scholarly journals Isolation of pig mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene promoter: characterization of a peroxisome proliferator-responsive element

1999 ◽  
Vol 337 (2) ◽  
pp. 329-335 ◽  
Author(s):  
José A. ORTIZ ◽  
Judith MALLOLAS ◽  
Carine NICOT ◽  
Josep BOFARULL ◽  
Joan C. RODRÍGUEZ ◽  
...  
Keyword(s):  
Promoter Region ◽  
Gene Promoter ◽  
Direct Repeat ◽  
Peroxisome Proliferator ◽  
Transcription Start ◽  
Promoter Characterization ◽  
Isolation And Characterization ◽  
Responsive Element

Low expression of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase gene during development correlates with an unusually low hepatic ketogenic capacity and lack of hyperketonaemia in piglets. Here we report the isolation and characterization of the 5´ end of the pig mitochondrial HMG-CoA synthase gene. The 581 bp region proximal to the transcription start site permits transcription of a reporter gene, confirming the function of the promoter. The pig mitochondrial HMG-CoA synthase promoter is trans-activated by the peroxisomal proliferator-activated receptor (PPAR), and a functional response element for PPAR (PPRE) has been localized in the promoter region. Pig PPRE is constituted by an imperfect direct repeat (DR-1) and a downstream sequence, both of which are needed to confer PPAR-sensitivity to a thymidine kinase promoter and to form complexes with PPAR·retinoid X receptor heterodimers. A role of PPAR trans-activation in starvation-associated induction of gene expression is suggested.

scholarly journals Isolation of pig mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene promoter: characterization of a peroxisome proliferator-responsive element

1999 ◽  
Vol 337 (2) ◽  
pp. 329 ◽  
Author(s):  
José A. ORTIZ ◽  
Judith MALLOLAS ◽  
Carine NICOT ◽  
Josep BOFARULL ◽  
Joan C. RODRÍGUEZ ◽  
...  
Keyword(s):  
Gene Promoter ◽  
Peroxisome Proliferator ◽  
Promoter Characterization ◽  
Responsive Element

scholarly journals Isolation and Characterization of thenikR Gene Encoding a Nickel-Responsive Regulator inEscherichia coli

1999 ◽  
Vol 181 (2) ◽  
pp. 670-674 ◽  
Author(s):  
Karinne De Pina ◽  
Valerie Desjardin ◽  
Marie-Andree Mandrand-Berthelot ◽  
Gerard Giordano ◽  
Long-Fei Wu
Keyword(s):  
Promoter Region ◽  
Constitutive Expression ◽  
Inescherichia Coli ◽  
Start Site ◽  
High Concentration ◽  
Transcription Start ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Specific Transport System

ABSTRACT Expression of the nickel-specific transport system encoded by theEscherichia coli nikABCDE operon is repressed by a high concentration of nickel. By using random transposon Tn10 insertion, we isolated mutants in which expression of the nik operon became constitutive with respect to nickel. We have identified the corresponding nikR gene which encodes a nickel-responsive regulator. Expression of nikRwas partially controlled by Fnr through transcription from thenikA promoter region. In addition, a specific transcription start site for the constitutive expression of nikR was found 51 bp upstream of the nikR gene.

scholarly journals Characterization of the human pleiotrophin gene. Promoter region and chromosomal localization.

1992 ◽  
Vol 267 (36) ◽  
pp. 26011-26016 ◽  
Author(s):  
Y.S. Li ◽  
R.M. Hoffman ◽  
M.M. Le Beau ◽  
R Espinosa ◽  
N.A. Jenkins ◽  
...  
Keyword(s):  
Promoter Region ◽  
Chromosomal Localization ◽  
Gene Promoter ◽  
Gene Promoter Region

Isolation and characterization of SRF accessory proteins

1993 ◽  
Vol 340 (1293) ◽  
pp. 325-332 ◽  
Keyword(s):  
Ternary Complex ◽  
Map Kinases ◽  
Serum Response Factor ◽  
Regulatory Element ◽  
Isolation And Characterization ◽  
Activation Of Transcription ◽  
Serum Response ◽  
Dna Binding Properties

Many genes which are regulated by growth factors contain a common regulatory element, the serum response element (SRE). Activation of transcription by the SRE involves a ternary complex formed between a ubiquitous factor, serum response factor (SRF), and a second protein, p62/TCF. We used a yeast genetic screen to isolate cDNAs encoding a protein, SAP-1, with the DNA binding properties of p62/TCF. The SAP-1 sequence contains three regions of homology to the previously uncharacterized Elk-1 protein, which also acts as an SRF accessory protein. Only two of these regions are required for cooperative interactions with SRF in the ternary complex. The third contains several conserved sites for the MAP kinases, whose activity is regulated in response to growth factor stimulation. We discuss the potential role of these proteins in regulation of the c-fos SRE.

scholarly journals Isolation and Characterization of a 1.7-kb Transposable Element from a Mutator Line of Maize

Genetics ◽  
1987 ◽  
Vol 117 (2) ◽  
pp. 297-307
Author(s):  
Loverine P Taylor ◽  
Virginia Walbot
Keyword(s):  
Direct Repeat ◽  
Repeat Sequence ◽  
Gene Probe ◽  
Gene Transcript ◽  
Chimeric Transcript ◽  
Purple Pigment ◽  
Isolation And Characterization ◽  
Internal Sequence ◽  
Normal Gene

ABSTRACT We have cloned and sequenced a 1.7-kb Mu element from a Mutator line of maize and compared its structure to Mu1, a 1.4-kb element. With the exception of a 385-bp block of DNA present in the 1.7-kb element, these transposable elements are structurally similar, sharing terminally inverted and internal direct repeated sequences. Derivation of 1.4-kb elements from the 1.7-kb class via deletion of internal sequence is suggested by the finding that a portion of the extra DNA in Mu1.7 is part of a truncated direct repeat sequence in the 1.4-kb element. An abundant poly(A)+ RNA homologous to a portion of this extra DNA is present in several tissues of both Mutator and non-Mutator lines. Analysis of transcripts from an unstable mutant bronze1 (bz) allele containing a Mu1.7 element inserted in an exon of the gene detects three species of poly(A)+ RNA that hybridize to a Bz1 (Bronze) gene probe: the largest contains the entire Mu1.7 element in the Bz1 gene transcript; another appears to be a spliced, chimeric transcript; the smallest is normal size Bz1 mRNA. The latter is most likely encoded by the normal-size alleles detected by Southern analysis of tissue expressing purple pigment, suggesting that normal gene function is restored by excision of the Mu1.7 element.

scholarly journals Association of polymorphisms in the promoter region of turkey prolactin with egg performance

Genetika ◽  
2014 ◽  
Vol 46 (2) ◽  
pp. 591-599 ◽  
Author(s):  
Mehrangiz Fathi ◽  
Ghorban Elyasi-Zarringhobaie
Keyword(s):  
Promoter Region ◽  
Egg Production ◽  
Gene Promoter ◽  
Reproductive Traits ◽  
Egg Mass ◽  
Breeding Center ◽  
Egg Performance ◽  
East Azerbaijan ◽  
Prolactin Promoter

The induction and regulation of broodiness is of the most important role of prolactin in avian species. In this study, the association between prolactin promoter region alleles and reproductive traits in Fars native turkey was investigated. These traits consisted of mean egg weight (MEW), number of egg (EN) and egg mass, during the first laying period. In total, 115 laying turkeys, randomly selected from the flock of the Breeding Center for Fars Native turkey, and DNA was purificated from blood samples, 231 bp of prolactin promoter region was amplified and Genotype of Samples was determinate by PCR-SSCP technique were genotyped. Two alleles D and I were identified. Based on the results obtained, the frequency of D and I alleles were 0.67 and 0.33, respectively. Frequencies of DD, II and ID genotypes were 0.385, 0.044 and 0.571, respectively. The association analysis between the polymorphism PRL gene promoter region and egg performance was carried out. Significant relationship was found between genotypes with egg production (P<0.01). Individuals with II genotype produced higher egg production than DD and ID genotype. The results of current study showed that using information of genes related to egg production could be used to improve the performance of native turkey of East Azerbaijan province.

The role of Golgi bodies in polysaccharide sulphation in Fucuszygotes

1978 ◽  
Vol 32 (1) ◽  
pp. 337-356
Author(s):  
M.E. Callow ◽  
S.J. Coughlan ◽  
L.V. Evans
Keyword(s):  
Cell Wall ◽  
Alginic Acid ◽  
Soluble Fraction ◽  
Amorphous Layer ◽  
Acceptor Molecule ◽  
Golgi Bodies ◽  
Isolation And Characterization ◽  
Fucus Serratus

The cell wall of 24-h zygotes of Fucus serratus is composed of 3 layers—an inner fibrillar layer (sulphated fucan), an outer fibrillar layer (alginic aicd/cellulose) and an exterior amorphous layer (sulphated fucan, alginic acid). The 2 layers containing sulphated fucan are preferentially thickened at the rhizoid pole. Light- and electron-microscope autoradiographic pulse-chase experiments on 22-h zygotes using 35SO2-(4) show the Golgi bodies to be the sites of fucan sulphation. The isolation and characterization of isolated Golgi-rich fractions from 22-h zygotes shows that the first detectable labelled macromolecule is associated with these fractions 2 min after addition of 35SO2-(4). The sulphate acceptor molecule has been partially characterized. 35S-APS and 35S-paps are detectable in the soluble fraction 0.5 min after addition of 35SO2-(4). The results are discussed in relation to other published work on the differentiation of Fucus embryos and on polysaccharide sulphation.

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