Tissue-culture Cell fractionation. Zonal centrifugation of Lettrée cells homogenized by glycerol-induced lysis: subfractionation of membranes in sucrose gradients
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1. Lettrée cells were grown intraperitoneally in MF-1 mice. 2. Cells that were loaded with glycerol were swollen in 0.1 M-sucrose and disrupted by Dounce homogenization. 3. Early-passage Lettrée cells were more easily disrupted than late-passage cells by this method, and the former produced larger fragments of plasma membrane. 4. The membranes were fractionated initially in sucrose gradients (on the basis of sedimentation rate) in a BXIV zonal rotor. 5. Fractions from this gradient were further resolved in isopycnic sucrose gradients. 6. Plasma-membrane and endoplasmic-reticulum fractions were recovered in good yield and high purity.
1976 ◽
Vol 4
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pp. 141-159
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1967 ◽
Vol 25
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pp. 132-133
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1978 ◽
Vol 189
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pp. 336-343
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1999 ◽
Vol 10
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pp. 1909-1922
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2008 ◽
Vol 24
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pp. 957-967
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1974 ◽
Vol 52
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pp. 345-352
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