scholarly journals A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

2013 ◽  
Vol 33 (6) ◽  
Author(s):  
Weng-Tat Chan ◽  
Chandra S. Verma ◽  
David P. Lane ◽  
Samuel Ken-En Gan
Keyword(s):  
Escherichia Coli ◽  
Transformation Efficiency ◽  
Research Paper ◽  
Factors Affecting ◽  
Competent Cells ◽  
Common Laboratory

This research paper aims to investigate the factors affecting the production of competent cells to produce high levels of transformation efficiency in common laboratory strains.

Transformation of Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620 by CaCl2 method

2010 ◽  
Vol 56 (1) ◽  
pp. 74-76 ◽  
Author(s):  
Urmi Patel ◽  
Sarika Sinha
Keyword(s):  
Escherichia Coli ◽  
Optical Density ◽  
Sinorhizobium Meliloti ◽  
Transformation Efficiency ◽  
Binary Vector ◽  
Transformation Method ◽  
Mesorhizobium Ciceri ◽  
Competent Cells

The CaCl2 method, commonly used for transformation of Escherichia coli, was modified and used to develop a simpler and easier transformation method for Rhizobia sp. Two species of Rhizobia, Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620, were transformed with the 13.2 kb binary vector pGA482. At an optical density of 0.4, the transformation efficiencies in Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620 were 104 and 103, respectively. Competent cells of Sinorhizobium meliloti MTCC 100 were prepared at different growth intervals and transformed by the same vector. A maximum transformation efficiency of 104 was achieved at an optical density of 0.5.

Consumer Online Buying Decisions: A Critical Appraisal of Literature

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Amit Kishore Sinha ◽  
Gyanendra B. S. Johri ◽  
Shanti Rai
Keyword(s):  
Research Paper ◽  
Business Environment ◽  
Buying Behavior ◽  
Related Factors ◽  
Factors Affecting ◽  
Goods And Services ◽  
Online Stores ◽  
Online Buying ◽  
Indian Cities ◽  
Buying Decisions

Since last two decades buying of goods and services from online stores using Internet started off. But players of this industry could reach to the general public residing in second and third category Indian cities in recent past only. Now companies are eagerly interested in understanding the factors affecting Indian consumers so that their needs and wants can be understood and served profitably. This research paper is an attempt to critically evaluate those factors which affect consumer buying behavior in Indian Internet based business environment. For the purpose of coverage of topic researcher has classified the literature under three categories which are Literature related to vendor related factors, Literature related to consumer related factors and Literature related to other factors. Vendor related factors include those factors which are primarily controlled by the companies that are engaged in selling their goods and services on internet along with their intermediaries through which such sales take place. Consumer related factors have been bifurcated under two heading that are consumer demographic factors and consumer psychographic factors. Besides this there are several other factors which may affect consumer’s buying decisions and they are classified as other factors. This research paper also tries to identify the gaps (if any) in the available literature of the factors affecting consumer online buying decisions.

One-Step Preparation of Competent E. coli: Transformation and Storage of Bacterial Cells in the Same Solution

2021 ◽  
Vol 2021 (11) ◽  
pp. pdb.prot101212 ◽  
Author(s):  
Michael R. Green ◽  
Joseph Sambrook
Keyword(s):  
Escherichia Coli ◽  
Convenient Method ◽  
Plasmid Dna ◽  
Transformation Efficiency ◽  
Bacterial Cells ◽  
E Coli ◽  
One Step ◽  
And Storage

This protocol describes a convenient method for the preparation, use, and storage of competent Escherichia coli. The reported transformation efficiency of this method is ∼5 × 107 transformants/µg of plasmid DNA.

scholarly journals Factors affecting transformation efficiency of BCG with aMycobacterium-Escherichia colishuttle vector pYUB18 by electroporation

1998 ◽  
Vol 39 (2) ◽  
pp. 141 ◽  
Author(s):  
Sang Nae Cho ◽  
Jin Hee Hwang ◽  
Sun Park ◽  
Yunsup Chong ◽  
Sung Kyu Kim ◽  
...  
Keyword(s):  
Transformation Efficiency ◽  
Factors Affecting

scholarly journals Reclassification of Shigella species as later heterotypic synonyms of Escherichia coli in the Genome Taxonomy Database

2021 ◽  
Author(s):  
Donovan H Parks ◽  
Maria Chuvochina ◽  
Peter R Reeves ◽  
Scott A Beatson ◽  
Philip Hugenholtz
Keyword(s):  
Escherichia Coli ◽  
Type Strain ◽  
Shigella Flexneri ◽  
Laboratory Strain ◽  
Single Species ◽  
Species Definition ◽  
E Coli ◽  
Shigella Species ◽  
K 12 ◽  
Common Laboratory

Members of the genus Shigella have high genomic similarity to Escherichia coli and are often considered to be atypical members of this species. In an attempt to retain Shigella species as recognizable entities, they were reclassified as Escherichia species in the Genome Taxonomy Database (GTDB) using an operational average nucleotide identity (ANI)-based approach nucleated around type strains. This resulted in nearly 80% of E. coli genomes being reclassified to new species including the common laboratory strain E. coli K-12 (to 'E. flexneri') because it is more closely related to the type strain of Shigella flexneri than it is to the type strain of E. coli. Here we resolve this conundrum by treating Shigella species as later heterotypic synonyms of E. coli, present evidence supporting this reclassification, and show that assigning E. coli/Shigella strains to a single species is congruent with the GTDB-adopted genomic species definition.

scholarly journals Effect of Gold Nanoparticles Size Capped with Surfactant on the Transformation of Plasmid into Escherichia coli Bacteria

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Amr D ◽  
◽  
Attia N ◽  
Seufi A ◽  
Galal A ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Heat Shock ◽  
Transformation Efficiency ◽  
Transformation Process ◽  
Bacterial Cells ◽  
Bacterial Transformation ◽  
Foreign Genes ◽  
Escherichia Coli Bacteria ◽  
Convenient Technique

Bacterial transformation has great importance in molecular biology, as it is used for introduction of foreign genes into bacterial cells either chemical or physical ways. Using calcium chloride to prepare competent cells and heat shock is the most widely used method for bacterial transformation. This method is an efficient and convenient technique but it has in some extent low transformation efficiency. Here we report the use of nanoparticles that significantly improve the transformation efficiency up to 10 times higher than the standard heat shock method by the assistance of (˜ 15, 25 nm) SDS capped gold nanoparticles in the transformation process that leads to the formation of temporary nano-channels across the bacterial cell wall to deliver plasmids into cells. Transformation of bacteria with plasmid was examined using Β-galactosidase assay.

Improved long-term cryostorage of Escherichia coli competent cells using trehalose

2004 ◽  
Vol 26 (20) ◽  
pp. 1593-1594 ◽  
Author(s):  
Taeho Ahn ◽  
Seong Su Kang ◽  
Chul-Ho Yun
Keyword(s):  
Escherichia Coli ◽  
Competent Cells

scholarly journals Analysing Enabling Factors Affecting the On-site Productivity in Indian Construction Industry

2018 ◽  
Vol 49 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Saurav Dixit
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Reliability Analysis ◽  
Construction Industry ◽  
Pearson Correlation ◽  
Linear Regression Analysis ◽  
Research Paper ◽  
Site Productivity ◽  
Factors Affecting ◽  
Construction Productivity

The growth in construction productivity is low and do not continue for a long span of time. The purpose of the present research paper is to analyse the factors affecting on-site construction productivity in Indian construction industry. The tools used for the analysis are reliability analysis to check the consistency of the data, Pearson correlation analysis to mathematically validate the factor analysis, and linear regression analysis to propose a framework of factors affecting construction productivity. The findings of the reliability analysis concludes that the all the factors having a value of more than 0.67, which is considered to be good for the study, and the findings of the study conclude that the most significant 3 attributes grouped in linear regression analysis change in scope (R2), revision in drawings (R15), and response to change orders (R3) having maximum impact over the construction productivity. The research paper attempts to provide an insight and better understanding of the factors affecting on-site construction productivity in India and the ways and means to control and improve construction productivity of construction projects."This paper is the revised version of the paper that has been published in the proceedings of the Creative Construction Conference 2018: Dixit, S., Mandal, S. N., Thanikal, J. V, & Saurabh, K. (2018). Critical Analysis of Factors Affecting the on-site Productivity in Indian Construction Industry, (July), 38–45. https://doi.org/10.3311/CCC2018-006"

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