyyok ◽  
Nuntiya Wongmongkol ◽  
Napa Siwarungson ◽  
Seeroong Prichanont
Keyword(s):  
Bacillus Subtilis ◽  
Alkaline Protease ◽  
Fermentation Broth ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Extraction and purification of lipase from Enterococcus faecium MTCC5695 by PEG/phosphate aqueous-two phase system (ATPS) and its biochemical characterization

2016 ◽  
Vol 6 ◽  
pp. 19-27 ◽  
Author(s):  
Vrinda Ramakrishnan ◽  
Louella Concepta Goveas ◽  
Niranjan Suralikerimath ◽  
Chandrashekar Jampani ◽  
Prakash M. Halami ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Biochemical Characterization ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Extraction And Purification

The Extraction of Polyphenols from Tea Leaves Based on Mechanochemical Methodology and Aqueous Two-Phase System

2013 ◽  
Vol 834-836 ◽  
pp. 508-514
Author(s):  
Da Gang Li ◽  
Fu Fang Yuan ◽  
Ru Pan Chen ◽  
Yun Long Li ◽  
Kun Lu Li ◽  
...  
Keyword(s):  
Ammonium Sulfate ◽  
Room Temperature ◽  
Maximum Yield ◽  
Green Technology ◽  
Extraction System ◽  
Phase System ◽  
Two Phase ◽  
Material Particle ◽  
Aqueous Two Phase System ◽  
Optimum Parameters

In order to extract tea polyphenols (TPs) by water under room temperature, the mechanochemical methodology was used for leaching process, and then TPs was purified from leaching solution by ethanol and ammonium sulfate aqueous two-phase system. The optimum parameters of mechanochemical methodology leaching TPs were determined by response surface methodology (RSM), which optimum parameters were: Na2CO3content 25~27wt%, solid material particle size D9540~45μm, liquid/solid mass ratio55~60, leaching time 16~18min. The TPs leaching rate was 16.46%. When ammonium sulfate mass composition0.2, ethanol mass composition0.35 as the composite of the extraction system, TPs maximum extraction rate was 93.4%. The maximum yield of TPs was 15.25% on the average, with average purity was 96.54% ultimately. Under such technology, water is only solvent used in TPs leaching at room temperature, and aqueous two-phase system extraction system under mild conditions, avoid the use of toxic solvent, is a green technology for extracting TPs.

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