Tuning the strength and swelling of an injectable polysaccharide hydrogel and the subsequent release of a broad spectrum bacteriocin, nisin A

2020 ◽  
Vol 8 (18) ◽  
pp. 4029-4038 ◽  
Author(s):  
James Flynn ◽  
Edel Durack ◽  
Maurice N. Collins ◽  
Sarah P. Hudson
Keyword(s):  
Antimicrobial Peptide ◽  
Broad Spectrum ◽  
Crosslink Density ◽  
Glycol Chitosan ◽  
Gel Swelling ◽  
P Balance ◽  
Chitosan Content ◽  
Polysaccharide Hydrogel

Balance of glycol chitosan content and crosslink density modulates injectable gel swelling, strength and the release of an antimicrobial peptide.

scholarly journals Bee venom-derived antimicrobial peptide melectin has broad-spectrum potency, cell selectivity, and salt-resistant properties

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Su Jin Ko ◽  
Eunji Park ◽  
Alina Asandei ◽  
Jee-Young Choi ◽  
Seung-Chul Lee ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Broad Spectrum ◽  
Bee Venom ◽  
Cell Selectivity

Macrocyclic Hairpin Mimetics of the Cationic Antimicrobial Peptide Protegrin I: A New Family of Broad-Spectrum Antibiotics

ChemBioChem ◽  
2002 ◽  
Vol 3 (11) ◽  
pp. 1126-1133 ◽  
Author(s):  
Sasalu C. Shankaramma ◽  
Zafiria Athanassiou ◽  
Oliver Zerbe ◽  
Kerstin Moehle ◽  
Carole Mouton ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Broad Spectrum ◽  
Cationic Antimicrobial Peptide ◽  
New Family ◽  
Broad Spectrum Antibiotics

Broad-Spectrum Robust Direct Bactericidal Activity of Fish IFNφ1 Reveals an Antimicrobial Peptide–like Function for Type I IFNs in Vertebrates

2021 ◽  
Vol 206 (6) ◽  
pp. 1337-1347
Author(s):  
Xun Xiao ◽  
Wentao Zhu ◽  
Yanqi Zhang ◽  
Zhiwei Liao ◽  
Changsong Wu ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Bactericidal Activity ◽  
Broad Spectrum ◽  
Type I ◽  
Type I Ifns

scholarly journals The Design and Construction of K11: A Novelα-Helical Antimicrobial Peptide

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Huang Jin-Jiang ◽  
Lu Jin-Chun ◽  
Lu Min ◽  
Huang Qing-Shan ◽  
Li Guo-Dong
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Peptides ◽  
Antimicrobial Peptide ◽  
Broad Spectrum ◽  
Staphylococcus Epidermidis ◽  
Therapeutic Index ◽  
Klebsiella Pneumonia ◽  
Gram Negative Bacteria ◽  
Drug Resistant ◽  
Promising Candidate

Amphipathicα-helical antimicrobial peptides comprise a class of broad-spectrum agents that are used against pathogens. We designed a series of antimicrobial peptides, CP-P (KWKSFIKKLTSKFLHLAKKF) and its derivatives, and determined their minimum inhibitory concentrations (MICs) againstPseudomonas aeruginosa, their minimum hemolytic concentrations (MHCs) for human erythrocytes, and the Therapeutic Index (MHC/MIC ratio). We selected the derivative peptide K11, which had the highest therapeutic index (320) among the tested peptides, to determine the MICs against Gram-positive and Gram-negative bacteria and 22 clinical isolates includingAcinetobacter baumannii, methicillin-resistantStaphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis,andKlebsiella pneumonia. K11 exhibited low MICs (less than 10 μg/mL) and broad-spectrum antimicrobial activity, especially against clinically isolated drug-resistant pathogens. Therefore, these results indicate that K11 is a promising candidate antimicrobial peptide for further studies.

scholarly journals Evaluating the Bioactivity of a Novel Broad-Spectrum Antimicrobial Peptide Brevinin-1GHa from the Frog Skin Secretion of Hylarana guentheri and Its Analogues

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 413 ◽  
Author(s):  
Qi Chen ◽  
Peng Cheng ◽  
Chengbang Ma ◽  
Xinping Xi ◽  
Lei Wang ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptide ◽  
Broad Spectrum ◽  
Hemolytic Activity ◽  
High Performance ◽  
Disulfide Bridge ◽  
Reversed Phase ◽  
Central Position ◽  
Skin Secretion ◽  
Mass Spectral

Many antimicrobial peptides (AMPs) have been identified from the skin secretion of the frog Hylarana guentheri (H.guentheri), including Temporin, Brevinin-1, and Brevinin-2. In this study, an antimicrobial peptide named Brevinin-1GHa was identified for the first time by using ‘shotgun’ cloning. The primary structure was also confirmed through mass spectral analysis of the skin secretion purified by reversed-phase high-performance liquid chromatography (RP-HPLC). There was a Rana-box (CKISKKC) in the C-terminal of Brevinin-1GHa, which formed an intra-disulfide bridge. To detect the significance of Rana-box and reduce the hemolytic activity, we chemically synthesized Brevinin-1GHb (without Rana-box) and Brevinin-1GHc (Rana-box in central position). Brevinin-1GHa exhibited a strong and broad-spectrum antimicrobial activity against seven microorganisms, while Brevinin-1GHb only inhibited the growth of Staphylococcus aureus (S. aureus), which indicates Rana-box was necessary for the antimicrobial activity of Brevinin-1GHa. The results of Brevinin-1GHc suggested transferring Rana-box to the central position could reduce the hemolytic activity, but the antimicrobial activity also declined. Additionally, Brevinin-1GHa demonstrated the capability of permeating cell membrane and eliminating biofilm of S. aureus, Escherichia coli (E. coli), and Candida albicans (C. albicans). The discovery of this research may provide some novel insights into natural antimicrobial drug design.

scholarly journals Characterization of a Fish Antimicrobial Peptide: Gene Expression, Subcellular Localization, and Spectrum of Activity

2000 ◽  
Vol 44 (8) ◽  
pp. 2039-2045 ◽  
Author(s):  
Alexander M. Cole ◽  
Rabih O. Darouiche ◽  
Diana Legarda ◽  
Nancy Connell ◽  
Gill Diamond
Keyword(s):  
Antimicrobial Peptide ◽  
Host Defense ◽  
Broad Spectrum ◽  
Divalent Cations ◽  
Standard Test ◽  
Interferon Response ◽  
Upstream Region ◽  
Amino Terminal ◽  
High Concentrations ◽  
Peptide Gene

ABSTRACT Antimicrobial peptides are proposed to act as the first line of mucosal host defense by exerting broad-spectrum microbicidal activity against pathogenic microbes. Pleurocidin, a new 25-residue linear antimicrobial peptide, was recently isolated from the skin secretions of winter flounder (Pleuronectes americanus). The present study identifies the cDNA and gene encoding pleurocidin. The pleurocidin gene comprises four exons. Its upstream region demonstrates consensus binding sequences for transcription factors found in host defense genes in mammals, including sequences identical to the NF-IL6 and alpha and gamma interferon response elements. Pleurocidin is predicted to exist as a 68-residue prepropeptide that undergoes proteolytic cleavage of its amino-terminal signal and carboxy-terminal anionic propiece to form the active, mature peptide. Transmission electron microscopy localized pleurocidin to the mucin granules of skin and intestinal goblet cells. Significant synergy was shown to occur between pleurocidin and d-cycloserine targetingMycobacterium smegmatis. Pleurocidin was functionally active at physiologic concentrations of magnesium and calcium; however, high concentrations of these divalent cations ablated pleurocidin's activity against a standard test strain, Escherichia coliD31. Pleurocidin was tested against bacterial and fungal clinical isolates and showed broad-spectrum antimicrobial activity. Together, these data support the hypothesis that pleurocidin participates in innate mucosal immunity, and it may prove to be a beneficial therapeutic agent.

scholarly journals Short Alkylated Peptoid Mimics of Antimicrobial Lipopeptides

2010 ◽  
Vol 55 (1) ◽  
pp. 417-420 ◽  
Author(s):  
Nathaniel P. Chongsiriwatana ◽  
Tyler M. Miller ◽  
Modi Wetzler ◽  
Sergei Vakulenko ◽  
Amy J. Karlsson ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptide ◽  
Broad Spectrum ◽  
Antimicrobial Lipopeptides ◽  
The Creation ◽  
Peptoid Mimics ◽  
Antimicrobial Potency

ABSTRACTWe report the creation of alkylated poly-N-substituted glycine (peptoid) mimics of antimicrobial lipopeptides with alkyl tails ranging from 5 to 13 carbons. In several cases, alkylation significantly improved the selectivity of the peptoids with no loss in antimicrobial potency. Using this technique, we synthesized an antimicrobial peptoid only 5 monomers in length with selective, broad-spectrum antimicrobial activity as potent as previously reported dodecameric peptoids and the antimicrobial peptide pexiganan.

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