Antihypertensive effect and underlying mechanism of tripeptide NCW on spontaneously hypertensive rats using metabolomics analysis

Antihypertensive Effect ◽

Underlying Mechanism ◽

Hypertensive Rats ◽

Ace Inhibitory Activity ◽

Ace Inhibitory ◽

Metabolomics Analysis

Tripeptide NCW identified in our previous study displayed strong ACE inhibitory activity, whether it has the antihypertensive effect in vivo remains unknown. Thus, in this paper, we aimed to investigate...

A novel angiotensin-I converting enzyme inhibitory peptide derived from the glutelin of vinegar soaked black soybean and its antihypertensive effect in spontaneously hypertensive rats

The Journal of Biochemistry ◽

10.1093/jb/mvz029 ◽

2019 ◽

Vol 166 (3) ◽

pp. 223-230 ◽

Cited By ~ 2

Author(s):

Yueyuan Zhang ◽

Yanling Zhang ◽

Peiyao Chen ◽

Fengjue Shu ◽

Kai Li ◽

...

Keyword(s):

Inhibitory Activity ◽

Converting Enzyme ◽

Hypertensive Rats ◽

Angiotensin I ◽

Ace Inhibitory Activity ◽

Black Soybean ◽

Angiotensin I Converting Enzyme ◽

Ace Inhibitory

AbstractVinegar soaked black soybean is a traditional Chinese food widely used for the treatment of hypertension. While its pharmacodynamic substance was not fully unveiled. It contained abundant glutelin, thus the purpose of this study was to obtain potent antihypertensive peptides from vinegar soaked black soybean. Black soybean was soaked with vinegar and then glutelin was first catalyzed by alcalase. Ultrafiltration, ion exchange chromatography and reversed-phase high performance liquid chromatography were sequentially applied to separate and purify the angiotensin-I converting enzyme (ACE) inhibitory peptides from glutelin hydrolysates. As a result, the fraction L1-4 with the highest ACE inhibitory activity (83.41%) at the final concentration of 0.01 mg/ml was obtained and five peptides were then identified. These peptides were further optimized by virtual screening combining with in silico proteolysis. Finally, a novel tetrapeptide Phe-Gly-Ser-Phe (FGSF) was obtained. FGSF exhibited high in vitro ACE inhibitory activity (IC50 = 117.11 μM) and in vivo hypotensive effect which maximally reduced systolic blood pressure of 21.95 mmHg at 20 mg/kg body weight in spontaneously hypertensive rats. Our study demonstrated that FGSF derived from vinegar soaked black soybean might be used as a promising ingredient for pharmaceuticals against hypertension and its related diseases.

Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats

Nutrients ◽

10.3390/nu12010271 ◽

2020 ◽

Vol 12 (1) ◽

pp. 271 ◽

Cited By ~ 3

Author(s):

Juan María Alcaide-Hidalgo ◽

Miguel Romero ◽

Juan Duarte ◽

Eduardo López-Huertas

Keyword(s):

Molecular Weight ◽

Inhibitory Activity ◽

Virgin Olive Oil ◽

Antihypertensive Activity ◽

Low Molecular Weight ◽

Hypertensive Rats ◽

Ace Inhibitory Activity ◽

Ace Inhibitory

The low molecular weight peptide composition of virgin olive oil (VOO) is mostly unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract from it. The peptides were separated by size-exclusion using fast protein liquid chromatography, and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences containing between 6 and 9 amino acids and molecular masses ranging 698–1017 Da. Those peptides were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration (IC50) <10 µm. The antihypertensive effects of the four most active synthesised ACE inhibitor peptides were studied in spontaneously hypertensive rats (SHR). Acute oral administration of synthetic peptides RDGGYCC and CCGNAVPQ showed antihypertensive activity in SHR. We conclude that unfiltered VOO naturally contains low molecular weight peptides with specific ACE inhibitory activity and antihypertensive effects in SHR.

Hypotensive and Angiotensin-Converting Enzyme Inhibitory Activities ofEisenia fetidaExtract in Spontaneously Hypertensive Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/349721 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Shumei Mao ◽

Chengde Li

Keyword(s):

Blood Pressure ◽

Inhibitory Activity ◽

Ang Ii ◽

Shr Rats ◽

Hypertensive Rats ◽

Ace Inhibitory Activity ◽

Ace Inhibitory

Objectives. This study aimed to investigate the antihypertensive effects of anEisenia fetidaextract (EFE) and its possible mechanisms in spontaneously hypertensive rats (SHR rats).Methods. Sixteen-week-old SHR rats and Wistar-Kyoto rats (WKY rats) were used in this study. Rats were, respectively, given EFE (EFE group), captopril (captopril group), or phosphate-buffered saline (PBS) (normal control group and SHR group) for 4 weeks. ACE inhibitory activity of EFEin vitrowas determined. The systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured using a Rat Tail-Cuff Blood Pressure System. Levels of angiotensin II (Ang II), aldosterone (Ald), and 6-keto-prostaglandin F1 alpha (6-keto-PGF1α) in plasma were determined by radioimmunoassay, and serum nitric oxide (NO) concentration was measured by Griess reagent systems.Results. EFE had marked ACE inhibitory activityin vitro(IC50= 2.5 mg/mL). After the 4-week drug management, SHR rats in EFE group and in captopril group had lower SBP and DBP, lower levels of Ang II and Ald, and higher levels of 6-keto-PGF1αand NO than the SHR rats in SHR group.Conclusion. These results indicate that EFE has hypotensive effects in SHR rats and its effects might be associated with its ACE inhibitory activity.