Identification and Characterization of a Novel Meningococcal Autotransporter Protein: A New Virulence Factor?

Abstract In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has revealed that the S locus is highly polymorphic and contains several genes, i.e., SLG, SRK, the as-yet-unidentified pollen S gene(s), and other linked genes. In the present study, we searched for expressed sequences in a 76-kb SLG/SRK region of the S9 haplotype of Brassica campestris (syn. rapa) and identified 10 genes in addition to the four previously identified (SLG9, SRK9, SAE1, and SLL2) in this haplotype. This gene density (1 gene/5.4 kb) suggests that the S locus is embedded in a gene-rich region of the genome. The average G + C content in this region is 32.6%. An En/Spm-type transposon-like element was found downstream of SLG9. Among the genes we identified that had not previously been found to be linked to the S locus were genes encoding a small cysteine-rich protein, a J-domain protein, and an antisilencing protein (ASF1) homologue. The small cysteine-rich protein was similar to a pollen coat protein, named PCP-A1, which had previously been shown to bind SLG.

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Identification and Characterization of the Escherichia coli RecT Protein, a Protein Encoded by the recE Region That Promotes Renaturation of Homologous Single-Stranded DNA:

Journal of Bacteriology ◽

10.1128/jb.175.4.1211a.1993 ◽

1993 ◽

Vol 175 (4) ◽

pp. 1211.1-1211

Author(s):

Sharynn D. Hall ◽

Michael F. Kane ◽

Richard D. Kolodner

Keyword(s):

Escherichia Coli ◽

Protein A ◽

Single Stranded Dna ◽

Identification And Characterization

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Identification and characterization of virulence factor of several Indonesian Xanthomonas oryzae pv. oryzae

Microbiology Indonesia ◽

10.5454/mi.8.3.3 ◽

2014 ◽

Vol 8 (3) ◽

pp. 103-111

Author(s):

FATIMAH FATIMAH ◽

◽

APON ZAENAL MUSTOPA ◽

IQBAL KUSNANDARSYAH

Keyword(s):

Virulence Factor ◽

Xanthomonas Oryzae ◽

Identification And Characterization

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Bacteriological and Molecular Identification and Characterization of Staphylococcus aureus from Different Affections of Canines

Indian Journal of Animal Research ◽

10.18805/ijar.b-3965 ◽

2020 ◽

Author(s):

Sejal S. Chaudhary ◽

Harshad C. Chauhan ◽

Kishan Kumar Sharma ◽

Sandip S. Patel ◽

Sushil Kumar Mohapatra ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Protein A ◽

Molecular Techniques ◽

Haemolytic Activity ◽

Eye Infections ◽

Respiratory Problems ◽

Spa Gene ◽

Staphylococcus Spp ◽

Identification And Characterization

Background: The present study was done to ascertain prevalence of Staphylococcus aureus from various canine affections in Banaskantha district of Gujarat, India. Along with this, use of classical and molecular techniques were compared in identification and virulence characterization of this pathogen.Methods: A total of 165 samples were collected and bacterial identification was carried out with bacteriological (phenotypic) techniques and confirmed by genus specific 16S rDNA and Staphylococcus aureus specific sa442 gene based PCR. Isolates were characterized for coagulase production, haemolysis activity and presence of spa gene. Result: Samples yielded, 88 (53.33%) Staphylococcus spp. via bacteriological and PCR methods. Clinically, 19 (21.59%), 28 (31.82%), 12 (13.64%), 15 (17.04%) and 14 (15.91%) isolates were from abscess/wound, pyoderma, respiratory problems, eye infections and Otitis, respectively. A total of 46/88 (52.27%) isolates were confirmed as Staphylococcus aureus in PCR. In tube coagulase test, 51/88 (57.95%) isolates were found positive. A tota of 42 isolates revealed presence of coa gene, including two tube coagulase negative isolates. Haemolytic activity revealed beta (51.14%) gamma (31.82%), alpha (13.64%) and alpha-beta (3.41%) haemolysis, respectively. X-region of Protein A (spa gene) was detected in 26 /46 (56.52%) isolates in PCR.

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