Densifying Lignocellulosic biomass with alkaline Chemicals (DLC) pretreatment unlocks highly fermentable sugars for bioethanol production from corn stover

2021 ◽  
Author(s):  
Xiangxue Chen ◽  
Xinchuan Yuan ◽  
Sitong Chen ◽  
Jianming Yu ◽  
Rui Zhai ◽  
...  

DLC pretreatment, which is simple and of low cost, not only facilitates biomass logistics but also provides a feedstock with high digestibility and high fermentability.

2020 ◽  
Vol 21 (2) ◽  
pp. 371 ◽  
Author(s):  
Yu Zhang ◽  
Jinshui Yang ◽  
Lijin Luo ◽  
Entao Wang ◽  
Ruonan Wang ◽  
...  

Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the commercial enzyme preparations, the T. harzianum EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting T. harzianum EM0925 enzymes have great potential for industrial applications.


2021 ◽  
Vol 10 (11) ◽  
pp. e149101118914
Author(s):  
Letícia Renata Bohn ◽  
Aline Perin Dresch ◽  
Matheus Cavali ◽  
Ana Carolina Giacomelli Vargas ◽  
Jaíne Flach Führ ◽  
...  

The demand for ethanol in Brazil is growing. However, although the country is one of the largest producers of this fuel, it is still necessary to diversify the production matrix. In that regard, studies with different raw materials are needed, mainly the use of low cost and high available wastes such as lignocellulosic residues from agriculture. Therefore, this study aimed to analyze the bioethanol production from corn stover. An alkaline pretreatment (CaO) was carried out, followed by enzymatic hydrolysis (Cellic Ctec2 and Cellic Htec2) to obtain fermentable sugars. The best experimental condition for the pretreatment and hydrolysis steps resulted in a solution with 0.31 gsugar∙gbiomass-1. Then, the fermentation was performed by the industrial strain of Saccharomyces cerevisiae (PE-2) and by the wild yeast strain Wickerhamomyces sp. (UFFS-CE-3.1.2). The yield obtained was 0.38 gethanol∙gdry biomass-1 was, demonstrating the potential of this process for bioethanol production.


ChemSusChem ◽  
2012 ◽  
Vol 6 (1) ◽  
pp. 110-122 ◽  
Author(s):  
Christos K. Nitsos ◽  
Konstantinos A. Matis ◽  
Kostas S. Triantafyllidis

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