scholarly journals The isolation and properties of a second glycoprotein (LGP-II) from the articular lubricating fraction from bovine synovial fluid

1979 ◽  
Vol 179 (3) ◽  
pp. 465-471 ◽  
Author(s):  
D A Swann ◽  
G Mintz
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Synovial Fluid ◽  
Bovine Serum ◽  
Gel Permeation Chromatography ◽  
Sedimentation Equilibrium ◽  
Major Constituent ◽  
Precipitin Line ◽  
Acetylneuraminic Acid ◽  
Gel Permeation

A high-molecular-weight glycoprotein (LGP-I) was shown [Swann, Sotman, Dixon & Brooks (1977) Biochem. J. 161, 473–485] to be the major constituent in the articular lubricating fraction from bovine synovial fluid. In addition to the LGP-I component, a second glycoprotein (LGP-II) was also present. After fractionation of bovine synovial fluid by sequential sedimentation in CsCl density gradients, the LGP-I and LGP-II components were separated by gel-permeation chromatography. The LGP-II component was then purified by chromatography on DEAE Bio-Gel A and Bio-Gel P-150. The molecular weight of the LGP-II component was 48,800 calculated from sedimentation-equilibrium measurements. Amino acids represented 53% (w/w) and carbohydrate constituents 36% (w/w) of the molecule. Glutamic acid and lysine (144 and 100 residues/1000 residues) were the major amino acids. Glucosamine, mannose, galactose and N-acetylneuraminic acid [representing 8.0, 6.6, 9.5 and 11.9% (w/w) respectively] were the only carbohydrate constituents detected. Immunodiffusion analysis showed that LGP-II component did not form a detectable precipitin line with antiserum to bovine serum. It appears likely, therefore, that this glycoprotein is synthesized by the joint tissues and is not derived from serum.

scholarly journals Chromatographic characterization of products isolated from chrome shavings

1999 ◽  
Vol 42 (3) ◽  
pp. 281-290
Author(s):  
Edivaldo E Garcia ◽  
Cláudia C.M Kimura ◽  
Ariovaldo C Martins ◽  
Gisele O Rocha ◽  
Jorge Nozaki
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Chemical Characterization ◽  
Gel Permeation Chromatography ◽  
Gel Permeation ◽  
Chromatographic Characterization ◽  
Free Aminoacids

Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Study on Structure and Tanning Properties of Copolymer of Resorcinol and 3,5-Dihydroxyl Benzoic Acid

2012 ◽  
Vol 487 ◽  
pp. 48-52
Author(s):  
Sheng Hua Lv ◽  
Gong Rui ◽  
Di Li
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Gel Permeation Chromatography ◽  
Radical Copolymerization ◽  
Mass Ratio ◽  
Monomer Composition ◽  
Chrome Tanning ◽  
Gel Permeation

The radical copolymerization of resorcinol (RSC) and 3,5-dihydroxyl benzoic (DHBA) was carried out in water by the initiator of horseradish (HRP)/H2O2. It was discussed that the effects of monomer composition on the properties of the copolymer. The best monomer mass ratio of RSC:DHBA was 60:40 and the shrink temperature can reached to 88.5oC. The tanning result indicated that the copolymer of RSC and DHPA has particularly excellent tanning properties and can be served as leather tannage substitute for chrome tanning materials. And also the mechanism of the HRP initiated copolymerization was proposed. The structure and molecular weight of the copolymer was characterized by Fourier Transform Infrared spectroscopy (FTIR), Nuclear Magnetic Resonance (NMR) and Gel Permeation Chromatography (GPC). The copolymer can be as tannage and retannage in making leather process. The results showed that it has excellent tanning properties and retanning effects.

scholarly journals Correlations between retention volume and molecular size parameters in gel permeation chromatography of small molecules

1975 ◽  
Vol 28 (1) ◽  
pp. 189 ◽  
Author(s):  
RA Shanks
Keyword(s):  
Molecular Weight ◽  
Small Molecules ◽  
Organic Molecules ◽  
Retention Volume ◽  
Molecular Size ◽  
Gel Permeation Chromatography ◽  
Molar Volumes ◽  
Small Organic Molecules ◽  
Molecular Dimension ◽  
Gel Permeation

Gel permeation columns of Bio Beads S-X8 have been used to provide separation of oligomers and other small organic molecules. Results show successful separations up to molecular weight c. 600. The retention times of compounds have been correlated with the largest molecular dimension of the molecules and also with molar volumes.

Alternative Tanning Agent for Leather Industry from a Sustainable Source

2021 ◽  
Vol 116 (3) ◽  
Author(s):  
Cigdem Kilicarislan Ozkan ◽  
Hasan Ozgunay
Keyword(s):  
Molecular Weight ◽  
Corn Starch ◽  
Periodate Oxidation ◽  
Gel Permeation Chromatography ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Leather Industry ◽  
Sodium Metaperiodate ◽  
Gel Permeation ◽  
Aldehyde Content

Dialdehyde starches with different aldehyde content from native corn starch were prepared by sodium periodate oxidation to be used as a tanning agent in leather making. For this purpose, native corn starch was oxidized with sodium metaperiodate in different molar ratios. After oxidation processes, the yields, solubility in water and aldehyde contents of the obtained dialdehyde starches were determined as well as structure characterizations by Proton Nuclear Magnetic Resonance Spectroscopy, Fourier Transform Infrared Spectroscopy and Gel Permeation Chromatography. Evaluating the gel permeation chromatography data, the dialdehyde starch samples which were thought to be in appropriate molecular weight/size to penetrate into skin fibers were selected to be used in the tanning process. Their tanning abilities were evaluated by investigating hydrothermal stabilities, filling and fiber isolation characteristics and physical properties determined by mechanical tests and organoleptically. From the evaluation of the results, it was revealed that sodium metaperiodate oxidized starches which have appropriate molecular weight and adequate aldehyde content has a remarkable tanning effect and can be utilized as a tanning agent with the advantages of not necessitating pickling process which means saving time and simplifying the production but more importantly offering an important advantage from an environmental point of view.

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