Alkaline pretreatment and enzymatic hydrolysis of corn stover for bioethanol production

The demand for ethanol in Brazil is growing. However, although the country is one of the largest producers of this fuel, it is still necessary to diversify the production matrix. In that regard, studies with different raw materials are needed, mainly the use of low cost and high available wastes such as lignocellulosic residues from agriculture. Therefore, this study aimed to analyze the bioethanol production from corn stover. An alkaline pretreatment (CaO) was carried out, followed by enzymatic hydrolysis (Cellic Ctec2 and Cellic Htec2) to obtain fermentable sugars. The best experimental condition for the pretreatment and hydrolysis steps resulted in a solution with 0.31 gsugar∙gbiomass-1. Then, the fermentation was performed by the industrial strain of Saccharomyces cerevisiae (PE-2) and by the wild yeast strain Wickerhamomyces sp. (UFFS-CE-3.1.2). The yield obtained was 0.38 gethanol∙gdry biomass-1 was, demonstrating the potential of this process for bioethanol production.

Download Full-text

Investigation of Acid and Enzyme Wheat Straw Hydrolysis for Obtaining Polysaccharides

Oriental Journal Of Chemistry ◽

10.13005/ojc/350237 ◽

2019 ◽

Vol 35 (2) ◽

pp. 766-772

Author(s):

Zhaksylyk Baumanuly Makhatov ◽

Bakhytzhan Shilmirzaevich Kedelbayev ◽

Madina Dzhakashyeva ◽

Amina Daulbai ◽

Bibilgul Zaydullayevna Doltayeva ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Wheat Straw ◽

Plant Biomass ◽

Raw Materials ◽

Low Cost ◽

Economic Feasibility ◽

Aspergillus Awamori ◽

Processing Plant ◽

Pre Treatment ◽

Hydrolysis Of

The processes of acid and enzymatic hydrolysis of wheat straw in the presence of the strain Aspergillus awamori F-RKM 0719 has been studied. Enzymatic hydrolysis is the most promising method of processing plant biomass. When carrying out the enzymatic hydrolysis of cellulosic materials, the yield of sugars reaches less than 20% of the theoretically possible yield. Overcoming the physico-chemical barriers that hamper the availability of cellulose for enzymes is an important issue, the solution of which is directly related to the search for low-cost pre-treatment methods for raw materials. The effectiveness of this process determines the yield of the target product in the process of enzymatic hydrolysis of cellulose and the economic feasibility of the entire technology as a whole.

Download Full-text

Enzymatic hydrolysis of starch- and cellulose-containing raw materials to fermentable sugars in bioethanol production

FOOD RESOURCES ◽

10.31073/foodresources2019-13-12 ◽

2019 ◽

Vol 7 (13) ◽

pp. 121-130

Author(s):

Serhii Oliynichuk ◽

◽

Kateryna Danilova ◽

Keyword(s):

Enzymatic Hydrolysis ◽

Raw Materials ◽

Fermentable Sugars ◽

Bioethanol Production ◽

Hydrolysis Of

Download Full-text

Techno-Economic Analysis of Bioethanol Production from Lignocellulosic Biomass in China: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover

Energies ◽

10.3390/en8054096 ◽

2015 ◽

Vol 8 (5) ◽

pp. 4096-4117 ◽

Cited By ~ 40

Author(s):

Lili Zhao ◽

Xiliang Zhang ◽

Jie Xu ◽

Xunmin Ou ◽

Shiyan Chang ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Economic Analysis ◽

Corn Stover ◽

Lignocellulosic Biomass ◽

Dilute Acid Pretreatment ◽

Bioethanol Production ◽

Dilute Acid ◽

Acid Pretreatment ◽

Hydrolysis Of

Download Full-text

Effect of removing hemicellulose and lignin synchronously under mild conditions on enzymatic hydrolysis of corn stover

Fuel Processing Technology ◽

10.1016/j.fuproc.2020.106407 ◽

2020 ◽

Vol 204 ◽

pp. 106407 ◽

Cited By ~ 2

Author(s):

Shengxin An ◽

Wenzhi Li ◽

Fengyang Xue ◽

Xu Li ◽

Ying Xia ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Corn Stover ◽

Mild Conditions ◽

Hydrolysis Of

Download Full-text

Improvement of Enzymatic Saccharification of Cellulose-Containing Raw Materials Using Aspergillus niger

Processes ◽

10.3390/pr9081360 ◽

2021 ◽

Vol 9 (8) ◽

pp. 1360

Author(s):

Ekaterina Budenkova ◽

Stanislav Sukhikh ◽

Svetlana Ivanova ◽

Olga Babich ◽

Vyacheslav Dolganyuk ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Aspergillus Niger ◽

Filter Paper ◽

Raw Materials ◽

Enzymatic Saccharification ◽

Wood Chip ◽

Cellulase Activity ◽

Wood Chips ◽

Acid Pretreatment ◽

Hydrolysis Of

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.

Download Full-text

Effect of Mechanical Pretreatment for Enzymatic Hydrolysis of Woody Residues, Corn Stover and Alfalfa

Waste and Biomass Valorization ◽

10.1007/s12649-019-00856-x ◽

2019 ◽

Vol 11 (11) ◽

pp. 5847-5856 ◽

Cited By ~ 2

Author(s):

Ju Chen ◽

Kokou Adjallé ◽

Thanh Tung Lai ◽

Simon Barnabé ◽

Michel Perrier ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Corn Stover ◽

Mechanical Pretreatment ◽

Hydrolysis Of

Download Full-text

Effect on the enzymatic hydrolysis of lipids from dairy wastewater by replacing Gum Arabic emulsifier for sodium chloride

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132005000400017 ◽

2005 ◽

Vol 48 (spe) ◽

pp. 135-142 ◽

Cited By ~ 17

Author(s):

Adriano Aguiar Mendes ◽

Heizir Ferreira de Castro

Keyword(s):

Sodium Chloride ◽

Enzymatic Hydrolysis ◽

Suspended Solids ◽

Lower Cost ◽

Low Cost ◽

Gum Arabic ◽

Dairy Wastewater ◽

Promising Technique ◽

Animal Source ◽

Hydrolysis Of

The objective of this work was to evaluate the replacement of Gum Arabic for sodium chloride to reduce fat and organic contents in dairy wastewater using two low cost commercially available lipase preparations from animal source (Kin Master - LKM and Nuclear- LNU). The best performance was achieved when lipase Nuclear (LNU) was used as catalyst. In addition, this lipase preparation has also lower cost, which makes its use a quite promising technique for reduction of suspended solids as proteins and lipids contents found in wastewater generated by dairy industries.

Download Full-text