scholarly journals Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Dong Yang ◽  
Jun-hua Xu ◽  
Ren-jie Shi
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Anal Fistula ◽  
Healing Time ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Protein Levels ◽  
Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

scholarly journals Transient Epigenetic Reprogramming Human Skin Fibroblasts Differentiates into Cells with Multipotentiality In Vitro and In Vivo

CYTOLOGIA ◽  
2012 ◽  
Vol 77 (2) ◽  
pp. 251-259
Author(s):  
Xiang-Qing Zhu ◽  
Guang-Ping Ruan ◽  
Qing-Hua Chen ◽  
Rong-Qing Pang ◽  
Xue-Ming Cai ◽  
...  
Keyword(s):  
Human Skin ◽  
Epigenetic Reprogramming ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

In Vitro Cytotoxicity Tests on Cultured Human Skin Fibroblasts to Predict the Irritation Potential of Surfactants

1991 ◽  
Vol 19 (3) ◽  
pp. 324-336
Author(s):  
Marleen Cornells ◽  
Charlotte Dupont ◽  
Jacques Wepierre
Keyword(s):  
Human Skin ◽  
Calf Serum ◽  
In Vitro Cytotoxicity ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Experimental Conditions ◽  
System A ◽  
Brij 35

A comparative study of the cytotoxicity of 17 surfactants was performed in vitro on cultured human skin fibroblasts to predict their irritation potential under different experimental conditions: test media, presence of proteins, various times of exposure (2–72 hours), and evaluation methods. For cytotoxicity, the tetrazolium MTT assay after exposure for 2 hours in Hank's medium (HBSS) seemed to be more sensitive than protein and LDH leakage tests. Cytotoxicities in HBSS and in minimum Eagle's medium (MEM) were very similar. Addition of 10% fetal calf serum (FCS) to MEM decreased the cytotoxicity of surfactants; however, their order of cytotoxicity was generally the same in MEM with or without FCS. Cytotoxicity increased with incubation time, but the overall ranking remained identical. Non-ionic polyoxyethylene 20 ethers (Brij 35, 58, 78 and 99) surfactants, although considered to be non-irritant in vivo, revealed a high cytotoxic effect in our cell culture system. A good correlation with the results of in vivo Draize rabbit eye irritancy was found only when they were excluded.

scholarly journals WOUND HEALING PROPERTIES OF SWIETENIA MAHAGONI SEED EXTRACTED USING SCCO2: AN IN VITRO STUDY

2018 ◽  
Vol 81 (1) ◽  
Author(s):  
Hartati Hartati ◽  
Liza Md Salleh ◽  
Irma Suryani Idris ◽  
Andi Asmawati Azis
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
In Vitro Study ◽  
Seed Extract ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Scratch Assay ◽  
Healing Agent ◽  
Swietenia Mahagoni

Swietenia mahagoni seed has been traditionally used as a wound healing agent. The aim of this study was to investigate the in vitro healing properties of Swietenia mahagoni seed extract. A supercritical carbon dioxide (SCCO2) extract of Swietenia mahagoni seeds was tested regarding its ability to stimulate the growth of human skin fibroblasts (HSF 1184) in vitro using the colorimetric methylthiazol tetrazolium (MTT) assay and scratch assay in a selected range of extract concentrations. The results show that the Swietenia mahagoni seed extract at a concentration of 0.01 mg/mL was able to stimulate the growth of human skin fibroblasts. The scratch assay showed that all of the extracts led to a significantly higher percentage of wound closure compared to the control. The wound healing capabilities of Swietenia mahagoni seed extract may be due to its fibroblast stimulation activity, which may promote repair of the wounded dermis. Thus, it may serve as a good herbal component to incorporate into products designed to promote wound healing.

Previous chronic exogenous glucocorticoid administration in vivo does not affect functional characteristics and cellular lifespan of human skin fibroblasts in vitro

2011 ◽  
Vol 20 (6) ◽  
pp. 529-531 ◽  
Author(s):  
Harris Pratsinis ◽  
Anastasia Dimozi ◽  
Konstantinos Pilichos ◽  
Stylianos Tsagarakis ◽  
Andreas M Yiacoumettis ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Functional Characteristics ◽  
Glucocorticoid Administration

scholarly journals The Beneficial Effect of Rosmarinic Acid on Benzophenone-3-Induced Alterations in Human Skin Fibroblasts

2021 ◽  
Vol 22 (21) ◽  
pp. 11451
Author(s):  
Anna Galicka ◽  
Joanna Sutkowska-Skolimowska
Keyword(s):  
Human Skin ◽  
Rosmarinic Acid ◽  
Type I Collagen ◽  
Skin Fibroblasts ◽  
Percutaneous Penetration ◽  
Systemic Absorption ◽  
Type I ◽  
Human Skin Fibroblasts

Benzophenone-3 (BP-3) is one of the most widely used chemical sunscreens. The results of many in vitro and in vivo tests confirm its high percutaneous penetration and systemic absorption, which question the safety of its wide use. The aim of our research was to assess the effect of this compound on components of the skin extracellular matrix, and to investigate whether rosmarinic acid (RA) could reduce BP-3-induced changes in human skin fibroblasts. BP-3 used at concentrations of 0.1–100 µM caused a number of unfavorable changes in the level of type I collagen, decorin, sulfated glycosaminoglycans, hyaluronic acid, elastin, and expression or activity of matrix metalloproteinases (MMP-1, MMP-2), elastase and hyaluronidase. Moreover, the intracellular retention of collagen was accompanied by changes in the expression of proteins modifying and controlling the synthesis and secretion of this protein. Most importantly, RA at a concentration of 100 µM significantly reduced or completely abolished the adverse effects of BP-3. Based on these findings, it can be concluded that this polyphenol may provide effective protection against BP-3-induced disturbances in skin cells, which may have important clinical implications.

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