Tripeptide-based macroporous hydrogel improves the osteogenic microenvironment of stem cells

Background: Three-dimensional (3D) culture has been reported to increase the therapeutic potential of mesenchymal stem cells (MSCs). The present study assessed the therapeutic efficacy of extracellular vesicles (EVs) from 3D cultures of human placental MSCs (hPMSCs) for acute kidney injury (AKI). Methods: The supernatants from monolayer culture (2D) and 3D culture of hPMSCs were ultra-centrifuged for EVs isolation. C57BL/6 male mice were submitted to 45 min bilateral ischemia of kidney, followed by renal intra-capsular administration of EVs within a 72 h reperfusion period. Histological, immunohistochemical, and ELISA analyses of kidney samples were performed to evaluate cell death and inflammation. Kidney function was evaluated by measuring serum creatinine and urea nitrogen. The miRNA expression profiles of EVs from 2D and 3D culture of hPMSCs were evaluated using miRNA microarray analysis. Results: The 3D culture of hPMSCs formed spheroids with different diameters depending on the cell density seeded. The hPMSCs produced significantly more EVs in 3D culture than in 2D culture. More importantly, injection of EVs from 3D culture of hPMSCs into mouse kidney with ischemia-reperfusion (I/R)-AKI was more beneficial in protecting from progression of I/R than those from 2D culture. The EVs from 3D culture of hPMSCs were more efficient against apoptosis and inflammation than those from 2D culture, which resulted in a reduction in tissue damage and amelioration of renal function. MicroRNA profiling analysis revealed that a set of microRNAs were significantly changed in EVs from 3D culture of hPMSCs, especially miR-93-5p. Conclusion: The EVs from 3D culture of hPMSCs have therapeutic potential for I/R-AKI.

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Effect of Purmorphamine on Osteogenic Differentiation of Human Mesenchymal Stem Cells in a Three-Dimensional Dynamic Culture System

Cellular and Molecular Bioengineering ◽

10.1007/s12195-014-0343-x ◽

2014 ◽

Vol 7 (4) ◽

pp. 575-584 ◽

Cited By ~ 5

Author(s):

Faezeh Faghihi ◽

Adam Papadimitropoulos ◽

Ivan Martin ◽

Mohamadreza Baghaban Eslaminejad

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Culture System ◽

Three Dimensional ◽

Human Mesenchymal Stem Cells ◽

Dynamic Culture

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Three-dimensional microenvironmental priming of human mesenchymal stem cells in hydrogels facilitates efficient and rapid retroviral gene transduction via accelerated cell cycle synchronization

NPG Asia Materials ◽

10.1038/s41427-019-0127-9 ◽

2019 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

Yein Lee ◽

Yoshie Arai ◽

Jinsung Ahn ◽

Deogil Kim ◽

Seunghee Oh ◽

...

Keyword(s):

Cell Cycle ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Three Dimensional ◽

Human Mesenchymal Stem Cells ◽

Gene Transduction ◽

Cell Cycle Synchronization

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Comparison of Regenerative Effects of Transplanting Three-Dimensional Longitudinal Scaffold Loaded-Human Mesenchymal Stem Cells and Human Neural Stem Cells on Spinal Cord Completely Transected Rats

ACS Biomaterials Science & Engineering ◽

10.1021/acsbiomaterials.9b01790 ◽

2020 ◽

Vol 6 (3) ◽

pp. 1671-1680 ◽

Cited By ~ 1

Author(s):

Yunlong Zou ◽

Yannan Zhao ◽

Zhifeng Xiao ◽

Bing Chen ◽

Dezun Ma ◽

...

Keyword(s):

Spinal Cord ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Neural Stem Cells ◽

Three Dimensional ◽

Human Mesenchymal Stem Cells ◽

Human Neural Stem Cells

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Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems

Stem Cells International ◽

10.1155/2019/7486279 ◽

2019 ◽

Vol 2019 ◽

pp. 1-16 ◽

Cited By ~ 18

Author(s):

Vitale Miceli ◽

Mariangela Pampalone ◽

Serena Vella ◽

Anna Paola Carreca ◽

Giandomenico Amico ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Three Dimensional ◽

3D Culture ◽

Culture Method ◽

Paracrine Factors ◽

Human Amnion ◽

Tissue Repair And Regeneration ◽

3D Culture System

The secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in the field of regenerative medicine. Indeed, the in vitro multipotency of these cells and the secretive capacity of both angiogenic and immunomodulatory factors suggest a role in tissue repair and regeneration. However, during culture, MSCs rapidly lose the expression of key transcription factors associated with multipotency and self-renewal, as well as the ability to produce functional paracrine factors. In our study, we show that a three-dimensional (3D) culture method is effective to induce MSC spheroid formation, to maintain the multipotency and to improve the paracrine activity of a specific population of human amnion-derived MSCs (hAMSCs). The regenerative potential of both 3D culture-derived conditioned medium (3D CM) and their exosomes (EXO) was assessed against 2D culture products. In particular, tubulogenesis assays revealed increased capillary maturation in the presence of 3D CM compared with both 2D CM and 2D EXO. Furthermore, 3D CM had a greater effect on inhibition of PBMC proliferation than both 2D CM and 2D EXO. To support this data, hAMSC spheroids kept in our 3D culture system remained viable and multipotent and secreted considerable amounts of both angiogenic and immunosuppressive factors, which were detected at lower levels in 2D cultures. This work reveals the placenta as an important source of MSCs that can be used for eventual clinical applications as cell-free therapies.

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Microstructured scaffold coated with hydroxyapatite/collagen nanocomposite multilayer for enhanced osteogenic induction of human mesenchymal stem cells

Journal of Materials Chemistry ◽

10.1039/c0jm01062f ◽

2010 ◽

Vol 20 (40) ◽

pp. 8927 ◽

Cited By ~ 28

Author(s):

Taek Gyoung Kim ◽

Suk-Hee Park ◽

Hyun Jung Chung ◽

Dong-Yol Yang ◽

Tae Gwan Park

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Human Mesenchymal Stem Cells ◽

Osteogenic Induction

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Mesenchymal stem cells from adipose tissue which have been differentiated into chondrocytes in three-dimensional culture express lubricin

Experimental Biology and Medicine ◽

10.1258/ebm.2011.011183 ◽

2011 ◽

Vol 236 (11) ◽

pp. 1333-1341 ◽

Cited By ~ 48

Author(s):

Giuseppe Musumeci ◽

Debora Lo Furno ◽

Carla Loreto ◽

Rosario Giuffrida ◽

Silvia Caggia ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Collagen Type ◽

Three Dimensional ◽

3D Culture ◽

Collagen Type I ◽

Type I ◽

Human Mscs ◽

Alternative Source

The present study focused on the isolation, cultivation and characterization of human mesenchymal stem cells (MSCs) from adipose tissue and on their differentiation into chondrocytes through the NH ChondroDiff medium. The main aim was to investigate some markers of biomechanical quality of cartilage, such as lubricin, and collagen type I and II. Little is known, in fact, about the ability of chondrocytes from human MSCs of adipose tissue to generate lubricin in three-dimensional (3D) culture. Lubricin, a 227.5-kDa mucinous glycoprotein, is known to play an important role in articular joint physiology, and the loss of accumulation of lubricin is thought to play a role in the pathology of osteoarthritis. Adipose tissue is an alternative source for the isolation of multipotent MSCs, which allows them to be obtained by a less invasive method and in larger quantities than from other sources. These cells can be isolated from cosmetic liposuctions in large numbers and easily grown under standard tissue culture conditions. 3D chondrocytes were assessed by histology (hematoxylin and eosin) and histochemistry (Alcian blue and Safranin-O/fast green staining). Collagen type I, II and lubricin expression was determined through immunohistochemistry and Western blot. The results showed that, compared with control cartilage and monolayer chondrocytes showing just collagen type I, chondrocytes from MSCs (CD44-, CD90- and CD105- positive; CD45-, CD14- and CD34-negative) of adipose tissue grown in nodules were able to express lubricin, and collagen type I and II, indicative of hyaline cartilage formation. Based on the function of lubricin in the joint cavity and disease and as a potential therapeutic agent, our results suggest that MSCs from adipose tissue are a promising cell source for tissue engineering of cartilage. Our results suggest that chondrocyte nodules producing lubricin could be a novel biotherapeutic approach for the treatment of cartilage abnormalities.

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