[NiFe]-hydrogenase maturation in vitro: analysis of the roles of the HybG and HypD accessory proteins1
Keyword(s):
The present study demonstrates the in vitro reconstitution of active [NiFe]-hydrogenases from inactive intermediates. An anaerobically purified accessory protein complex comprising HybG–HypD and HypE completed maturation of apo-hydrogenases 1 and 2 in vitro yielding functional enzymes. The results suggest that a ternary complex carrying an intact Fe(CN)2CO moiety is required for reconstitution of enzyme activity.
1983 ◽
Vol 41
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pp. 432-433
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2007 ◽
Vol 2007
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pp. 214-215
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1999 ◽
Vol 81
(06)
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pp. 951-956
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2019 ◽
Vol 10
(1)
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1975 ◽
Vol 16
(5)
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pp. 1273-1281
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2003 ◽
Vol 31
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pp. 262-263
1984 ◽
Vol 259
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pp. 4496-4500
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