scholarly journals Cell-surface mucosubstances from trypsin disaggregation of normal and virus-transformed lines of baby-hamster kidney cells (Short Communication)

1973 ◽  
Vol 134 (4) ◽  
pp. 1123-1126 ◽  
Author(s):  
S. Megan Minnikin ◽  
Adrian Allen
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Cell Lines ◽  
Short Communication ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Baby Hamster Kidney Cells

Cell disaggregation by trypsin solubilizes significantly less mucosubstance from the surface of polyoma-virus-transformed baby-hamster kidney cells than from the same non-transformed cell line. The mucosubstance, which consists of both acid mucopolysaccharides and mucoproteins, also differs qualitatively in the two cell lines.

Regulation of cell growth, c-myc mRNA, and 1,25-(OH)2 vitamin D3 receptor in C3H/10T1/2 mouse embryo fibroblasts by calcipotriol and 1,25-(OH)2 vitamin D3

1992 ◽  
Vol 126 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Torleif Trydal ◽  
Johan R. Lillehaug ◽  
Lage Aksnes ◽  
Dagfinn Aarskog
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Cell Lines ◽  
Vitamin D3 ◽  
Mouse Embryo ◽  
Transformed Cell ◽  
Mouse Embryo Fibroblasts ◽  
Transformed Cell Line ◽  
Embryo Fibroblasts ◽  
Transformed Cell Lines

Calcipotriol is a synthetic 1,25-(OH)2D3 analogue with high affinity for the 1,25-(OH)2D3 receptor, but with a lower affinity than 1,25-(OH)2D3 for vitamin D binding protein in serum. The inhibitory action of calcipotriol and 1,25-(OH)2D3 on proliferation of C3H/10T1/2 mouse embryo fibroblasts was examined in the non-transformed cell line CI 8 and in the two transformed, tumorigenic cell lines Cl 16 and TPA 482. Upon exposure to 10 nmol/l calcipotriol or 1,25-(OH)2D3, the proliferation of Cl 8 cell line was almost completely suppressed, whereas both hormones had no effect on the cell lines Cl 16 and TPA 482. Calcipotriol was at least as effective as 1,25-(OH)2D3 in inducing up-regulation of the 1,25-(OH)2D3 receptor. Displacement studies showed no difference between calcipotriol and 1,25-(OH)2D3 in the affinity for the receptor present in Cl 8 or Cl 16 cell extracts. Furthermore, the inhibition of cell growth in Cl 8 cells by calcipotriol was not accompanied by any consistent change in the steady-state expression of c-myc mRNA. In conclusion, calcipotriol had potent growth inhibitory effect on the non-transformed cell line similar to 1,25-(OH)2D3. In the transformed cell lines, calcipotriol did not inhibit proliferation despite potent up-regulation of the 1,25-(OH)2D3 receptor.

scholarly journals Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Katie L. Bailey ◽  
Sara B. Cartwright ◽  
Neesha S. Patel ◽  
Neeley Remmers ◽  
Audrey J. Lazenby ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Animal Model ◽  
Epithelial Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Large Animal Model ◽  
Large Animal ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Transformed Cell Lines

AbstractWe describe our initial studies in the development of an orthotopic, genetically defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.

scholarly journals Porcine pancreatic ductal epithelial cells transformed with KRAS G12D and SV40T are tumorigenic

2021 ◽  
Author(s):  
Katie L. Bailey ◽  
Sara B. Cartwright ◽  
Neesha S. Patel ◽  
Neeley Remmers ◽  
Audrey J. Lazenby ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Animal Model ◽  
Epithelial Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Large Animal Model ◽  
Large Animal ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Transformed Cell Lines

Abstract We describe our initial studies in the development of an orthotopic, genetically-defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.

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