Development and properties of fructose 1,6-bisphosphatase in the endosperm of castor-bean seedlings

1. The activity of fructose 1,6-bisphosphatase (EC 3.1.3.11) in the fatty endosperm of castor bean (Ricinus communis) increases 25-fold during germination and then declines. The developmental pattern follows that of catalase, a marker enzyme for gluconeogenesis in this tissue. 2. The enzyme at its peak of development was partially purified, and its properties were studied. It has an optimal activity at neutral pH (7.0-8.0). The apparent Km value for fructose 1,6-bisphosphate is 3.8 × 10-5 M. The activity is inhibited by AMP allosterically with an apparent Ki value of 2.2 × 10-4 M. The enzyme hydrolyses fructose 1,6-bisphosphate and not ribulose 1,5-bisphosphate or sedoehptulose 1,7-bisphosphate. 3. Treatment of the partially purified enzyme with acid leads to an 80% decrease in activity. The remaining activity is insensitive to AMP and has optimal activity at pH 6.7 and a high apparent Km value (2.5 × 10-4 M) for fructose 1.6-bisphosphate. Enzyme extracted from the tissue with water instead of buffer has a similar modification. The effect of acid explains the discrepancies between this report and previous ones on the properties of the enzyme in this tissue. 4. The storage tissues of various fatty seedlings all contain a ‘neutral’ fructose 1,6-bisphosphatase. The activities of the enzyme from some of the tissues are inhibited by AMP. 5. The properties of the enzyme in fatty seedlings and in green leaves are discussed in comparison with that in animal tissues.

Download Full-text

Partial purification and characterisation of cytosolic Fructose-1, 6-bisphosphatase from Drymaria cordata

Annals of Plant Sciences ◽

10.21746/aps.2018.7.4.9 ◽

2018 ◽

Vol 7 (4) ◽

pp. 2126

Author(s):

Niki Doma Sherpa ◽

Raksha Mukhia ◽

Dhani Raj Chhetri

Keyword(s):

Enzyme Activity ◽

Protein Concentration ◽

Biochemical Characterization ◽

Partial Purification ◽

Streptomycin Sulphate ◽

Fructose 1,6 Bisphosphatase ◽

Ethnomedicinal Plant ◽

Optimum Ph ◽

Km Value ◽

Fructose 1,6 Bisphosphate

Drymaria cordata is an important ethnomedicinal plant from which many important secondary metabolites have been reported. Partial purification of the enzyme, fructose 1,6-bisphosphatase was carried out following the methods of homogenization, streptomycin sulphate precipitation, ammonium sulphate cut and molecular sieve chromatography through Bio-Gel A-0.5m column. Biochemical characterization experiments were performed by standard methods with the enzyme preparation as purified from the column. Cytosolic fructose 1,6-bisphosphatase from the leaves of Drymaria cordata was purified to about 27-fold with 77% of recovery over homogenate fraction. The enzyme was highly specific to D-fructose-1,6-bisphosphate. With increase of protein concentration upto 300µg and incubation time upto120 minutes, the enzyme activity increased linearly. The metal ions Mg2+ or Mn2+ strongly stimulated the enzyme activity on the other hand Li+, Hg2+ and Zn2+ were potent inhibitors. The D. cordata enzyme showed temperature maxima at 40˚C while the optimum pH was at 8.0. The Km value of the enzyme for its substrate, Fructose 1,6-bisphosphate was 1.11µM proving its strong affinity.

Download Full-text

Gluconeogenic fructose-1,6-bisphosphatase from the mature sporocarps of common aquatic ferns: partial purification and basic characterization of this enzyme from Marsilea minuta (Polypodiopsida)

Ukrainian Botanical Journal ◽

10.15407/ukrbotj77.05.386 ◽

2020 ◽

Vol 77 (5) ◽

pp. 386-397

Author(s):

S.S. Ghosh ◽

◽

M. Das ◽

S. Basu ◽

J. Adhikari ◽

...

Keyword(s):

Partial Purification ◽

Light Illumination ◽

Azolla Pinnata ◽

Fructose 1,6 Bisphosphatase ◽

Salvinia Natans ◽

Km Value ◽

Gluconeogenic Enzyme ◽

Ph Range ◽

Fructose 1,6 Bisphosphate

The present communication reports substantial activity of gluconeogenic fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11) in three common heterosporous aquatic ferns (Marsilea minuta, Salvinia natans, and Azolla pinnata) and also describes a protocol for its partial purification from mature sporocarps of Marsilea minuta. The cytosolic FBPase, obtained from Marsilea minuta, Salvinia natans, and Azolla pinnata was recognized as gluconeogenic enzyme due to its drastic catabolic inactivation in presence of externally administered glucose and its insensitivity towards photosynthetic light illumination. Cytosolic gluconeogenic FBPase was partially purified from mature sporocarps of Marsilea minuta to about 22-fold over homogenate following low-speed centrifugation (11, 400 × g), 30–80% ammonium sulfate fractionation followed by subsequent chromatography using matrices like CM-Cellulose, Sephadex G-200, and Ultrogel AcA 34. The profile of partially purified FBPase in PAGE under non-denaturing condition was recorded. The enzyme activity increased linearly with respect to protein concentration to about 100 µg and with respect to time up to 75 minutes. Temperature optimum was found at 35 °C. The effect of substrate concentration and kinetic analyses for FBPase were carried out using D-fructose-1,6-bisphosphate (D-FBP, the substrate) in the range of 0.0 to 1.0 mM at an interval of 0.1 mM concentration. The Km value for D-FBP of FBPase was 0.06129 mM and Vmax was 4525 nmole Pi released (mg)-1 protein h-1 as determined by nonlinear regression kinetics using Prism 8 software (Graph Pad). The enzyme was functional in a constricted pH range of 7.0 to 8.0, giving maxima at pH 7.5. This cytosolic enzyme was significantly stimulated by Mg2+ and strongly inhibited by Hg2+, Cu2+ and Zn2+.

Download Full-text

On the origin of oil droplets in maturing castor bean seeds,Ricinus communis

Lipids ◽

10.1007/bf02531217 ◽

1971 ◽

Vol 6 (11) ◽

pp. 851-854 ◽

Cited By ~ 54

Author(s):

J. L. Harwood ◽

Ann Sodja ◽

P. K. Stumpf ◽

A. R. Spurr

Keyword(s):

Castor Bean ◽

Ricinus Communis ◽

Oil Droplets ◽

Origin Of Oil

Download Full-text

Ricinus communis L. (castor bean) as a potential candidate for revegetating industrial waste contaminated sites in peri-urban Greater Hyderabad: remarks on seed oil

Environmental Science and Pollution Research ◽

10.1007/s11356-017-9654-5 ◽

2017 ◽

Vol 24 (24) ◽

pp. 19955-19964 ◽

Cited By ~ 11

Author(s):

Ravi Kiran Boda ◽

Narasimha Vara Prasad Majeti ◽

Sateesh Suthari

Keyword(s):

Castor Bean ◽

Industrial Waste ◽

Seed Oil ◽

Ricinus Communis ◽

Contaminated Sites ◽

Potential Candidate ◽

Ricinus Communis L

Download Full-text

First report of association of Cucumber mosaic virus with blister and leaf distortion of castor bean (Ricinus communis) in India

Phytoparasitica ◽

10.1007/s12600-010-0096-6 ◽

2010 ◽

Vol 38 (3) ◽

pp. 283-289 ◽

Cited By ~ 7

Author(s):

Shri Krishna Raj ◽

Sunil Kumar Snehi ◽

Karmveer Kumar Gautam ◽

Mohammad Sajid Khan

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Castor Bean ◽

Ricinus Communis ◽

First Report ◽

Leaf Distortion

Download Full-text

Developing an agro-climatic zoning model to determine potential production areas for castor bean (Ricinus communis L.)

Industrial Crops and Products ◽

10.1016/j.indcrop.2012.02.044 ◽

2012 ◽

Vol 40 ◽

pp. 185-191 ◽

Cited By ~ 41

Author(s):

Silvia L. Falasca ◽

Ana C. Ulberich ◽

Eliana Ulberich

Keyword(s):

Castor Bean ◽

Ricinus Communis ◽

Potential Production ◽

Ricinus Communis L ◽

Production Areas ◽

Climatic Zoning

Download Full-text

Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean

Scientific Reports ◽

10.1038/s41598-021-85644-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Zaiqing Wang ◽

Anmin Yu ◽

Fei Li ◽

Wei Xu ◽

Bing Han ◽

...

Keyword(s):

Candidate Genes ◽

Castor Bean ◽

Woody Plants ◽

Molecular Mechanisms ◽

Ricinus Communis ◽

Bulked Segregant Analysis ◽

Oilseed Crop ◽

Yeast Transformation ◽

Dwarf Phenotype ◽

Genetic Breeding

AbstractPlant dwarfism is a desirable agronomic trait in non-timber trees, but little is known about the physiological and molecular mechanism underlying dwarfism in woody plants. Castor bean (Ricinus communis) is a typical woody oilseed crop. We performed cytological observations within xylem, phloem and cambia tissues, revealing that divergent cell growth in all tissues might play a role in the dwarf phenotype in cultivated castor bean. Based on bulked segregant analyses for a F2 population generated from the crossing of a tall and a dwarf accession, we identified two QTLs associated with plant height, covering 325 candidate genes. One of these, Rc5NG4-1 encoding a putative IAA transport protein localized in the tonoplast was functionally characterized. A non-synonymous SNP (altering the amino acid sequence from Y to C at position 218) differentiated the tall and dwarf plants and we confirmed, through heterologous yeast transformation, that the IAA uptake capacities of Rc5NG4-1Y and Rc5NG4-1C were significantly different. This study provides insights into the physiological and molecular mechanisms of dwarfing in woody non-timber economically important plants, with potential to aid in the genetic breeding of castor bean and other related crops.

Download Full-text

Life history of Neoseiulus californicus (McGregor, 1954) (Acari: Phytoseiidae) fed with castor bean (Ricinus communisL.) pollen in laboratory conditions

Brazilian Journal of Biology ◽

10.1590/bjb.2014.0079 ◽

2014 ◽

Vol 74 (3) ◽

pp. 691-697 ◽

Cited By ~ 9

Author(s):

PP Marafeli ◽

PR Reis ◽

EC. da Silveira ◽

GC Souza-Pimentel ◽

MA. de Toledo

Keyword(s):

Life History ◽

Castor Bean ◽

Ricinus Communis ◽

Intrinsic Rate ◽

Predatory Mite ◽

Neoseiulus Californicus ◽

Intrinsic Rate Of Increase ◽

Ricinus Communis L ◽

Rate Of Increase ◽

History Of

The predatory mite, Neoseiulus californicus(McGregor, 1954) (Acari: Phytoseiidae) is one of the principal natural enemies of tetranychid mites in several countries, promoting efficient control of those mites in several food and ornamental crops. Pest attacks such as that of the spider mite, Tetranychus urticaeKoch, 1836 (Acari: Tetranychidae), is one of the problems faced by farmers, especially in the greenhouse, due to the difficulty of its control with the use of chemicals because of the development of fast resistance making it hard to control it. The objective of this work was to study the life history of the predatory mite N. californicus as a contribution to its mass laboratory rearing, having castor bean plant [Ricinus communis L. (Euphorbiaceae)] pollen as food, for its subsequent use as a natural enemy of T. urticae on a cultivation of greenhouse rosebushes. The studies were carried out in the laboratory, at 25 ± 2°C of temperature, 70 ± 10% RH and a 14 hour photophase. The biological aspects and the fertility life table were appraised. Longevity of 32.9 days was verified for adult females and 40.4 days for males. The intrinsic rate of increase (rm) was 0.2 and the mean generation time (T) was 17.2 days. The population doubled every 4.1 days. The results obtained were similar to those in which the predatory mite N. californicus fed on T. urticae.

Download Full-text