Effects of TGF-β1 and triiodothyronine on cartilage maturation: In vitro analysis using long-term high-density micromass cultures of chick embryonic limb mesenchymal cells

2006 ◽  
Vol 24 (11) ◽  
pp. 2095-2105 ◽  
Author(s):  
Maria A. Mello ◽  
Rocky S. Tuan
Keyword(s):  
Mesenchymal Cells ◽  
High Density ◽  
Maturation In Vitro ◽  
Embryonic Limb ◽  
Vitro Analysis ◽  
Tgf Β1 ◽  
In Vitro Analysis

Long-term in vitro analysis of limb cartilage development: Involvement of Wnt signaling

2004 ◽  
Vol 93 (3) ◽  
pp. 526-541 ◽  
Author(s):  
Kathleen M. Daumer ◽  
A. Cevik Tufan ◽  
Rocky S. Tuan
Keyword(s):  
Wnt Signaling ◽  
Cartilage Development ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Cariogenic and erosive potential of liquid oral pediatric medicines of long-term use for children: an in vitro analysis

2020 ◽  
Author(s):  
Ola B. Al-Batayneh ◽  
Ala'a B. Al-Bataina ◽  
Yousef S. Khader ◽  
Arwa I. Owais
Keyword(s):  
Dental Caries ◽  
Chronic Administration ◽  
Low Ph ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Vitro Analysis ◽  
High Sucrose ◽  
In Vitro Analysis

Abstract Background Liquid oral pediatric medicines (LOPM) contain sucrose and glucose and can have a low pH; their chronic administration may increase the risk for dental caries and erosion in children. The aim of this study was to determine sucrose and glucose concentrations, and pH of LOPMs of long-term use by children. Methods A cross sectional survey was conducted among 95/381 pharmacies to assess the most commonly prescribed LOPM by pharmacists in North Jordan, yielding 42 medicines which were analyzed for sucrose and glucose concentrations (mg/g) using HPLC-RID, and pH measurement using pH-meter CP-505. Data were analyzed using SPSS version 19.0. Differences were considered significant at P ≤ 0.05. Results The lowest and highest sucrose concentrations were for Ramlac® (0.9%) from the gastrointestinal medicines and Varolex® (79.5%) from the nutritional medicines, respectively. The lowest and highest glucose concentrations were for Adol® (0.0%) from the central nervous medicines and Pediavit-D® (81.1%) from the nutritional medicines, respectively. The pH ranged from 3.2 for Varolex® from the nutritional medicines to 10.0 for Zithromax® from the antibiotics medicines. Conclusions Some of the LOPMs studied had high sucrose or glucose concentrations (or both) and low pH which could increase their potential of causing dental caries and erosion when ingested frequently and chronically.

scholarly journals In vitro analysis of the feeding neural circuit in Aplysia after long‐term sensitization training

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Max Odem ◽  
Marcy Wainwright ◽  
Riccardo Mozzachiodi
Keyword(s):  
Neural Circuit ◽  
Vitro Analysis ◽  
In Vitro Analysis

The Significance of Surgery for Bulky N2 Small-Cell Lung Cancer: A Clinical and In Vitro Analysis of Long-Term Survivors

Surgery Today ◽  
2000 ◽  
Vol 30 (11) ◽  
pp. 978-986 ◽  
Author(s):  
Shunsuke Kobayashi ◽  
Shinichiro Okada ◽  
Toru Hasumi ◽  
Nobuyuki Sato ◽  
Shigefumi Fujimura
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Vitro Analysis ◽  
Bulky N2 ◽  
Long Term Survivors ◽  
In Vitro Analysis

scholarly journals The Effect of Different Cleaning Methods on Protein Deposition and Optical Characteristics of Orthokeratology Lenses

Polymers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 4318
Author(s):  
Chen-Ying Su ◽  
Lung-Kun Yeh ◽  
Yi-Fei Tsao ◽  
Wen-Pin Lin ◽  
Chiun-Ho Hou ◽  
...  
Keyword(s):  
Optical Characteristics ◽  
Short Term ◽  
Protein Deposition ◽  
Tear Proteins ◽  
Cleaning Methods ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Myopia Control

Orthokeratology lenses are commonly used for myopia control, especially in children. Tear lipids and proteins are immediately adsorbed when the lens is put on the cornea, and protein deposition may cause discomfort or infection. Therefore, we established an in vitro protein deposition analysis by mimicking the current cleaning methods for orthokeratology lens wearers for both short-term and long-term period. The results showed that the amounts of tear proteins accumulated daily and achieved a balance after 14 days when the lens was rubbed to clean or not. Protein deposition also affected the optical characteristics of the lens regardless of cleaning methods. Our results provided an in vitro analysis for protein deposition on the lens, and they may provide a potential effective method for developing care solutions or methods that can more effectively remove tear components from orthokeratology lenses.

In vitro analysis of the epstein-barr virus: Host balance in long-term renal allograft recipients

1985 ◽  
Vol 35 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Q. Y. Yao ◽  
A. B. Rickinson ◽  
J. S. H. Gaston ◽  
M. A. Epstein
Keyword(s):  
Epstein Barr Virus ◽  
Renal Allograft ◽  
Barr Virus ◽  
Epstein Barr ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals [NiFe]-hydrogenase maturation in vitro: analysis of the roles of the HybG and HypD accessory proteins1

2014 ◽  
Vol 464 (2) ◽  
pp. 169-177 ◽  
Author(s):  
Basem Soboh ◽  
Ute Lindenstrauss ◽  
Claudia Granich ◽  
Mahwish Javed ◽  
Martin Herzberg ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Protein Complex ◽  
Ternary Complex ◽  
Accessory Protein ◽  
Hydrogenase Maturation ◽  
Maturation In Vitro ◽  
In Vitro Reconstitution ◽  
Vitro Analysis ◽  
In Vitro Analysis

The present study demonstrates the in vitro reconstitution of active [NiFe]-hydrogenases from inactive intermediates. An anaerobically purified accessory protein complex comprising HybG–HypD and HypE completed maturation of apo-hydrogenases 1 and 2 in vitro yielding functional enzymes. The results suggest that a ternary complex carrying an intact Fe(CN)2CO moiety is required for reconstitution of enzyme activity.

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

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