A novel DNA biosensor for ultrasensitive detection of DNA methyltransferase activity based on a high-density “hot spots” SERS substrate and rolling circle amplification strategy

The Analyst ◽  
2021 ◽  
Author(s):  
Shengjie Ge ◽  
Menglin Ran ◽  
Yu Mao ◽  
Yue Sun ◽  
Xinyu Zhou ◽  
...  
Keyword(s):  
Hot Spots ◽  
Dna Methyltransferase ◽  
Rolling Circle Amplification ◽  
High Density ◽  
Dna Biosensor ◽  
Ultrasensitive Detection ◽  
Sers Substrate ◽  
Methyltransferase Activity ◽  
Rolling Circle ◽  
Amplification Strategy

Herein, we proposed a novel biosensor based on the high-density “hot spots” Au@SiO2 array substrate and rolling circle amplification (RCA) strategy for ultrasensitive detection of the CpG methyltransferase (M.SssI) activity....

Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity

2016 ◽  
Vol 52 (12) ◽  
pp. 2517-2520 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Wei Jiang
Keyword(s):  
Dna Methyltransferase ◽  
Detection System ◽  
Rolling Circle Amplification ◽  
Molecular Probe ◽  
Sensitive Assay ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Rolling Circle ◽  
Amplification Strategy ◽  
Fluorescence Detection System

A novel fluorescence detection system is developed for DNA methyltransferase activity assay based on target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy.

A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

2014 ◽  
Vol 50 (13) ◽  
pp. 1576-1578 ◽  
Author(s):  
Liang Cui ◽  
Zhi Zhu ◽  
Ninghang Lin ◽  
Huimin Zhang ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle ◽  
Enzymatic Amplification ◽  
Amplification Method ◽  
Microrna Detection ◽  
Dual Amplification ◽  
Amplification Strategy ◽  
Excellent Selectivity ◽  
T7 Exonuclease

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA–CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

Sensitive detection of DNA methyltransferase activity based on rolling circle amplification technology

2014 ◽  
Vol 25 (7) ◽  
pp. 1047-1051 ◽  
Author(s):  
Pei Liu ◽  
Xiao-Hai Yang ◽  
Qing Wang ◽  
Jing Huang ◽  
Jian-Bo Liu ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Methyltransferase Activity ◽  
Rolling Circle

scholarly journals An integrated-molecular-beacon based multiple exponential strand displacement amplification strategy for ultrasensitive detection of DNA methyltransferase activity

2019 ◽  
Vol 10 (8) ◽  
pp. 2290-2297 ◽  
Author(s):  
Yun-Xi Cui ◽  
Xue-Nan Feng ◽  
Ya-Xin Wang ◽  
Hui-Yu Pan ◽  
Hua Pan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Molecular Beacon ◽  
Ultrasensitive Detection ◽  
Strand Displacement ◽  
Methyltransferase Activity ◽  
Strand Displacement Amplification ◽  
Multiple Signal ◽  
Amplification Strategy

An ultra-sensitive biosensor using only two DNA oligos to initiate multiple signal amplification cycles.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

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