scholarly journals Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages

2019 ◽  
Vol 39 (2) ◽  
Author(s):  
Xihang Chen ◽  
Yunfan He ◽  
Anqi Xu ◽  
Zilong Deng ◽  
Jingwei Feng ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Epithelial Cell ◽  
Tissue Regeneration ◽  
Volume Expansion ◽  
Early Stage ◽  
Surgical Reconstruction ◽  
Control Group ◽  
Cell Clusters ◽  
Glandular Epithelial Cell ◽  
External Volume

AbstractBackground: There is a clinical need for the use of engineered adipose tissue in place of surgical reconstruction. We previously found that the external volume expansion (EVE) device increased special cell clusters in well-vascularized connective stroma during adipose regeneration. However, the origin of these cell clusters and their role in adipose tissue regeneration remain unknown. Aim: In the present study, we evaluated EVE in the construction of expanded prefabricated adipose tissue (EPAT) in a rat model. Methods: Rats were randomized into an EVE suction group and a control group, with 24 rats in each group. The structure and origin of the special cell clusters were determined by hematoxylin and eosin staining, and immunohistochemistry; their role in adipose tissue regeneration was investigated by immunohistochemistry and Western blot analyses. Results: Special cell clusters began to increase at week 1 with a peak at week 4, and then receded from weeks 8 to 12. Clusters were identified as glandular epithelial cells as determined by their gland-like structure and expression of specific markers. The cell clusters induced significant infiltration of macrophage antigen-2 (Mac-2) positive macrophages by secreting monocyte chemoattractant protein-1 (MCP-1) at the early stage of suction. Subsequently, these infiltrated macrophages expressed massive vascular endothelial growth factor (VEGF) to promoted angiogenesis. Conclusion: EVE generated glandular epithelial cell clusters, which recruited macrophages to promote angiogenesis and subsequent adipose tissue regeneration. These findings shed light on the mechanisms underlying the effects of EVE devices on adipose tissue regeneration.

The Combination of Tissue Dissection and External Volume Expansion Generates Large Volumes of Adipose Tissue

2017 ◽  
Vol 139 (4) ◽  
pp. 888e-899e ◽  
Author(s):  
Yunfan He ◽  
Ziqing Dong ◽  
Gan Xie ◽  
Tao Zhou ◽  
Feng Lu
Keyword(s):  
Adipose Tissue ◽  
Volume Expansion ◽  
Tissue Dissection ◽  
External Volume

Delayed Postconditioning with External Volume Expansion Improves Survival of Adipose Tissue Grafts in a Murine Model

2019 ◽  
Vol 143 (1) ◽  
pp. 99e-110e ◽  
Author(s):  
Shuyi Wei ◽  
Wenyue Liu ◽  
Buket Gundogan ◽  
Andrea V. Moscoso ◽  
Dennis P. Orgill ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Murine Model ◽  
Volume Expansion ◽  
External Volume ◽  
Tissue Grafts

Construction of Expanded Prefabricated Adipose Tissue Using an External Volume Expansion Device

2017 ◽  
Vol 139 (5) ◽  
pp. 1129-1137 ◽  
Author(s):  
Yi Yuan ◽  
Shuifa Yang ◽  
Yangyan Yi ◽  
Jianhua Gao ◽  
Feng Lu
Keyword(s):  
Adipose Tissue ◽  
Volume Expansion ◽  
External Volume

scholarly journals Effect of external volume expansion on the survival of fat grafts

2016 ◽  
Vol 49 (02) ◽  
pp. 151-158 ◽  
Author(s):  
Raghuveer Reddy ◽  
Subramania Iyer ◽  
Mohit Sharma ◽  
Sundeep Vijayaraghavan ◽  
P. Kishore ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Volume Expansion ◽  
Small Sample Size ◽  
Small Sample ◽  
Positive Pressure ◽  
Time Intervals ◽  
Variable Time ◽  
External Volume ◽  
Tissue Grafts ◽  
Volume Retention

ABSTRACT Introduction: External volume expansion (EVE) is one method, which has been utilised for increasing the survival of adipose tissue grafts. EVE releases positive pressure from the graft and also induces intense levels of edema that decreases diffusion of metabolites essential for graft survival initially. The ideal timing of external volume expansion in relation to the injection of the fat to facilitate survival is not yet clear. Aims and Objectives: This study was undertaken to evaluate and compare the efficacy of external volume expansion applied at variable time points in relation to the injection of the fat. Materials and Methods: Athymic mouse was the animal model and human lipo-aspirate mixed with PRP was used as graft. An indigenous dome shaped silicone device was fabricated to deliver a negative pressure of -30 mm of Hg. The EVE was applied at variable time intervals. At the end of 4 weeks visual, histological and radiological features of the injected fat were compared. The adipose tissue was stained with human vimentin to ascertain the origin of the retained fat. Results: All the grafts, which had EVE, had significantly better volume retention and vascularity. The groups which underwent a delayed EVE or prior expansion followed by concomitant graft injection and expansion showed the most optimal vascularity and graft retention. Conclusions: A delayed EVE or prior expansion followed by concomitant graft injection and expansion may be the most ideal combinations to optimize graft take. However, on account of the relatively small sample size, there was a limitation in drawing statistically significant conclusions for certain variables.

Biological and Phytochemical Screening of Fumaria indica extract on Chemically Induced Hepatocellular Carcinoma with Reference to Biochemical Parameters

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Irfan Aziz ◽  
Birendra Shrivastava ◽  
Chandana Venkateswara Rao2 ◽  
Sadath Ali
Keyword(s):  
Free Radicals ◽  
Liver Disease ◽  
Liver Cancer ◽  
Early Stage ◽  
Food Additives ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Industrial Chemicals ◽  
Toxic Industrial Chemicals

Liver disease or liver cancer is the sixth most common cancer and the third leading cause of cancer mortality in the world. Hepatitis viral infection, food additives, alcohol, fungal toxins (aflatoxins), toxic industrial chemicals, air and water pollutants are the major risk factors of liver cancer. Moreover, due to high tolerance of liver, HCC is seldom detected at an early stage and once detected treatment faces a poor prognosis in most cases.Fumaria indica possesses hepatoprotective activity as evidenced by the significant and dose dependent restoring the activities of entire liver cancer marker enzymes, diminution in tumor incidence, decrease in lipid peroxidation (LPO) and increase in the level of antioxidant enzymes (GSH, CAT, SOD, GPx and GST) through scavenging of free radicals, or by enhancing the activity of antioxidant, which then detoxify free radicals. These factors protect cells from ROS damage in NDEA and CCl4-induced hepatocarcinogenesis. Histopathological observations of liver tissues too correlated with the biochemical observations. Thus, present investigation suggested that the Fumaria indica would exert a chemoprotective effect by reversing the oxidant-antioxidant imbalance during hepatocarcinogenesis induced by NDEA and CCl4. Besides Fumaria indicais very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various liver injury and biochemical tumour markers enzymes. The hepatoprotective activity of a Fumaria indicaof 50 % ethanolic extract was studied using rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt. Fumaria indica extract dose dependently and significantly the increase in serum hepatic enzyme levels after NDEAand CCl4 treatment compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the Fumaria indicaextract against carbon tetrachlorideand N-nitrosodiethylamine induced hepatotoxicity in rats. In addition to this, studies on molecular aspect of hepatoprotective therapy will give mechanistic information in hepatoprotective therapy and also critical balance should be there between the animal model and clinical research. The hepatoprotective properties of Fumaria indicashould provide useful information in the possible application in hepatic liver disease.

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