Stable Twisted Conformation Aza-BODIPY NIR-II Fluorescent Nanoparticles with Ultra-large Stokes Shift for Imaging-Guided Phototherapy

2022 ◽  
Author(s):  
Youliang Tian ◽  
Huiting Zhou ◽  
Quan Cheng ◽  
Huiping Dang ◽  
Hongyun Qian ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Stokes Shift ◽  
Great Promise ◽  
Deep Penetration ◽  
Fluorescent Nanoparticles ◽  
Spatiotemporal Resolution ◽  
Large Stokes Shift

Fluorescence imaging in the second near-infrared window (NIR-II, 1000–1700 nm) holds great promise for in vivo imaging and imaging-guided phototherapy with deep penetration and high spatiotemporal resolution. It is very...

A novel near-infrared fluorescent probe with a large Stokes shift for biothiol detection and application in in vitro and in vivo fluorescence imaging

2017 ◽  
Vol 5 (21) ◽  
pp. 3836-3841 ◽  
Author(s):  
Keyin Liu ◽  
Huiming Shang ◽  
Xiuqi Kong ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Stokes Shift ◽  
Large Stokes Shift ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging

A novel near-infrared fluorescent probe with a large Stokes shift was developed for biothiol detection and application in in vitro and in vivo fluorescence imaging.

The construction of a near-infrared fluorescent probe with dual advantages for imaging carbon monoxide in cells and in vivo

The Analyst ◽  
2021 ◽  
Vol 146 (1) ◽  
pp. 118-123
Author(s):  
Yang Tian ◽  
Wen-Li Jiang ◽  
Wen-Xin Wang ◽  
Jie Peng ◽  
Xue-Meng Li ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Stokes Shift ◽  
Emission Wavelength ◽  
Large Stokes Shift ◽  
In Cells

A novel near-infrared fluorescent probe (FDX-CO) is developed to detect CO with a large Stokes shift and long emission wavelength. The probe can monitor CO in cells by fluorescence imaging, and is successfully applied for visualizing CO in vivo.

scholarly journals Biocompatible and Biodegradable Magnesium Oxide Nanoparticles with In Vitro Photostable Near-Infrared Emission: Short-Term Fluorescent Markers

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1360 ◽  
Author(s):  
Asma Khalid ◽  
Romina Norello ◽  
Amanda N. Abraham ◽  
Jean-Philippe Tetienne ◽  
Timothy J. Karle ◽  
...  
Keyword(s):  
Magnesium Oxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Cultured Cells ◽  
Infrared Emission ◽  
Fluorescent Nanoparticles ◽  
Short Term ◽  
Fluorescent Markers

Imaging of biological matter by using fluorescent nanoparticles (NPs) is becoming a widespread method for in vitro imaging. However, currently there is no fluorescent NP that satisfies all necessary criteria for short-term in vivo imaging: biocompatibility, biodegradability, photostability, suitable wavelengths of absorbance and fluorescence that differ from tissue auto-fluorescence, and near infrared (NIR) emission. In this paper, we report on the photoluminescent properties of magnesium oxide (MgO) NPs that meet all these criteria. The optical defects, attributed to vanadium and chromium ion substitutional defects, emitting in the NIR, are observed at room temperature in NPs of commercial and in-house ball-milled MgO nanoparticles, respectively. As such, the NPs have been successfully integrated into cultured cells and photostable bright in vitro emission from NPs was recorded and analyzed. We expect that numerous biotechnological and medical applications will emerge as this nanomaterial satisfies all criteria for short-term in vivo imaging.

scholarly journals Orange Fluorescent Proteins: Filling the Spectral Gap

2014 ◽  
Vol 70 (a1) ◽  
pp. C1670-C1670
Author(s):  
Sergei Pletnev ◽  
Daria Shcherbakova ◽  
Oksana Subach ◽  
Vladimir Malashkevich ◽  
Steven Almo ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Spectral Properties ◽  
Molecular Mechanisms ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Stokes Shift ◽  
Emission Wavelength ◽  
Microscopy Imaging ◽  
Large Stokes Shift

Fluorescent proteins (FPs) have become valuable tools for molecular biology, biochemistry, medicine, and cancer research. Starting from parent green fluorescent protein (GFP), most challenging task of the FPs studies was the development of FPs with longer excitation/emission wavelength. This pursuit was motivated by advantages of so-called red-shifted FPs, namely, lower background of cellular autofluorescence in microscopy, lower light scattering and reduced tissue absorbance of longer wavelengths for in vivo imaging. In addition to FPs with regular spectral properties, there are proteins of other types available, including FPs with a large Stokes shift and photoconvertible FPs. These special kinds of FPs have become useful in super-resolution microscopy, imaging of enzyme activities, protein-protein interactions, photolabeling, and in vivo imaging. According to their emission wavelength, red-shifted FPs could be divided in the following groups: 520-540 nm yellow FPs (YFPs), 540-570 nm orange FPs (OFPs), 570-620 nm red FPs (RFPs), and > 620 nm far-RFPs. Red shift of the excitation/emission bands of these FPs is predominantly achieved by extension of the conjugated system of the chromophore and its protonation/deprotonation. The variety of spectral properties of FPs (excitation and emission wavelength, quantum yield, brightness, photo- and pH- stability, photoconversion, large Stokes shift, etc) results from the different chromophore structures and its interactions with surrounding amino acid residues. In this work we focus on structural studies and molecular mechanisms of FPs with orange emission.

scholarly journals Rational design of a NIR-II fluorescent nano-system with maximized fluorescence performance and applications

2021 ◽  
Author(s):  
Haoli Yu ◽  
Yuesong Wang ◽  
Yan Chen ◽  
Min Ji
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Nir Fluorescence

Purpose: Near-infrared (NIR) fluorescence imaging (FI) become a research hotspot in the field of in vivo imaging. Here, we intend to synthesize a NIR-II fluorescence nano-system with an excellent fluorescence...

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