scholarly journals Probing Protein Aggregation at Buried Interfaces: Distinguishing Adsorbed Protein Monomer, Dimer, and Monomer-Dimer Mixture in Situ

2022 ◽  
Author(s):  
Tieyi Lu ◽  
Wen Guo ◽  
Datar M. Prathamesh ◽  
Yue Xin ◽  
E. Neil G. Marsh ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Protein Adsorption ◽  
Protein Drug ◽  
Protein Drugs ◽  
Biomedical Materials ◽  
Drug Adsorption ◽  
Separation Membranes ◽  
Buried Interfaces ◽  
Adsorbed Protein

Protein adsorption on surfaces greatly impacts many applications such as biomedical materials, anti-biofouling coatings, bio-separation membranes, biosensors, and antibody protein drugs etc. For example, protein drug adsorption on widely used...

scholarly journals Plasmon-enhanced two-channel in situ Kretschmann ellipsometry of protein adsorption, cellular adhesion and polyelectrolyte deposition on titania nanostructures

2016 ◽  
Vol 24 (5) ◽  
pp. 4812 ◽  
Author(s):  
Judit Nador ◽  
Benjamin Kalas ◽  
Andras Saftics ◽  
Emil Agocs ◽  
Peter Kozma ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Cellular Adhesion ◽  
Titania Nanostructures

A look into the interaction of metal oxide thin films with biological media: Albumin and Fibrinogen adsorption

2012 ◽  
Vol 1376 ◽  
Author(s):  
P. Silva-Bermudez ◽  
S. Muhl ◽  
M. Rivera ◽  
S. E. Rodil
Keyword(s):  
Surface Roughness ◽  
Surface Energy ◽  
Protein Adsorption ◽  
Photoelectron Spectroscopy ◽  
Ex Situ ◽  
Polar Component ◽  
Metal Oxide Thin Films ◽  
Adsorbed Protein ◽  
Major Chemical ◽  
Fibrinogen Adsorption

ABSTRACTIn the present work, the adsorption of albumin and fibrinogen on Ta, Nb, Ti and Zr oxidesthin films deposited on Si (100) wafers by magnetron sputtering was studied in order to get a better understanding of the correlation among the surface properties of these oxides and the protein adsorption phenomena on their surfaces. The surface energy, hydrophobicity, chemical composition, roughness and atomic order of the films were characterized. The films were immersedfor 45 minutes in single protein solutions; either albumin or fibrinogenand the adsorbed protein layer on the films was studied ex-situ in a dry ambient using bothX-ray photoelectron spectroscopy and atomic force microscopy.The adsorption of albumin and fibrinogen on the films modified the surface morphology and decreased the surface roughness for all the four different metal oxides. The XPS results confirmed the presence of the protein on the surface of the films and showed that the two proteins studied were adsorbed without undergoing a major chemical decomposition. A correlation between the surface roughness,the polar component of the surface energy of the films and the atomic percentage of nitrogen on the films after protein adsorption, an indirect signal of the amount of protein adsorbed, was found for albumin and fibrinogen adsorption on Ta, Nb and Ti oxides; the largest the roughness or the polar component the largest amount of adsorbed protein.

An in situ study of protein adsorption on combinatorial Cu–Al films using spectroscopic ellipsometry

2010 ◽  
Vol 81 (1) ◽  
pp. 58-66 ◽  
Author(s):  
M.A. McArthur ◽  
T.M. Byrne ◽  
R.J. Sanderson ◽  
G.P. Rockwell ◽  
L.B. Lohstreter ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Spectroscopic Ellipsometry ◽  
In Situ Study

scholarly journals Parallel chromatography and in situ scattering to interrogate competing protein aggregation pathways

2018 ◽  
Vol 27 (7) ◽  
pp. 1325-1333 ◽  
Author(s):  
Diana Gomes ◽  
Rebecca K. Kalman ◽  
Rebecca K. Pagels ◽  
Miguel A. Rodrigues ◽  
Christopher J. Roberts
Keyword(s):  
Protein Aggregation ◽  
In Situ Scattering

X-RAY DIFFRACTION FOR SURFACES AND BURIED INTERFACES

2000 ◽  
Vol 07 (04) ◽  
pp. 437-446 ◽  
Author(s):  
G. RENAUD
Keyword(s):  
Grazing Incidence ◽  
X Rays ◽  
X Ray Diffraction ◽  
X Ray ◽  
Grain Sizes ◽  
Buried Interfaces ◽  
Surfaces And Interfaces ◽  
Coherent Interfaces

The application of X-rays to the structural characterization of surfaces and interfaces, in situ and in UHV, is discussed on selected examples. Grazing incidence X-ray diffraction is not only a very powerful technique for quantitatively investigating the atomic structure of surfaces and interfaces, but is also very useful for providing information on the interfacial registry for coherent interfaces or on the strain deformation, island and grain sizes for incoherent epilayers.

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