scholarly journals Neurotrophic factors combined with stem cells in the treatment of sciatic nerve injury in rats:a meta-analysis

2021 ◽  
Author(s):  
Kuangpin Liu ◽  
Wei Ma ◽  
Chun-Yan Li ◽  
Li-Yan Li
Keyword(s):  
Stem Cells ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Neurotrophic Factors ◽  
Neurotrophic Factor ◽  
Meta Analysis ◽  
Peripheral Nerve Regeneration ◽  
Sciatic Nerve Injury

Treatment of peripheral nerve regeneration with stem cells alone has some limitations. For this reason, we evaluate the efficacy of neurotrophic factors combined with stem cell transplantation in the treatment of sciatic nerve injury in rats. PubMed, Cochrane Library, EMbase, WanFang, VIP and China National Knowledge Infrastructure databases were retrieved from inception to October 2021, and control experiments on neurotrophic factors combined with stem cells in the treatment of sciatic nerve injury in rats were searched. Nine articles and 551 rats were included in the meta-analysis. The results of meta-analysis confirmed that neurotrophic factor combined with stem cells for the treatment of sciatic nerve injury yielded more effective repair than normal rats with regard to sciatic nerve index, electrophysiological detection index, electron microscope observation index, and recovery rate of muscle wet weight. The conclusion is that neurotrophic factor combined with stem cells is more conducive to peripheral nerve regeneration and functional recovery than stem cells alone. However, due to the limitation of the quality of the included literature, the above conclusions need to be verified by randomized controlled experiments with higher quality and larger samples.

scholarly journals Transplantation effects of dental pulp-derived cells on peripheral nerve regeneration in crushed sciatic nerve injury

2018 ◽  
Vol 60 (4) ◽  
pp. 526-535 ◽  
Author(s):  
Yuta Okuwa ◽  
Taku Toriumi ◽  
Hidenori Nakayama ◽  
Tatsuaki Ito ◽  
Keita Otake ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Dental Pulp ◽  
Peripheral Nerve Regeneration ◽  
Sciatic Nerve Injury

scholarly journals Schwann Cell-Like Cells Derived from Human Amniotic Mesenchymal Stem Cells Promote Peripheral Nerve Regeneration through a MicroRNA-214/c-Jun Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Wei Chen ◽  
Shune Xiao ◽  
Zairong Wei ◽  
Chengliang Deng ◽  
Kaiyu Nie ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Molecular Mechanisms ◽  
Peripheral Nerve Regeneration ◽  
Sciatic Nerve Injury ◽  
Amniotic Mesenchymal Stem Cells

Background. The use of Schwann cell-like cells (SCLCs) derived from stem cells has been introduced as an effective strategy for promoting peripheral nerve regeneration (PNR). However, molecular mechanisms underlying therapeutic transplantation of SCLCs for PNR are often ignored. Objectives. To explore the potential of SCLCs for the treatment of sciatic never injury and investigate the underlying molecule mechanisms. Method. SCLCs differentiated from human amniotic mesenchymal stem cells (hAMSCs) and specific markers of Schwann cells were detected. SCLCs were transplanted into the injured sites of a rat model of sciatic nerve injury, and sciatic nerve functional index (SFI) was determined. Results. SCLCs expressed specific markers of Schwann cells as well as secreted neurotrophic factors. The transplantation of SCLCs into injured sites of a rat model of sciatic nerve injury promoted the functional recovery. With regard to the underlying molecular mechanisms, we identified c-Jun as a negative regulator of the myelination of SCLCs. Moreover, we discovered a novel signaling transduction pathway in SCLCs; that is, miR-214 directly targets c-Jun to promote the myelination of SCLCs. Finally, we demonstrated that miR-214 upon overexpression in SCLCs enhanced the therapeutic effects of SCLCs on sciatic nerve injury. Conclusions. We demonstrate that SCLCs have beneficial effect for myelination. Moreover, our results provide a previously unknown molecular basis underlying the treatment of peripheral nerve injury with SCLCs and also offer a practical strategy for future therapeutic promotion of PNR.

scholarly journals Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve Regeneration

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Feng Rao ◽  
Dianying Zhang ◽  
Tengjiaozi Fang ◽  
Changfeng Lu ◽  
Bo Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Peripheral Nerve Regeneration

At present, repair methods for peripheral nerve injury often fail to get satisfactory result. Although various strategies have been adopted to investigate the microenvironment after peripheral nerve injury, the underlying molecular mechanisms of neurite outgrowth remain unclear. In this study, we evaluate the effects of exosomes from gingival mesenchymal stem cells (GMSCs) combined with biodegradable chitin conduits on peripheral nerve regeneration. GMSCs were isolated from human gingival tissue and characterized by surface antigen analysis and in vitro multipotent differentiation. The cell supernatant was collected to isolate the exosomes. The exosomes were characterized by transmission electron microscopy, Western blot, and size distribution analysis. The effects of exosomes on peripheral nerve regeneration in vitro were evaluated by coculture with Schwann cells and DRGs. The chitin conduit was prepared and combined with the exosomes to repair rat sciatic nerve defect. Histology, electrophysiology, and gait analysis were used to test the effects of exosomes on sciatic nerve function recovery in vivo. We have successfully cultured GMSCs and isolated exosomes. The exosomes from GMSCs could significantly promote Schwann cell proliferation and DRG axon growth. The in vivo studies showed that chitin conduit combined with exosomes from GMSCs could significantly increase the number and diameter of nerve fibers and promote myelin formation. In addition, muscle function, nerve conduction function, and motor function were also obviously recovered. In summary, this study suggests that GMSC-derived exosomes combined with biodegradable chitin conduits are a useful and novel therapeutic intervention in peripheral nerve repair.

scholarly journals Dexmedetomidine to Help Nerve Regeneration in a Rat Sciatic Nerve Injury Model

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Wook Jeong ◽  
Hsichiang Kung ◽  
Chia Chi Cheng ◽  
Changwoo Lim ◽  
Min Jung Jung ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Peripheral Nerve Regeneration ◽  
Sciatic Nerve Injury ◽  
Sprague Dawley ◽  
Neuroprotective Effects ◽  
Adrenoceptor Agonist ◽  
Group 3 ◽  
Group 2

Background. Several studies have shown that dexmedetomidine (DXM), a selective α2-adrenoceptor agonist, also has neuroprotective effects. However, its effect on impaired peripheral nerve regeneration has not been studied. Materials and Methods. Forty-five Sprague-Dawley rats were randomly assigned to three groups: group 1 (control SHAM), group 2 (sciatic nerve injury + normal saline), and group 3 (sciatic nerve injury + DXM). The rats of group 3 were subdivided into the following three groups: DXM 0.5, 6, and 20 μg·kg−1 (groups 3A, 3B, and 3C, resp.). The sciatic nerve injury was assessed for nerve regeneration at 2 and 6 weeks. Results. There were no differences between groups 2 and 3 in their sciatic functional index (SFI) values or histological findings at 2 weeks postinjury. However, SFI differences were statistically significant at 6 weeks postinjury in group 3. The gross findings with H&E staining showed that the number of axons was higher in group 3 than in group 2. There was no histological difference according to the DXM concentration. Conclusion. The coincidental functional and histological assessment results of this study suggest that DXM for 6 weeks positively affects damaged peripheral nerves.

Neural Stem Cells Enhance Nerve Regeneration after Sciatic Nerve Injury in Rats

2012 ◽  
Vol 46 (2) ◽  
pp. 265-274 ◽  
Author(s):  
Lin Xu ◽  
Shuai Zhou ◽  
Guo-Ying Feng ◽  
Lu-Ping Zhang ◽  
Dong-Mei Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Sciatic Nerve ◽  
Neural Stem Cells ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Sciatic Nerve Injury
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