Endocytosis of liposomes bound to cell surface proteins measured by flow cytofluorometry
Keyword(s):
A new technique for the quantification of cellular receptor-mediated endocytosis has been developed based on the analysis by flow cytometry of ligand-bearing liposomes containing the fluorochrome carboxyfluorescein. Carboxyfluorescein encapsulated at high concentrations in protein A-bearing liposomes is self-quenched. Binding and internalization of such liposomes by cells via antibodies directed towards membrane surface determinants results in the release of the liposome-encapsulated carboxyfluorescein into the cytoplasm causing an increase in cell-associated fluorescence. This increase can be quantified on a flow cytofluorometer.
1998 ◽
Vol 180
(12)
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pp. 3181-3186
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1979 ◽
Vol 80
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pp. 642-650
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1987 ◽
Vol 127
(3)
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pp. 373-376
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1995 ◽
Vol 53
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pp. 786-787
1979 ◽
Vol 254
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pp. 3935-3946
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