Biological and Phytochemical Screening of Fumaria indica extract on Chemically Induced Hepatocellular Carcinoma with Reference to Biochemical Parameters

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Irfan Aziz ◽  
Birendra Shrivastava ◽  
Chandana Venkateswara Rao2 ◽  
Sadath Ali
Keyword(s):  
Free Radicals ◽  
Liver Disease ◽  
Liver Cancer ◽  
Early Stage ◽  
Food Additives ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Industrial Chemicals ◽  
Toxic Industrial Chemicals

Liver disease or liver cancer is the sixth most common cancer and the third leading cause of cancer mortality in the world. Hepatitis viral infection, food additives, alcohol, fungal toxins (aflatoxins), toxic industrial chemicals, air and water pollutants are the major risk factors of liver cancer. Moreover, due to high tolerance of liver, HCC is seldom detected at an early stage and once detected treatment faces a poor prognosis in most cases.Fumaria indica possesses hepatoprotective activity as evidenced by the significant and dose dependent restoring the activities of entire liver cancer marker enzymes, diminution in tumor incidence, decrease in lipid peroxidation (LPO) and increase in the level of antioxidant enzymes (GSH, CAT, SOD, GPx and GST) through scavenging of free radicals, or by enhancing the activity of antioxidant, which then detoxify free radicals. These factors protect cells from ROS damage in NDEA and CCl4-induced hepatocarcinogenesis. Histopathological observations of liver tissues too correlated with the biochemical observations. Thus, present investigation suggested that the Fumaria indica would exert a chemoprotective effect by reversing the oxidant-antioxidant imbalance during hepatocarcinogenesis induced by NDEA and CCl4. Besides Fumaria indicais very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various liver injury and biochemical tumour markers enzymes. The hepatoprotective activity of a Fumaria indicaof 50 % ethanolic extract was studied using rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt. Fumaria indica extract dose dependently and significantly the increase in serum hepatic enzyme levels after NDEAand CCl4 treatment compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the Fumaria indicaextract against carbon tetrachlorideand N-nitrosodiethylamine induced hepatotoxicity in rats. In addition to this, studies on molecular aspect of hepatoprotective therapy will give mechanistic information in hepatoprotective therapy and also critical balance should be there between the animal model and clinical research. The hepatoprotective properties of Fumaria indicashould provide useful information in the possible application in hepatic liver disease.

Biological and Phytochemical Screening of Tephrosia purpurea extract on Chemically Induced Hepatocellular Carcinoma with Reference to Biochemical Parameters

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Irfan Aziz ◽  
Birendra Shrivastava ◽  
Chandana Venkateswara Rao ◽  
Sadath Ali
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Marker Enzymes ◽  
Tephrosia Purpurea ◽  
Single Intraperitoneal Injection ◽  
Chemically Induced ◽  
Ccl4 Treatment ◽  
Dose Dependent

Tephrosia purpurea possesses hepatoprotective activity as evidenced by the significant and dose dependent restoring the activities of entire liver cancer marker enzymes, diminution in tumor incidence, decrease in lipid peroxidation (LPO) and increase in the level of antioxidant enzymes (GSH, CAT, SOD, GPx and GST) through scavenging of free radicals, or by enhancing the activity of antioxidant, which then detoxify free radicals. These factors protect cells from ROS damage in NDEA and CCl4-induced hepatocarcinogenesis. Histopathological observations of liver tissues too correlated with the biochemical observations. Thus, present investigation suggested that the Tephrosia purpurea would exert a chemoprotective effect by reversing the oxidant-antioxidant imbalance during hepatocarcinogenesis induced by NDEA and CCl4. Besides Tephrosia purpurea is very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various liver injury and biochemical tumour markers enzymes. The hepatoprotective activity of aTephrosia purpurea of 50 % ethanolic extract was studied using rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt.Tephrosia purpureaextract dose dependently and significantly the increase in serum hepatic enzyme levels after NDEAand CCl4 treatment compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the Tephrosia purpurea extract against carbon tetrachlorideand N-nitrosodiethylamine induced hepatotoxicity in rats.

scholarly journals The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol

2017 ◽  
Vol 15 (2) ◽  
pp. 196
Author(s):  
Much Ilham Novalisa Aji Wibowo ◽  
Nur Aeni ◽  
Zidna Mazayatul Huda ◽  
Nunuk Aries Nurulita
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Group Iv ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Male Wistar Rats ◽  
Hepatoprotective Agent ◽  
Better Than

Syzygium campanulatum and Syzygium aromaticum contains antioxidant components suchas flavonoids, phenolic, and terpenoids. May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotec Syzygium campanulatum and Syzygium aromaticum contains antioxidant components such as flavonoids, phenolic, and terpenoids.May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotective activity of Syzygium aromaticum extracts eff ective as with curcuma tablets at all dosage variation.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA FRUIT AGAINST ETHANOL INDUCED HEPATOTOXICITY IN RATS

2017 ◽  
Vol 10 (11) ◽  
pp. 55
Author(s):  
Balakrishna Vuyyala ◽  
Lakshmi Thakkalapally
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Standard Group ◽  
Terminalia Chebula ◽  
Fruit Extract ◽  
Treatment Protocols ◽  
Standard Drug ◽  
Antioxidant Parameters

  Objective: The purpose of this study was to evaluate the effect of Terminalia chebula fruit extract on liver antioxidant enzymes in ethanol-induced hepatotoxicity in rats.Method: Rats were divided into six different groups each having six. Group 1 served as a control, Group 2 received 40% ethanol (2 ml/100 g, oral), in sterile water, Groups 4, 5, and 6 served as extract treatment groups and received 50, 100, and 200 mg/kg, orally, ethanolic fruit extract of T. chebula (TCE) and Group 3 served as standard group and received silymarin 25 mg/kg orally. All the treatment protocols followed 21 days, and after which rats were sacrificed, the liver was taken for antioxidant and histological studies, respectively.Results: The ethanol-treated group rats (G2) showed variable decrease in antioxidant parameter (catalase, glutathione, and glutathione reductase) levels. Administration of ethanolic TCE significantly prevented ethanol-induced elevation in the levels of malondialdehyde lipid peroxidation and decreased antioxidant parameters in experimental groups of rats. The effect of extract was compared with a standard drug, silymarin. The changes in antioxidant parameters were supported by histological profile.Conclusion: It is concluded that the ethanolic fruit TCE protects against ethanol-induced oxidative liver injury in rats.

scholarly journals Potential New Biomarkers Discovery of Early Nonalcoholic Fatty Liver Disease in Human by Liquid Chromatography–Mass Spectrometry

2020 ◽  
Author(s):  
Cheng Hu ◽  
Tao Wang ◽  
Jiaqi Zhang ◽  
Yuanye Jiang ◽  
Qin Cao
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Fatty Liver Disease ◽  
Early Stage ◽  
Control Group ◽  
Chinese Society ◽  
Nonalcoholic Fatty Liver ◽  
Significant Difference ◽  
Potential Biomarkers

Abstract BackgroundNAFLD is a common metabolic disorders disease which influenced 20~30% people. NAFLD can progress to cirrhosis, liver fibrosis and even liver cancer. Liver puncture is the gold standard. However, due to its trauma and possible complications, its clinical use is currently limited. Therefore, it is of great clinical significance and value to find a noninvasive biochemical index that can diagnose NAFLD early.ObjectiveOur aim was to identify the potential biomarkers in NAFLD people in early stage via untargeted metabolomics study.MethodsIn our research, From January to October 2019, 224 patients aged 18-55 were selected from the outpatient department and ward of gastroenterology department of Putuo Hospital in Shanghai.According to the NAFLD diagnostic criteria of the guidelines for diagnosis and treatment of nonalcoholic fatty liver disease (2018) formulated by the National workship on Fatty liver and alcoholic liver disease and Chinese Society on Hepatology, they were divided into the healthy control group and the experimental group.Besides,on the same day, the height, weight, waist, BMI, blood pressure and heart rate of patients were measured, and fasting blood was taken to obtain blood glucose,ALT, AST, TB, DB, TP, ALB, Che, ALP, γ - GT, TG, TC, HDL-C, LDL-C and other serum data.Serum samples were analyzed using LC/MS and data was processed by SIEVE software and simca-P to validate the potential biomarkers. The altered metabolites were identified by variable importance in projection value (VIP > 1) and ANOVA (p<0.01). The pathway analysis was performed by using MetaboAnalyst 4.0. In addition, our project has passed the review of ethics committee of Putuo Hospital Affiliated to Shanghai University of traditional Chinese medicine, and its ethics approval number is ptec-a-2018-49-1.ResultsThe serum biochemical indicators of early NAFLD patients showed no significant difference with NC (p>0.05). While there was significant difference of blood lipids indicators between NAFLD and NC (p<0.001). Finally, 55 metabolites were identified and the AUC of ROC curve results showed that new identified biomarkers owned high predictability and reliability. ConclusionIt is found that 15 metabolites in serum were of great diagnostic value in early NAFLD patients. AUC of these biomarkers (>0.9) were much higher than clinical indicators (0.770). This may be worth further research in the clinic.

scholarly journals Metabolic analysis of early nonalcoholic fatty liver disease in humans using liquid chromatography-mass spectrometry

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Cheng Hu ◽  
Tao Wang ◽  
Xiaoyu Zhuang ◽  
Qiaoli Sun ◽  
Xiaochun Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Clinical Significance ◽  
Fatty Liver Disease ◽  
Early Stage ◽  
Control Group ◽  
Nonalcoholic Fatty Liver

Abstract Background Nonalcoholic fatty liver disease (NAFLD) is a common metabolic disease that affects 20–30% of individuals worldwide. Liver puncture remains the gold standard for the diagnosis of liver diseases despite limitations regarding invasive nature and sample variability. It is of great clinical significance to find noninvasive biomarkers to detect and predict NAFLD. Objective The aims of this study were to identify potential serum markers in individuals with early-stage NAFLD and to advance the mechanistic understanding of this disease using a high-throughput mass spectrometry-based untargeted metabolomics approach. Methods One hundred and twelve patients with early-stage NAFLD aged 18–55 were recruited according to the guidelines. The control group included 112 healthy participants. The demographic, anthropometric, clinical and laboratory data of all participants were systematically collected. Serum samples were obtained after an overnight fast. The comprehensive serum metabolomic analysis was performed by ultra-performance liquid chromatography-Orbitrap mass spectrometry. The resultant data was processed by Compound Discover and SIMCA-P software to validate the potential biomarkers. Significantly altered metabolites were evaluated by variable importance in projection value (VIP > 1) and ANOVA (p < 0.01). Pathway analysis was performed using MetaboAnalyst 4.0. Results The liver function test of early NAFLD patients showed no statistical differences to control group (p > 0.05). However, obvious differences in blood lipids were observed between subjects with NAFLD and controls (p < 0.001). In total, 55 metabolites showed significant changes in experimental group were identified. The area under curve (AUC) values deduced by receiver operating curve (ROC) analysis indicated that these newly identified biomarkers have high predictability and reliability. Of these, 15 metabolites with AUC greater than 0.9 were of great diagnostic value in early NAFLD patients. Conclusion In this study, a total of 15 serum metabolites were found to strongly associate with early NAFLD. These biomarkers may have great clinical significance in the early diagnosis of NAFLD, as well as to follow response to therapeutic interventions.

Assessment of hepatoprotective activity of coded plant (222) leaf ethanolic extract against carbon tetrachloride-induced hepatotoxicity in Wistar rats – Part IV

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Krishnakumar N M
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Parameters ◽  
Subcutaneous Administration ◽  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Albino Rats ◽  
Hepatic Enzymes ◽  
Standard Drug

The present study was designed to assess the possible hepatoprotective activity of the leaf ethanolic extract of coded plant (Code No. 222**) against carbon tetrachloride (CCl4)-induced hepatic injury in Wistar albino rats. The animals were divided into different groups and treated with 222 leaf ethanolic extract at different concentrations for five days. Silymarin, the known hepatoprotective standard compound (100 mg/kg) was administered for five days. Hepatotoxicity was induced by the subcutaneous administration of a single dose of CCl4: Olive oil (2 mL/kg) on days 2 and 3. The administration of CCl4 resulted in marked increase in serum hepatic enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum bilirubin levels. CCl4 intoxication also resulted in a significant (P=0.05) increase in malondialdehyde (MDA), which is a common marker of lipid peroxidation. The other biochemical parameters such as cholesterol, triglycerides, creatinine, urea and uric acid levels were also increased significantly (P=0.05) compared to normal control group. Changes in serum hepatic enzymes, biochemical parameters and MDA levels induced by CCl4 were reversed by the leaf ethanolic extract of 222 (125 mg/kg) dose. The standard drug silymarin treated group also reversed CCl4-induced changes in biomarkers of liver function and MDA levels. Histopathological studies of the liver samples confirmed the hepatoprotective property of the coded drug 222. It was seen that histopathological damage induced by CCl4 were improved in rat liver, treated with 222 extract. The results of the present study suggested that coded plant (222) leaf ethanolic extract may be used as a hepatoprotective agent against toxic effects caused carbon tetrachloride in the liver.

scholarly journals The Effect of Ethanolic Extract of Roselle (Hibiscuss sabdariffa L) on Increasing SOD Activity

2016 ◽  
Vol 5 (1) ◽  
pp. 118
Author(s):  
Abdul Rahman W ◽  
Nurkhasanah Nurkhasanah ◽  
Nanik Sulistyani
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Activity Data ◽  
Group Iii

Free radicals were reactive species caused oxidation of lipids membrane and DNA. Superoxide dismutase (SOD) is one of the primary antioxidants to inhibit free radicals. The purpose of this study was to determine the effect of ethanol extract of roselle calyx on SOD enzyme activity of the Sprague Dawley (SD) rats induced by 7.12-dimethylbenzen[a]anthracene (DMBA). The animals age of four weeks divided in to five groups, groups I were normal group. Group II is the negative control group only induced by DMBA 75 mg/kgBW; and group III ,IV , and V were treatment groups that treated by ethanolic extract of roselle calyx at dose of 10, 50 and 100 mg/kgBW/day for 7 days. After treatment with roselle, animals were induced by DMBA 75 mg/kgBW. On day 8 after DMBA induction, animals were fasted for 16 hours and blood was collected to measured SOD activity. Data were analyzed using One Way Analysis Of Variance (ANOVA) and LSD (P &lt;0.05). The result showed the significancies on increasing of SOD activity at 10 mg/kgBW (50.87±1.98), 50 mg/kgBW (69.98±3.58) and 100 mg/kgBW (73.01±6.95) compared with DMBA (43.74±1.95) (p&lt;0,05).

scholarly journals HEPATOPROTECTIVE ACTIVITY FROM ETHANOL EXTRACT OF PUGUN TANO’S LEAVE (Curanga fel-terrae (Lour.) Merr.)

2017 ◽  
Vol 10 (11) ◽  
pp. 78
Author(s):  
Urip Harahap ◽  
Marianne Bastian ◽  
Sri Yuliasmi ◽  
Dadang Irfan Husori ◽  
Popi Patilaya ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Negative Control Group ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Normal Group ◽  
Control Group ◽  
High Dose

Objective: The aim of this study is to observe the activity of ethanol extract of Curanga fel-terrae leave in preventing the damaged of liver which is induced by high dose of paracetamol. Methods: This research was conducted by using Wistar rat divided into 6 groups. Group 1 was the normal group. Group 2, 3, 4, 5 and 6 received CMC-Na 0.5% (negative control), C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg, catechin 2 mg/kg (positive control),respectively during 7 days continued and followed by given paracetamol dose of 2.5g/kg 8 hours after that. Hepatoprotective activity was carried out toward parameter of AST, ALT as well as histopathology of the liver. Results: The results showed that high-dose paracetamol dose of 2.5g/kg bw can cause liver damaged which can be seen by the increasing of the level of AST and ALT compared to the normal group (p<0.05). The usage of three doses of CFEEfor 7 days showed the prevention of the increasing of the level of AST and ALT compared to negative control group (p<0.05). Furthermore, the histopathology study revealed that the three doses of extract could protect the liver. Conclusion: The C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg bw which was given for 7 days can prevent the liver from the damage caused by high-dose of paracetamol. Keyword: Curanga fel-terrae (Lour.) Merr., paracetamol, liver, hepatoprotective   

scholarly journals Ficus septica Burm. F. Leaves Ethanolic Extract Induces Apoptosis in 7,12-Dimethylbenz[A]Nthracene-Induced Rat Liver Cancer Quatitavely

2011 ◽  
Vol 2 (2) ◽  
pp. 255 ◽  
Author(s):  
Dita Brenna Septhea ◽  
Anindyajati Anindyajati ◽  
Andita Pra Darma ◽  
Ika Nurzijah ◽  
Agung Endro Nugroho ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Rat Liver ◽  
Control Diet ◽  
Ethanolic Extract ◽  
Diet Group ◽  
Control Group ◽  
Sprague Dawley ◽  
P53 Expression ◽  
Group 5 ◽  
Group 2

The chemopreventive effect of Ficus septica Burm. f. leaves ethanolic extract (FLEE) was studied in 7,12-dimethylbenz[a]nthracene(DMBA)-induced rat liver cancer. Rats were divided into 5 group, 5 rats (5 wk of age Sprague Dawley rat) in each group. Group 1 was control diet group, administered with 0,5% CMC-Na as vehicle. FLEE was administered 750 mg/kgBW and 1500 mg/kgBW starting 4 wk until 5 wk after DMBA administration at the first until fifth wk to group 2 and group 3. Group 4 was control extract group, administered  with 750 mg/kgBW and group 5 was DMBA group. DMBA is a carcinogen to induce liver cancer was also administered in DMBA control group and all animals were necropsied at 6 wk after DMBA administration. Activity of inducing apoptosis was detected using Double Staining method in 750 mg/kgBW FLEE group compared to control group but no in 1500 mg/kgBW FLEE group resulted in 100% dead. Apoptotic cells would have orange flourescence but normal cells would have green flourescence detected by flourescence microscope. To investigate the protein that involved in apoptotic mechanism, we studied p53 expression using Imunohistochemistry (IHC). There was no difference expression of p53 in both tested and control groups. Based on the results, FLEE has a potency as chemoprentive agent because its activity on inducing apoptosis in liver cancer with p53-independent pathway. The mechanism of apoptosis induction of this extract needs to be explored by observing the expression of related proteins.Keywords: apoptosis, Ficus septica, liver cancer, p53 independent pathway

scholarly journals The Effect of Ethanolic Extract of Roselle (Hibiscuss sabdariffa L) on Increasing SOD Activity

2016 ◽  
Vol 5 (1) ◽  
pp. 118
Author(s):  
Abdul Rahman W ◽  
Nurkhasanah Nurkhasanah ◽  
Nanik Sulistyani
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Activity Data ◽  
Group Iii

Free radicals were reactive species caused oxidation of lipids membrane and DNA. Superoxide dismutase (SOD) is one of the primary antioxidants to inhibit free radicals. The purpose of this study was to determine the effect of ethanol extract of roselle calyx on SOD enzyme activity of the Sprague Dawley (SD) rats induced by 7.12-dimethylbenzen[a]anthracene (DMBA). The animals age of four weeks divided in to five groups, groups I were normal group. Group II is the negative control group only induced by DMBA 75 mg/kgBW; and group III ,IV ,and V were treatment groups that treated by ethanolic extract of roselle calyx at dose of 10, 50 and 100 mg/kgBW/day for 7 days. After treatment with roselle, animals were induced by DMBA 75 mg/kgBW. On day 8 after DMBA induction, animals were fasted for 16 hours and blood was collected to measured SOD activity. Data were analyzed using One Way Analysis Of Variance (ANOVA) and LSD (P &lt;0.05). The result showed the significancies on increasing of SOD activity at 10 mg/kgBW (50.87±1.98), 50 mg/kgBW (69.98±3.58) and 100 mg/kgBW (73.01±6.95) compared with DMBA (43.74±1.95) (p&lt;0,05).

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