Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38)
Keyword(s):
Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion proteins. The identification, molecular cloning and sequencing are described of a type 1 membrane protein (TGN38) which is located specifically in the trans-Golgi network.
1992 ◽
Vol 116
(1)
◽
pp. 85-94
◽
1988 ◽
Vol 167
(6)
◽
pp. 1975-1980
◽
Keyword(s):
1993 ◽
Vol 13
(12)
◽
pp. 7625-7635
◽
Keyword(s):
1992 ◽
Vol 116
(6)
◽
pp. 1303-1317
◽
Keyword(s):
1999 ◽
Vol 10
(10)
◽
pp. 3435-3447
◽
Keyword(s):